Labshake search
Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... total cellular RNA was extracted from Huh-7 cells treated with oleic acid for 24 hours using TRIZOL reagent (ThermoFisher Scientific) and reverse transcribed using the Superscript VILO (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then resuspended in DMEM + 10% FBS supplemented with 50 μM CellEvent™ Caspase 3/7 Green Detection Reagent (Invitrogen) at 37 °C for 25 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Real-time qPCR was performed using SYBR Green (BIO-RAD and a ViiA 7 Real Time System (ThermoFisher, Waltham, MA, USA) as specified by the manufacturer ...
-
bioRxiv - Microbiology 2021Quote: Huh-7 cells were transfected with the HBsAg or luciferase reporter derived plasmids per manufacturer’s instructions (Lipofectamine 3000, Invitrogen, MA, USA). Cells were treated with the indicated compounds for 5 days ...
-
bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed using an UltraSYBR Mixture (Beijing CoWin Biotech, Beijing, China) on a ViiA 7 real-time PCR system (Applied Biosystems). Viral rRNAs were normalized to swine 28S rRNA expression ...
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Neuroscience 2020Quote: ... PR1/2 and mPRs genes were determined through quantitative PCR (qPCR) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems), and data were analyzed with DataAssist (ABI ...
-
bioRxiv - Microbiology 2020Quote: Pseudo-typed viruses were produced as previously described (50), 293T (ATCC, CRL-3216), Huh-7 and Vero (ATCC, CCL-81) cells were maintained in high glucose DMEM (Gibco, USA) supplemented with 10% FBS (FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurospheres were passaged by centrifuging 5 min at 1,000 rpm and incubating for 7 min in Accutase (Gibco® A11105-01) before mechanically dissociating the spheres into single-cell suspension.
-
bioRxiv - Microbiology 2020Quote: ... di- and oligo- saccharides present in the spent media samples were analyzed on a Dionex ICS-5000 HPAEC system operated by the Chromeleon software version 7 (Dionex, Thermo Scientific). Samples were bound to a Dionex CarboPac PA1 (Thermo Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Murine PNPLA2 mRNA levels relative to HPRT transcript levels were measured by the QuantStudio 7 Flex Real-Time PCR System using Taqman® gene expression assays (PNPLA2, Mm00503040_m1; HPRT, Mm00446968_m1, Thermo Fisher Scientific). PNPLA2 relative expression to HPRT was calculated using the comparative ΔΔCt method.27
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Neuroscience 2022Quote: For insulin and rapamycin treatment cells were seeded at 7 × 105 cells per well in a six-well plate (Thermo Scientific) in DMEM + 10% FBS ...
-
bioRxiv - Immunology 2022Quote: ... cDNA was amplified using the Fast SYBR Green Master Mix on a QuantStudio 7 Flex Real Time PCR Systems (Applied Biosystems). The sequences of primer pairs (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: About 80,000 MCF-7 cells were placed in each chamber of a 4-well chamber slide (Thermo Scientific, cat. no. 177399) containing 500 μl of Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Immunology 2022Quote: ... and Il-1β (in aortic CD3- cells) mRNA levels were assessed by qPCR using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems), and initial differences in RNA quantity were corrected for using the housekeeping genes 36b4 and Cyclophilin B.
-
bioRxiv - Cancer Biology 2022Quote: ... Cell number per well by DNA content was determined after 7-10 days of culture using CyQUANT Direct Cell Proliferation Assay (Life Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2022Quote: ... PCR was performed for 55-60 cycles for maximal saturation of signal with final extension at 72 °C for 7 min in a 7500 Fast Real-Time PCR System (Applied Biosystems). The primer sequences used were ...
-
bioRxiv - Developmental Biology 2019Quote: ... Dead cells were excluded based on forward and side scatter profiles and the uptake of 7AAD (7-Aminoactinomycin D dead cell stain, Life Technologies). Sorted YFP-positive and YFP-negative cells were pelleted by centrifugation and flash frozen in liquid nitrogen ...
-
bioRxiv - Neuroscience 2019Quote: ... The PCR amplification was performed using Power SYBR green dye and ViiA 7 Real-Time PCR system (Applied Biosystems, Carlsbad, CA). All primer sets used in the qPCR analysis (Table 3 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cos-7, 293T cells were obtained from The American Type Culture Collection (Manassas, Virginia) and 293FT cells were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... Real-time PCR was performed on the QuantStudio 7 Flex Real-Time PCR System using Power SYBR Green PCR Master Mix (Applied Biosystems). The PCR conditions were 950C for 10 min ...
-
Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... A pre-warmed buffer containing DPBS supplemented with 10% FBS and CellEvent Caspase 3/7 Green (1 μL/1 mL of buffer; ThermoFisher C10427) were added to the cells ...
-
bioRxiv - Molecular Biology 2019Quote: ... for each reaction was incorporated into the standard curve to derive the number of mRNA molecules present using the ViiA 7 software (Applied Biosystems). The standard curve was generated using 13 ...
-
bioRxiv - Biophysics 2019Quote: ... The grids were blotted for 4 s with blot force 7 at 85% humidity and 15°C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). Datasets were collected using a Titan Krios operated at an acceleration voltage of 300 keV and the GATAN K2 Summit direct electron detector coupled with the GIF quantum energy filter (Gatan Inc. ...
-
bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
-
bioRxiv - Microbiology 2019Quote: ... post-stained in saturated Uranyl acetate in 70% Ethanol (Roth) for 7 minutes and subsequently imaged using an FEI Tecnai F20 electron microscope (Thermofisher, USA).
-
bioRxiv - Biochemistry 2020Quote: ... The DNA was diluted to 40 μM and was incubated with 7 U/mL DNase I (Thermo Fisher Scientific, Waltham, MA), 10 mM MES pH 5.5 or 10 mM Tris pH 7.5 ...
-
bioRxiv - Genomics 2020Quote: ... 4 μM glass slide) then for Laser Capture Microdissection (LCM; N=3; 7 μM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522). The slides were stored at −20°C in an airtight container with desiccant until ready for dissection (1 day to 3 months) ...
-
bioRxiv - Immunology 2019Quote: ... and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Frozen PBMCs were stored at −80°C before shipping on dry ice to Benaroya Research Institute for further processing and stimulation as described above.
-
bioRxiv - Immunology 2019Quote: ... one half being used immediately and the other half frozen in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Freshly isolated PBMCs were plated in 96 deep-well plates (Greiner Bio-One ...
-
bioRxiv - Genomics 2020Quote: ... All four RT-PCR reactions were performed for each sample in duplicate using the following cycling conditions on an Applied Biosystems QuantStudio 7 Instrument (Applied Biosystems): reverse transcription at 50 °C for 5 min ...
-
bioRxiv - Bioengineering 2020Quote: ... was carried out for 7 and 21 days in a 12-well plate in the nutrient medium containing DMEM medium (Gibco, USA), 10% of fetal bovine serum (Gibco ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2019-nCoV RUO Kit (Integrated DNA Technologies) and 2019-nCoV_N positive Control (Integrated DNA Technologies) with a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2020Quote: The in vitro enzymatic assay with the fluorogenic substrate ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, UbiQ) was assembled in a 384-well microplate (Thermo Scientific). Assays were performed in 20 μL reaction volumes and in triplicate ...
-
bioRxiv - Neuroscience 2020Quote: ... ATCC) and human kidney COS-7 cells (CRL-1651, ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco 11995-065) supplemented with 10% v/v heat-inactivated fetal bovine serum (Gibco 12483-020 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... University of Nottingham) and Cos-7 (ATCC) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich or Thermo Fisher Scientific) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples with an integrity number higher than 7 were used for cDNA synthesis using the Superscript III RT kit (Invitrogen, Germany). Real-time semiquantitative PCR with TaqMan and SYBRGreen were performed in the LightCycler 480 Systems (384-well format ...
-
bioRxiv - Neuroscience 2020Quote: ... were collected from FB labelled mice 7-10 days after knee injections in ice cold dissociation media containing L-15 Medium (1X) + GlutaMAX-l (Life Technologies), supplemented with 24 mM NaHCO3 ...
-
bioRxiv - Microbiology 2019Quote: ... HeLa and MCF-7 cells (ATCC) were cultured in the same medium supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher) instead of Nu serum ...
-
bioRxiv - Genomics 2021Quote: ... ChIP DNA samples were amplified on an Applied Biosystems real-time PCR machine using site-specific primer sets (Supplementary Table 7) and quantified using SYBR Green (Applied Biosystems) incorporation ...
-
bioRxiv - Immunology 2021Quote: ... a T-cell proliferation assay was performed by incubating collected mice spleen cells for 7 min at RT with 5 μM Carboxyfluorescein succinimidyl ester (CFSE) (Life Technologies), staining was quenched with FCS and splenocytes cultured at 106 cells/well in 96-well plates for 5 days at 37°C in 5% CO2 with 3 μg/ml of rSp antigen ...
-
bioRxiv - Evolutionary Biology 2021Quote: For ethanol preference assays, experimental substrates were 1% agarose and contained 75mM sucrose and increasing concentrations (3%, 5%, and 7%) of ethanol (ThermoFisher #BP2818); control substrates were 1% agarose and contained 75mM sucrose.
-
bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
-
bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
-
bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
-
bioRxiv - Biochemistry 2020Quote: Differential scanning fluorimetry protein thermal shift assays were carried out using a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Reactions were pipetted into wells in a MicroAmp FAST optical 96-well plate (Life Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... at a concentration of 7 μM in the electroporation slurry (as previously described in Alex et al., 2019) (Neon Transfection System, Thermo Fisher). Control cells were electroporated with mCherry or electroporation buffer ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). The following thermal cycling conditions were used ...
-
bioRxiv - Bioengineering 2021Quote: ... The reaction solution was then purified via Zeba spin desalting columns with 7 kDa cutoff to remove unreacted linker (Thermo Fisher). Successful linker conjugation was confirmed using gel electrophoresis and comparison to a size standard of the unmodified Spike ...