Labshake search
Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system (Applied Biosystems). Expression of genes was normalised to three housekeeping genes (Gapdh ...
-
bioRxiv - Molecular Biology 2021Quote: ... and real-time quantitative PCR was performed using a Viia 7 Real-Time PCR System on 384 custom-made TaqMan microfluidic cards (ThermoFisher, 4342253). CTs were normalised to the average CT values of Gapdh and Hprt ...
-
bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cultures on feeder layers were performed on Mitomycin C-treated MEF as described above with passaging every 7 days using 1 mg/mL collagenase IV in DMEM/F12 (Life technologies) the feeder-free culture was performed on Geltrex™ (Life technologies ...
-
bioRxiv - Immunology 2019Quote: ... Then to stain the cells we used antibodies against LFA-1 (clone I21/7) and 4G10 with Alexa Fluor 568–conjugated phalloidin (Life Technologies) and DAPI ...
-
bioRxiv - Developmental Biology 2019Quote: ... The PCR reactions were performed using a Quant Studio 7 Flex Fast Real-Time PCR System (Applied Biosystems by life technologies) and the following Taqman primers (Thermo Fisher) ...
-
bioRxiv - Genomics 2019Quote: ... Gene expression was determined via Taqman qPCR assays (Table S5) and run on the Applied Biosystems ViiATM 7 Real Time PCR system (ThermoFisher Scientific).
-
bioRxiv - Systems Biology 2020Quote: ... and the pellet was resuspended in 7 mL PBS supplemented with 0.01 U/μL SUPERase In RNase Inhibitor (AM2696, Invitrogen, Carlsbad, CA), hereafter referred to as PBS-RI ...
-
bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR analysis using the comparative Ct method was performed on the Applied Biosystem Vii 7 system (ThermoFisher Scientific) with the Powerup SYBR Master Mix (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2019Quote: ... The valve then switches to engage the passive split such that a majority of LC effluent is diverted to waste prior to the third dimension PC. 13 RP-SAX-RP fractions were analyzed. Either an LTQ Velos (Figs. 1, 7) or Q-Exactive HF (Thermo Scientific) machine was used to acquire MS/MS spectra in data-dependent mode as described (Zhang et al. ...
-
bioRxiv - Biophysics 2019Quote: The samples of 40-nm diameter dark red fluorescent beads deposited on a coverslip (Supplementary Fig. 6a, Supplementary Fig. 7) were obtained by diluting the initial solution (10720, Thermo Fisher) at 5.10−7 in PBS + 5% glucose ...
-
bioRxiv - Physiology 2019Quote: Total RNA samples were extracted from one thousand 4-7 day-old Culex female antennae with TRIzol reagent (Invitrogen, Carlsbad, CA). Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 0,25 μM of forward and reverse primers (Table S5) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
-
bioRxiv - Genetics 2019Quote: ... The concentration of amplified cDNA was measured using the Qubit dsDNA High Sensitivity Assay Kit (Life 7 Technologies/Thermo Fisher Scientific). The cDNA size distribution of few selected wells was assessed on a High-Sensitivity Bioanalyzer Chip (Agilent) ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quantitative PCR (QPCR) assays were performed in duplicate using the Applied Biosystems ViiA 7 System (Life Technologies, Grand Island, NY, USA). Housekeeping genes used to normalize the gene expression data were eliminated if associated with independent variables and covariates in analyses of variance (ANOVA) ...
-
bioRxiv - Physiology 2019Quote: ... Quantitative RT-PCR was carried out with either TaqMan™ Universal PCR Master Mix or SYBR Green PCR master mix on the QuantStudio 7 Flex Real time PCR system (Applied Biosystems). All reactions were carried out in either duplicate or triplicate and Ct values were obtained ...
-
bioRxiv - Bioengineering 2019Quote: Matrix remodeling associated gene expression profiles were examined for MSC-seeded hydrogel specimens via QuantiStudio 7 Flex real-time PCR (Applied Biosystems). Frozen hydrogels isolated at days 0 ...
-
bioRxiv - Biochemistry 2021Quote: ... The grids were blotted for 6∼7 s at 100 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher). The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher ...
-
bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
-
bioRxiv - Bioengineering 2021Quote: ... coli Famp (ATCC #700891) suspension were added to 7 mL of top agar [0.5% (w/v) NaCl (Fisher Scientific #S271-500) and 0.7% (w/v ...
-
bioRxiv - Biochemistry 2021Quote: ... cDNA samples were used as templates to perform real- time quantitative PCR with the power SYBR green mix (Abm, Cat#MasterMix-LR) on an Applied Biosystems QuantStudio (TM) 7 Flex System (Applied Biosystems). Duplicate runs of each sample were normalized to 36b4 to determine the relative transcript abundance of target genes ...
-
bioRxiv - Bioengineering 2020Quote: ... from concentrated HTM cell culture supernatants ± DEX at 7 d supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) in 6X loading buffer (Boston Bio Products ...
-
bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies] ...
-
bioRxiv - Cancer Biology 2021Quote: ... H460 or MCF-7 cells with or without increasing numbers of MSCs were seeded in round-bottom 96-well plates (Nunclon Sphera, ThermoFisher Scientific). Living cell numbers and metabolic activity were measured with an MTT assay or labeled for 1 h using the LIVE/DEAD® kit (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: Stable transfections of OCI-LY-7 and Z-138 cells were performed by electroporation using the Neon® Transfection System (Invitrogen). Electroporation settings were selected after 24-well optimization was performed according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: Real-time PCR reactions were performed in triplicate in 96-well plates in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). Equal initial amounts of total DNA were amplified in all conditions ...
-
bioRxiv - Cell Biology 2021Quote: ... A 10 μl aliquot of Spike S1 at 1 mg/ml as provided by the manufacturer was incubated for 20 min at r.t in the presence of a 10-fold molar excess of FITC or a 7-molar excess of Alexa Fluor 488 carboxylic acid succinimidyl ester (CASE) dye (ThermoFisher Scientific). Afterwards ...
-
bioRxiv - Plant Biology 2021Quote: ... insertions were additionally verified by flanking fragment sequencing (Supplementary Fig. 7) using the Phire Plant Direct PCR Kit (Thermo Fisher Scientific) and primers listed in Supplementary Table 1 ...
-
bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
-
bioRxiv - Systems Biology 2021Quote: ... mRNA expression of genes of interest was analyzed with TaqMan real-time PCR in a ViiA™ 7 system (Applied Biosystems). RNA sequencing library were prepared with Illumina RNA-Seq with Poly-A selections ...
-
bioRxiv - Plant Biology 2021Quote: ... and quantification PCRs were performed with ChamQ™ SYBR qPCR Master Mix (both from Vazyme Biotech co. ltd, Nanjing) on QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher). Three to four biological replicates from each of two to three independent trials were applied for each experiment ...
-
bioRxiv - Neuroscience 2020Quote: Adrenal glands and brain extracts were collected from P0-1 Syt-7 WT and KO mice and lysed in RIPA buffer supplemented with Protease Inhibitor Cocktail (Invitrogen, 89900). The supernatants were collected and protein concentrations were estimated by using the BCA Protein Assay Kit (Pierce 23227 ...
-
bioRxiv - Microbiology 2021Quote: ... from Mouse Antibody Isotyping 7-Plex ProcartaPlex were used as analytes and the samples were prepared according to manufacturer’s instructions (Thermo Fisher, UK).
-
bioRxiv - Microbiology 2020Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). Primers were based on previous studies (Ding et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2021Quote: ... qPCR was performed with the ViiA 7™ Real-Time PCR or ABI PRISM 7700 systems (Applied Biosystems, Foster City, CA). Methods are detailed in the online Supplementary material.
-
bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... and homogenized with 1xPBS containing protease and phosphatase inhibitors (Pierce) (7).Protein concentration was analyzed by standard BCA method with a NanoDrop spectrophotometer (ND-1000, Thermo Scientific). A 15% SDS-PAGE was used to run the samples at equal concentrations and transferred to a Immobilon-FL membrane ...
-
bioRxiv - Neuroscience 2022Quote: ... the percentage of apoptotic astrocytes was evaluated using CellEvent Caspase-3/7 Green Detection Reagent (1:250; Thermo Fisher, cat. #C10423) added directly to the medium ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
-
bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio-7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
-
bioRxiv - Bioengineering 2022Quote: ... medium was changed to RPMI + B27-I and on day 7 to RPMI 1640 supplemented with B27 complete supplement (B27C; 17504044, Gibco, USA), which was thereafter used for CM culture ...
-
bioRxiv - Cancer Biology 2022Quote: siRNA oligos targeting human lnc152 [described previously (22)] were transfected into MCF-7 cells at a final concentration of 10 nM using Lipofectamine RNAiMAX reagent (Invitrogen, 13778150) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on the Applied Biosystems ViiA 7 using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The level of ACTIN7 (ACT7 ...
-
bioRxiv - Bioengineering 2022Quote: ... 7 µm sections were cut on a Leica RM 2135 rotary microtome and mounted on Superfrost Plus microscope slides (Fisher Scientific).
-
bioRxiv - Biochemistry 2022Quote: ... at a concentration of 7 μM in the electroporation slurry (as previously described in (Alex et al., 2019)) (Neon Transfection System, Thermo Fisher). Control cells were electroporated with mCherry ...
-
bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (SH30243.01, Hyclone GE) supplemented with 10% FBS (10270-106, Gibco-Thermo Fisher Scientific) and 1% penicillin-streptomycin (A001A ...
-
bioRxiv - Molecular Biology 2020Quote: ... The sonicated material was then pooled together and distributed into 500 μL aliquots (equivalent of 7*108 cells per aliquot) and 25 μl of Protein G magnetic beads (Life Technologies-Invitrogen) pre-bound with 6μg ...
-
bioRxiv - Immunology 2021Quote: ... for 35 minutes at 165V and transferred onto nitrocellulose membranes using the iBlot® 7-Minute Blotting System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...