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Citations for Thermo Fisher :
4051 - 4100 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... using primary antibodies diluted in blocking solution (VE-Cadherin, #14-1441-82, 5 μg/ml final and HES1, #PA5-28802, 5 μg/ml final, Thermo Fisher Scientific) for 48h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated for 2 hours at room temperature (22 ℃) in blocking solution containing the secondary antibodies Alexa Fluor® 555 donkey anti-rabbit (1:500, Thermo Fisher Scientific Cat# A-31570 ...
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bioRxiv - Neuroscience 2023Quote: ... After 1hr of incubation with blocking solution [0.1M tris buffered saline (TBS) with 1% bovine serum albumin (BSA, ThermoFisher Scientific, Waltham MA) and 5% dry milk] ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were processed for 48 hours at 4 degrees with the primary antibody in blocking solution (1:1250, rabbit anti-Esr1, Invitrogen PA1-309), rinsed 3×10 minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Coverslips were washed with blocking solution 3 times at room temperature and incubated in blocking buffer containing Alexa Flour 488 anti-mouse IgG antibody (1:300; Invitrogen, MA, USA) for 1 h at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... The lower molecular weight part of the membrane was incubated overnight at 4ºC in anti-6X-HisTag antibody (1:1000 dilution in blocking buffer, Invitrogen MA1-21315-HRP) to stain for SsrB protein constructs ...
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bioRxiv - Neuroscience 2022Quote: ... Blots to be probed with the #1 antibody were blocked in 5% milk while all others were blocked in Superblock Blocking Buffer (ThermoFisher, Cat#37515), each with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with Odyssey Blocking Buffer (LiCor, USA) before being probed overnight with primary antibodies against C9ORF72 (1:1000, 22637-1-AP, Thermo Scientific, USA). Infrared labeled secondary antibodies were applied ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation in an Alexa Fluor (AF) 488-conjugated goat-anti-rabbit secondary antibodies in the blocking solution (dilution 1:200, ThermoFisher, A-11008) for 2 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ovaries were then stained for 12 hours using the following reagents at 1:500 dilution in blocking solution: Hoechst 33342 (10 mg/ml, Thermo Fisher H3570), Wheat Germ Agglutinin-647 (WGA ...
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bioRxiv - Neuroscience 2024Quote: ... and then cells were washed with ice-cold blocking solution and incubated with secondary antibody (goat anti-rabbit with Alexa Fluor 555, 1:1000, Thermo Fisher Scientific). Cells were then washed with ice-cold PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... All antibodies were diluted in blocking buffer as described in the Bioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00).
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bioRxiv - Microbiology 2024Quote: ... coli type I fimbria or FliC (diluted 1:10 000 in protein-free tris-buffered saline (TBS) blocking solution (Fisher Scientific, France)) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA Fraction V 7.5% (Thermo Fisher Scientific, 15260-037) + 5% goat serum (Jackson Immunoresearch/Dianova ...
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bioRxiv - Molecular Biology 2024Quote: ... sections were washed for 5 minutes in PBS and immediately incubated in blocking solution for 30 minutes (Pierce Super Blocker, Thermo Fisher Scientific). Sections were then incubated in a primary antibody cocktail for 60 minutes containing 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... then the following secondary antibodies were diluted 1:500 in blocking buffer and incubated for 1h with the cells: anti-rabbit DyLight 448 (Thermo Scientific, 35552), anti-rabbit DyLight 550 (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA pol I and II visualization was accomplished by blocking (2) and incubating samples with rabbit anti-p-UBF1 (1:100 dilution; Invitrogen, PA5-105512) and mouse anti-RNA pol II (1:100 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer) for 1 hour at room temperature and detected using SuperSignal Western Dura PLUS Chemiluminescent Substrate (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody goat anti-Rabbit DyLight 680 (ThermoFisher, cat. no. 35568) or/and donkey anti-Goat DyLight 680 (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... then immersed in diluted blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse IgG1, Invitrogen / Thermo-Fisher), each at 1:800 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were diluted in blocking buffer as follows: Alexa Fluor 488-Goat anti-Mouse IgG (H+L) secondary antibody (1:1000; Life Technologies; A11029) an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were incubated for 1 hour in blocking buffer containing goat αrabbit IgG (H+L) secondary antibody Alexa Fluor 568 conjugate (Thermo Fisher Scientific). Again ...
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bioRxiv - Bioengineering 2023Quote: Three vibratome slices from three goat eyes were incubated for 4 hours in 5% BSA blocking solution without permeabilization agent, overnight in GFAP primary antibody (rabbit origin, (Invitrogen, PA5-16291) at 1:200 in blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed for 2 h at RT: Anti-mouse-Alexa 647 (1:500, cat. A32787, ThermoFisher Scientific Inc.), Alexa Fluor 488-Phalloidin (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... followed and the slides were incubated with secondary antibody (1:400 in blocking buffer, donkey a-rabbit Alexa 488 antibody, Invitrogen A-21206; or goat a-mouse Alexa488, Invitrogen A-11001) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed with TBST and incubated with secondary antibodies in blocking buffer [goat anti-rabbit (cat# A32731; Invitrogen, Waltham, MA, USA) or goat anti-mouse (cat# 8890 ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed utilizing the following horseradish peroxidase-conjugated primary antibodies that were diluted 1:1000 in blocking buffer: goat anti-mouse IgG1 (A10551, Thermo Fisher Scientific), goat anti-mouse IgG2c (PA1-29288 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and incubated for 45 min with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Donkey anti-Rabbit IgG, (#A10042, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2022Quote: ... in blocking buffer and then for 2 hours at room temperature with Alexa Fluor–conjugated secondary antibodies (1: 1000, Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were subsequently washed with PBS and incubated with fluorophore-conjugated secondary antibodies in blocking solution [anti-rabbit IgG Alexa Fluor™ 594 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and the following secondary antibodies diluted in blocking buffer: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with blocking buffer and incubated with Alexa Fluor 594 or 568-conjugated streptavidin (ThermoFisher #S32356 or #S11226, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... DRG samples were incubated in the blocking solution with added primary antibodies: anti-GFP (Invitrogen, #11122, 1:400, 2 days, 37°C), and anti-V5 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of Brilliant Stain Buffer Plus (10 μl/sample) (cat# 563795, Becton Dickinson, Mountain View, Ca, USA) and CellBlox Blocking Buffer (5 μl/sample) (cat# C001T02F01, Thermo Fisher Scientific). Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401 ...
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bioRxiv - Cell Biology 2024Quote: ... They were then exposed at room temperature first for 1 hour to primary Abs in blocking buffer and then for 30 minutes to Alexa Fluor–conjugated secondary Abs (Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... the following secondary antibody was added overnight at 4°C in blocking buffer: Alexa FluorTM 647 donkey anti-rabbit IgG (1:1000, Invitrogen #A-32795). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 hour at room temperature and subsequently incubated in the blocking buffer containing rabbit anti-SYNGAP1 (1:5000; ThermoFisher #PA1-046), rabbit anti-SRGAP2A (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific], Donkey anti-mouse Alexa Fluor 488 [A21202; Fisher Scientific] ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 h at room temperature preceding primary antibody incubation using a 1:1 mixture of Odyssey Blocking Buffer and TBST (ThermoFisher, J77500.K8) diluted to 1x concentration at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: The slides were pre-incubated in blocking solution and stained against Griffonia simplicifolia isolectin GS-IB4 Alexa 568 (Invitrogen, I21412; 1:500), Neurofilament 200 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were washed in PBS for 5 minutes and subsequently incubated in blocking solution for 15 minutes (Pierce Super Blocker; Thermo Fisher Scientific). The slides were incubated in primary antibody solution (1x PBS ...