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Citations for Thermo Fisher :
4001 - 4050 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched in 0.5% trifluoroacetic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/μL ...
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bioRxiv - Molecular Biology 2024Quote: The resulting peptide mixtures were analyzed in data-dependent acquisition mode with an Orbitrap Exploris high-resolution mass spectrometer (MS) (ThermoFisher) coupled to an UltiMate 3000 LC system (Dionex-LC Packings) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of peptides for each sample were labeled with 50 µg of TMTpro16-plex reagents (Thermo Fisher Scientific) per channel ...
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bioRxiv - Cancer Biology 2024Quote: The peptide samples (1-1.5 μL) were analyzed with a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray source operating at 2 kV at a capillary temperature of 320 °C and coupled to an EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 4 µg of peptides were analyzed in a QExactive mass spectrometer coupled to a nano EasyLC 1000 (Thermo Fisher Scientific). Solvent composition at the two channels was 0.1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated for 1 hour at room temperature in blocking buffer containing the following secondary antibodies: Goat anti-Rabbit Alexa Fluor® 594 conjugate (1:250, #A-11037, Life Technologies) and Donkey anti-Mouse Alexa Fluor® 488 conjugate (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were permeabilized with 0.5% Triton X-100 in DPBS for 10 minutes and incubated in BlockAid Blocking Solution (Thermo Fisher, Cat# B10710) for 1 h at RT in a humidity chamber and incubated O/N in primary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... NRK cells expressing WT importin α or mutant importin α S62A released from thymidine blocking were changed to CO2-independent medium (Invitrogen), 10% cosmic calf serum (HyClone) ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections of the cochlea and brain slices were washed in PBS for 1 h and subsequently incubated in secondary antibody solution containing blocking solution and the secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit (1:1000, cochlear cryosections) (Molecular Probes (A11008)) ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were then washed three times with 1xPBS and incubated with Alexa 594 conjugated anti-mouse IgG antibody (1:500 in blocking buffer, ThermoFisher Scientific, A11012) and Alexa 488 conjugated anti-rabbit IgG antibody (1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for one hour on the rocker at room temperature in blocking solution with 1:1000 Goat anti-Rabbit IgG H&L Alexa Fluor 405 (Thermo Fisher Scientific). Then sections were washed once in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3×5mins in PBS and incubated for two hours in appropriate secondary antibodies (Invitrogen; 1:250 in blocking solution). For double labelling of Iba1 and GAD67 ...
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bioRxiv - Bioengineering 2019Quote: ... cardiomyocytes were incubated in blocking solution containing Alexa Fluor Plus 488-conjugated goat anti-mouse IgG (1:300, A32723; Thermo Fisher Scientific) for 60 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were rinsed three times with PBS and blocking buffer with AlexaFluor 488-coupled goat anti-mouse antibody (Life Technologies, 1:1000) and 0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... After rinsing in PBS and incubating in blocking buffer (10% normal goat serum, Cat #PCN5000 and 0.2% Triton™ X-100 Cat #9002-93-1, ThermoFisher, in PBS) for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 x 10 min in PBS and incubated in goat anti-rabbit Alexa Fluor 647 (1:500 in blocking buffer; catalog no. A32733, Invitrogen, Carlsbad, CA) for 2 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Molecular Biology 2021Quote: ... coverslips were washed 3 times in PBS + 0.1% Tween then incubated at room temperature in the dark for 1 hour in blocking buffer containing Alexa Fluor 546 goat anti-rabbit IgG (Invitrogen, A-11035) secondary antibody (1:1500 ...
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bioRxiv - Genomics 2021Quote: ... and then blocked for 1 hour with 1 ml of blocking buffer (wash buffer containing 3% fatty acid free BSA (Thermo Fisher, 126609)) ...
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bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% PFA in phosphate-buffered saline (PBS) for 30 min and blocked with blocking buffer consisting of 10% horse serum (Life Technologies, 26050088), 0.1% gelatin ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed twice with PBS and incubated in the blocking buffer containing donkey anti-goat IgG antibodies conjugated with Alexa Fluor 568 (ThermoFisher Scientific, USA) diluted 1:400 for 2 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3×10 mins in IF blocking buffer prior to mounting on to coverslides with ProLong Diamond Antifade Mountant with DAPI (ThermoFisher Scientific #P36962). Slides were allowed to cure overnight before imaging with either a Zeiss AxioObserver widefield microscope (20x ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 x 5m in PBS and then incubated 1h at RT with the following secondary antibodies all diluted 1:1000 in blocking solution: donkey anti-rabbit Alexa Fluor 647 (ThermoFisher A-31573); goat anti-mouse Alexa Fluor 568 (ThermoFisher A-11031) ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary labelling was done for 1 hour in blocking buffer containing Alexa Fluor 488 goat anti-rabbit IgG (InVitrogen, Waltham, MA (A11008)) or Alexa Fluor 488 goat anti-rat (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μg/mL Hoechst 33342 (Fischer Scientific BDB561908) in the donkey blocking solution before washing and mounting on slides with Immu-Mount (Thermo Fisher Scientific) and 1.5 mm coverslips (EMS).
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bioRxiv - Immunology 2022Quote: ... The next day the plates were washed three times with TBS and blocked for 30 min with Casein blocking buffer (Thermo Fisher Scientific). After washing the plates with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were kept in the dark in blocking solution containing donkey anti-rabbit Alexa Fluor ® 647 (1:500 ab150075 Invitrogen®) and Goat anti-mouse Alexa Fluor ® 488 (1:500 A11001 ThermoFisher® ...
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bioRxiv - Bioengineering 2020Quote: ... membranes were incubated in secondary antibody for 1 hr at RT (goat anti-rabbit HRP conjugated, 1:2500 in blocking solution; ThermoFisher Scientific, UK).
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with the blocking buffer and re-suspended in PBS containing 5 mg/ml BSA (Thermo Fisher Scientific, USA), 0.5 U/ml RNsing plus (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated for 1 h at room temperature in blocking solution containing anti-rabbit secondary antibody conjugated to Alexa Fluor 488 (1:250 dilution) (Thermo Fisher Scientific). After an additional 3x washes ...
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bioRxiv - Bioengineering 2022Quote: ... wells were washed five times with PBS and blocked for 30 min with SuperBlock Blocking buffer (Life Technologies Europe BV, Bleiswijk, Netherlands) at 150 μL per well and at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight at 4°C in primary antibodies diluted in blocking buffer and after washing in PBST the blots were developed with the SuperSignal West Dura ECL kit (InVitrogen, Slangerup, DK). Primary antibodies were rabbit monoclonal anti-cleaved Notch1 (Val1744 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5X105 cells were used as controls and incubated in the blocking buffer while 6X105 cells were incubated with anti-BrdU monoclonal antibody (1:100; MOBU-1 clone, B35128, Thermo Fisher Scientific) for 1 hr at RT ...
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bioRxiv - Immunology 2020Quote: ... They were then exposed at room temperature first for 1 h to primary antibodies in blocking buffer and then for 1 h to Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific) and Hoechst 33342 in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with the blocking buffer and re-suspended in PBS containing 5 mg/ml BSA (Thermo Fisher Scientific, USA) and 0.5 U/ml RNsing plus (Promega ...
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bioRxiv - Neuroscience 2021Quote: Primary neurons on cover glasses or 20-µm cryostat-sectioned coronal brain slices were incubated for 1 hour at room temperature with blocking buffer [PBS containing 5% (v/v) goat serum (#16210-064, Gibco, New Zealand), 1% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... tissues were wash 3 times with PBS and incubate with protein blocking solution that contain 0.5% Triton X-100 (Fisher Scientific, Hampton, NH). Then tissues were incubated in 1:250 diluted primary antibody (CD31 (PECAM-1 ...
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bioRxiv - Genetics 2020Quote: ... they were incubated for 1 hour at room temperature with blocking solution containing the secondary antibodies (Alexa fluor 568 Goat anti-rabbit (1:800; Molecular Probes, #A11011) Alexa Fluor 488 goat anti-mouse (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with blocking buffer made up with 2% bovine serum albumin (BSA) Fraction V (Thermo Fisher Scientific, Waltham, MA, USA), 1% gelatin from bovine skin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 2 h in blocking/permeabilization buffer including goat anti-rabbit AlexaFluor 568 or goat anti-mouse 488 (Invitrogen; 1:500). Sections were washed in 0.01 M PBS after primary and secondary antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution: Alexa 488-goat anti-rabbit (1:1000; Thermo Fisher Scientific) and Alexa 594-donkey anti-mouse (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After 72h of incubation the sections were incubated with secondary antibodies diluted in blocking solution for two hours: Alexa 594 anti-rabbit (Invitrogen, 1:1000), Alexa 488 High Cross Absorbed (Invitrogen ...