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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for Recombinant Mouse Ager His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: TNF-α released by mouse peritoneal macrophages was measured by a Mouse TNF-α ELISA Kit (Invitrogen, BMS607-3TEN) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher). First ...
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bioRxiv - Genomics 2022Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Genetics 2024Quote: ... followed by HRP-conjugated goat-anti mouse secondary antibodies at 1/20,000 dilution (Goat anti-mouse-HRP conjugate, cat# 31430, Thermofisher). The proteins were detected by SuperSignal chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Biochemistry 2021Quote: ... All RNA solutions were prepared by solubilizing in nuclease free water in the presence of RNAseOUT™ recombinant ribonuclease inhibitor (Thermo Fisher, Cat. #. 10777019) at a final concentration of 0.4 U/μL ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant proteins were produced in their respective expression systems according to standard procedures and purified using either Ni-NTA (ThermoFisher Scientific, Waltham, MA, USA) or Strep-Tactin (IBA Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then co-transfected with recombinant plasmid (300 ng) together with miR-544-3p mimic or negative control mimic (50 nM) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) and Lipofectamine RNAimax (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
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bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were developed with a mouse polyclonal anti-SmHDAC8 antibody (1/1000) or mouse polyclonal anti-SmRho1 antibody (1/1000) and peroxidase coupled anti-mouse secondary antibody (1/50 000; Invitrogen). Detection was carried out by chemiluminescence using SuperSignal™ West Dura Extended Duration Substrate (Thermo Scientific ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: Mouse forebrains fractions or HEK293T cell lysates were incubated with either mouse anti-CaMKIIα (Thermo Fisher Scientific catalog #MA1–048), rabbit anti-Shank3 (Bethyl catalog #A304-178A) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-Mcm3 antibody (M038-3; MBL) and anti-mouse IgG conjugated to Dynabeads M-280 (Thermo Fisher Scientific) were used as the primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Wells were consecutively washed two times for 5 min in washing buffer before incubation with secondary antibodies (Alexa Fluor-488 goat anti-mouse IgG, Invitrogen, A-11029; Alexa Fluor-647 goat anti-mouse IgG, Invitrogen, A-21236 ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488-conjugated goat anti-mouse-IgG antibodies and Alexa-Fluor-594-conjugated goat anti-mouse-IgG antibodies (Thermofisher) were used.
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bioRxiv - Microbiology 2020Quote: ... Samples were separated by SDS-PAGE and Western blotting carried out using a mouse mono- and polyubiquitinated ubiquitin conjugate (Ubiquigent) or mouse Ub-K63 (ThermoFisher) antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 hr at room temperature in the dark with secondary antibodies Alexa488-conjugated goat anti-mouse IgG and Alexa546-conjugated goat anti-mouse IgG (Invitrogen). Nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Additional labeling of C2C12 with MF-20 hybridoma mouse IgG2B primary antibody and Alexa Fluor 546 goat anti-mouse IgG2B secondary antibody (Invitrogen) was used to evaluate the cell differentiation into myotubes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the anti-BiP mouse monoclonal antibody and the anti-mouse secondary antibody were obtained from Invitrogen (Grand Island, NE, USA). Dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2022Quote: Cytokines in mouse lung homogenates were measured using a custom ProcartaPlexTM Mouse Mix & Match Panels kit (Invitrogen Waltham, MA, USA) on the Bio-Plex 200 (Bio-Rad Laboratories Ltd).
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bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa488- and 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa 488-/ 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec) and an AF647 conjugated donkey anti-mouse IgG antibody (Invitrogen). Following 3 washes with PBS containing 0.5% BSA ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... Secondary antibodies included goat anti-rabbit and goat anti-mouse from Novus conjugated to DyLight 650 or Janelia Fluor 646 and goat anti-mouse from ThermoFisher Scientific conjugated to Alexa Flour 488 for immunofluorescence ...
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bioRxiv - Physiology 2022Quote: ... Sections were then incubated with fluorescently conjugated secondary antibodies (Goat x Mouse IgM Alexa 350 and Goat x Mouse IgG Alexa 488, Invitrogen) and imaged using an inverted fluorescence microscope (Axio Observer ...
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bioRxiv - Immunology 2022Quote: ... the wells were incubated with PBS (control) or serially-diluted irrelevant mouse IgG (anti-green fluorescence protein of mouse IgG, ThermoFisher) or irrelevant rabbit IgG (anti-green fluorescence protein of rabbit IgG ...
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40, #124610, BioLegend; or PE anti-iNOS, #12592082, Invitrogen; or FITC anti-mouse CD8a ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies (Alexa fluor 546 goat anti-mouse IgG and Alexa fluor 488 goat anti-mouse IgG, Thermo Fisher Scientific) were incubated at 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: Larvae were dissected and stained as previously described23 with BRP (mouse BRPnc82, DSHB; diluted 1:200) and secondary Alexa Fluor 488-coupled goat anti-mouse (Invitrogen Cat#A-11001 ...
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bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse IgG (H+ L) Alexa Fluor 555 and Goat anti-Mouse/Rabbit IgG (H+L) FITC was purchased from Invitrogen; Mouse anti-Flag/HA-tags beads was purchased from Abmart ...
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bioRxiv - Microbiology 2019Quote: ... for 1 hour at room temperature followed by anti-mouse IgG labeled with Alexa Fluor 488 (anti-mouse IgG-Alexa Fluor 488; Invitrogen) secondary antibodies and 4′,6′-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2021Quote: ... For immunofluorescence analysis the cells were fixed as described above and stained with a monoclonal mouse antibody against the viral capsid proteins followed by staining with a goat anti-mouse IgG Alexa Fluor 488 (Invitrogen).