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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for Recombinant Mouse Ager His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 1:500 mouse anti-GFP (ThermoFisher, #A11120), 1:500 chicken anti-GFP (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: 488 labelled anti-mouse (Invitrogen, 1:500) and Alexa Fluor 546 labelled anti-rabbit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... mouse mAb α-TY1 (Invitrogen #MA5-23513), 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse CD8 (Fisher Scientific 50562192) mAb or isotype control (IP ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse 10 μg/mL laminin (Gibco). Primary astrocytes obtained from E18 rat cortices via 15 min trypsinisation (10 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse CD8 (Fisher Scientific 50562192) mAb or isotype control (IP ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mg/ml mouse laminin (Invitrogen 23017015), and 2 μg/ml doxycycline.
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bioRxiv - Neuroscience 2024Quote: ... mouse CYP7B1 (OTI1G7) 1:500 (TA807549, Thermofisher), rabbit HSD3B7 1:1000 (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mg/ml mouse laminin (Invitrogen, 23017015), and 2 μg/ml doxycycline) ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-Mouse IgG HRP-conjugated (ThermoFisher), goat anti-Mouse IgG Alexa Fluor 488-conjugated (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... The residual amount of endotoxin in the recombinant S100A4 preparation was examined using a Pierce™ Chromogenic Endotoxin Quant Kit (Thermo Fisher Scientific). Mice were anesthetized using isoflurane prior to immunization ...
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bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: The interaction of the NAB1 promoter fragments with recombinant LCRF-SBP (rLCRF-SBP) was analyzed by using the LightShift®Chemiluminescent EMSA Kit (Thermo Scientific™), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
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bioRxiv - Biochemistry 2020Quote: Recombinant bacmids containing the gene of interest (PDI) were generated according to the Baculovirus Expression system manual (Thermo Fisher Scientific, Cat. No. 10359016). In brief ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Microbiology 2022Quote: Cu-sensitive strains harboring the recombinant pTR plasmids were harvested for metal content determination by using inductively coupled plasma mass spectrometry (ICP-MS) (Thermo Fisher Scientific, USA). E ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The linearized recombinant plasmid was used as templates to synthesize capped RNAs (cRNAs) with the mMessage mMachine T7 Kit (Life Technologies, Carlsbad, CA). The cRNAs were dissolved in RNase-free water at a concentration of 1000 ng/μL ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Preprotech, cat#100- 18B) and 50 ng/mL recombinant sonic hedgehog (SHH, Preprotech, cat#100-45) in Knockout DMEM (Gibco cat #10829-018), 10% Knockout serum replacement (KOSR ...
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bioRxiv - Microbiology 2019Quote: ... and then stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Microbiology 2021Quote: ... ExpiSf9 cells for recombinant baculovirus production were cultured at 27°C and 125 rpm in ExpiSf CD Medium (Thermo Fisher Scientific, Waltham, MA). B19V-infected plasma sample was obtained from a donation center (CSL Behring AG ...
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bioRxiv - Biochemistry 2019Quote: ... A concentration range of indicated recombinant proteins was analyzed by thermal melt assays in SEC buffer using SYPRO Orange (Molecular Probes, cat.# S6651) using a Biorad CFX96 Touch Real-Time PCR C1000 System (ramping from 4 to 100°C at 1°C per minute).
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bioRxiv - Microbiology 2019Quote: ... and stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (lot: 917439A, Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... were delivered into H9 cells along with recombinant True Cut Cas9 v2 using the Neon Transfection system (Thermo Fisher Scientific, Cat. No MPK5000). H9 cells (1×105 cells ...
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in blocking buffer thrice and stained with 1:1000 dilution of Alpaca anti-Rabbit IgG Nano (VHH) Recombinant Secondary Antibody conjugated with Alexa Fluor™ 647 (Invitrogen SA5-10327), Goat anti-Mouse IgG conjugated with Alexa Fluor™ Plus 488 (5 μg/mL final concentration ...
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Genomics 2022Quote: ... The mini prep DNA was then used with the Bac-to-Bac™ expression system to generate a recombinant bacmid as per manufacturer conditions with MAX efficiency™ DH10Bac™ Competent Cells (ThermoFisher). After 4 hours of transformation recovery ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Microbiology 2023Quote: ... the binding of HIV+ plasma was detected using a biotin-tagged dimeric recombinant soluble FcγRIIIa (0.2 μg/mL) followed by the addition of Alexa Fluor 647-conjugated streptavidin (Thermo Fisher Scientific; 2 μg/mL). Infected cells were then stained intracellularly for HIV-1 p24 ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Biochemistry 2023Quote: ... The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific, Waltham, Massachusetts, USA). Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius ...
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bioRxiv - Cancer Biology 2022Quote: One million of BMDCs cultures in the absence or presence of 10 ng/ml of recombinant MDK (indicate provider and origin) were incubated with 1 μg/ml of Ovalbumin-AF594 conjugate (Thermo Fisher Scientific; O34781) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Genomics 2020Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... A total of 21,981 mouse genes were included in the Affymetrix Mouse Clariom S Assay (Affymetrix, Santa Carla, CA) and all steps were performed at the McGill University and Génome Québec Innovation Centre (Montréal ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with a mouse primary antibody (DSHB 1D4B) and anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific).