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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for Recombinant Human TNFSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Becton Dickinson) along with biotinylated PvAMA1 using Streptavidin coupled with allophycocyanin (Becton Dickinson) and SYTOX Green Dead Cell Stain (Invitrogen) to gate out dead cells ...
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bioRxiv - Immunology 2022Quote: Serum ELISAs were performed using randomly biotinylated SOSIP trimers using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Pathology 2023Quote: Vip3Aa toxin was biotinylated with a 1:30 (protein:biotin) molar ratio of EZ-Link Sulfo- NHS-LC-Biotin (Life Technologies) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vectastain Elite ABC kit ...
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bioRxiv - Synthetic Biology 2023Quote: ... a mixture of 3 GFP-specific biotinylated primers were used to capture GFP transcripts using Sera Mag Beads (Fisher Scientific). After a second round of DNase treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... using biotinylated RNA oligonucleotides (LGC biosearch technologies, Risskov, Denmark, sequences in supplements) immobilized on Dynabeads M-280 streptavidin magnetic beads (Invitrogen) and incubated with HeLa cell nuclear extract ...
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bioRxiv - Microbiology 2023Quote: ... The EdU-labeled RNAs were then biotinylated and captured by using the Click-it Nascent RNA Capture Kit (Life Technologies), in accordance with the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The GDF-15 and GFP antibodies were biotinylated using an EZ-Link Micro NHS-PEG4-Biotinylation Kit from Thermo Fisher Scientific (21955 ...
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bioRxiv - Biochemistry 2022Quote: ... Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher) at room temperature prior to use.
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were then biotinylated by incubation with 1 mg/ml of the cell membrane-impermeable reagent EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher) dissolved in PBS for 30 minutes at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vecstatin Elite ABC kit ...
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bioRxiv - Bioengineering 2023Quote: The binding of the scFvs to biotinylated rHl and rLr (30 nM) was assessed using a DELFIA-based assay on black Maxisorp plates (Nunc), as previously described 32 ...
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bioRxiv - Cell Biology 2023Quote: CEM WT and ADAM10 KO cells were biotinylated using the Pierce™ Cell Surface Biotinylation and Isolation Kit (Thermo Fisher). After biotinylation ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200, ThermoFisher Scientific A28181) antibody for two hours at room temperature and then a streptavidin conjugated Alexa-Fluor 488 (1:200 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the cells were incubated with biotinylated target, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at one-quarter of the concentration of the biotinylated targets ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were incubated with biotinylated Hsc70, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at 1/4 concentration of the biotinylated Hsc70 ...
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bioRxiv - Biochemistry 2023Quote: ... Hisα1-subCD3-WT (Legrand et al., 2020) was biotinylated with EZ-Link™ NHS-PEG4-Biotin (Thermo Scientific™ A39259) in a 1:1.5 ratio at 25°C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... at varying depths was made targeting the deep layers of the primary motor cortex using 10% biotinylated dextran amine (BDA; 10,000 MW) (Life Technologies) (n=4 ...
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bioRxiv - Immunology 2024Quote: Affinity purified SARS-CoV-2 (Wuhan) RBD was biotinylated using EZ-Link Sulfo-NHS-LC-biotin (Life Technologies, USA, A39257) accordingly to manufacturing protocol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The membranes were then treated with secondary antibodies (biotinylated goat anti-rabbit IgG (#65-6140, Invitrogen, Rockford, IL, United States), biotinylated goat anti-rat IgG (#405428 ...
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bioRxiv - Neuroscience 2024Quote: ... and Biotinylated Dextran Amine (BDA, cases AV, AT: equal parts 10% 10 kDA and 10% 3 kDA, 5 µl; Invitrogen). Dextran amines with molecular weight 10 kDA (BDA in AT and AV ...
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bioRxiv - Neuroscience 2024Quote: ... mice received an injection of 2 µL biotinylated dextran amine (BDA, 0.2 g/ml, Thermo Fisher Scientific, D1956, Waltham, MA) diluted in PBS at the cervical level two days prior to euthanasia ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed extensively in TBS before being incubated with biotinylated goat anti-rabbit IgG (H+L) secondary antibody (ThermoFisher Cat# B-2770 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We used tracer-coated insect needles to place a small BDA-crystal (biotinylated dextran amine, 3kDa; Invitrogen, Eugene, OR, USA) onto the proximal end of the severed nerve and incubated the preparation for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... Preps for use in microtubule nucleation assays were biotinylated by incubation with 40 µM EZ-Link NHS-PEG4-Biotin for 1 hour (ThermoFisher). γ-TuNA/γ-TuRC was eluted by protease cleavage of the HRV3C site with PreScission overnight ...
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bioRxiv - Bioengineering 2024Quote: ... GFP (Appendix B (Scholz et al., 2000)) was biotinylated using EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, cat. no. A39259) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... or RSV antigen mix were biotinylated with EZ-Link Sulfo-NHS-LC-Biotin following manufacturer’s instructions and coupled to FluoSpheres NeutrAvidin beads (Molecular Probes) (16 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Thymocyte suspensions were labeled with biotinylated CD4 and CD8 antibodies incubated with MACS Streptavidin Microbeads (Miltenyi, Biotec) in HBH buffer (HBSS (Gibco), 0.5% BSA (Sigma-Aldrich) ...
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bioRxiv - Genetics 2024Quote: ... Similar to SNP-seq, 500 ng of different alleles (31 bp, biotinylated) from a SNPs were attached to 25 μl streptavidin-Dynabeads (Invitrogen) and then incubated with 100 μg of Daudi nuclear extract for 1 h with or without non-biotinylated competitor (the same alleles) ...
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bioRxiv - Biophysics 2019Quote: ... N-terminal-tagged MBP-TRPA1 under the control of a CMV promoter was expressed in HEK293F cells using the BacMam system (Thermo Fisher). Protein expression was induced at cell density 1.5-2.5 × 106 cells ml- with media supplemented with 5 mM sodium butyrate and 5 µM ruthenium red ...
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3x in PBS with 2% FBS and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-mouse IgG) and fluorescently-tagged phalloidin (Molecular Probes), in a humidity chamber for 1hr ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T cells were grown in 10 cm dishes and transfected with APEX2-tagged mammalian expression plasmids using Lipofectamine 3000 (Thermo Fisher). Cells were washed off the plate 12-15 hours post-transfection with Dulbecco’s PBS and then pelleted at 500x g ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa Kyoto cells were transfected with two guide RNA and one HDR donor plasmid (or 4 guide RNA and two HDR donor plasmids for the EGFP-STAG1 FLAG-mRFP-STAG2 double tagged cell line) using Lipofectamine 2000 (Invitrogen 11668019). Cells were grown for 7-10 days before sorting single cells into 96 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... RING1Bfl/fl conditional mESCs were transfected with pCAG vectors encoding 2xFlag-HA-tagged mouse wild-type or RING1B I53A/S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: N-terminally MBP-tagged (pTHMT) FUS CHOP fusion type II and CHOP oncogene were expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7–0.9 before induction with 1 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Microbiology 2021Quote: ... A549 cells were transiently co-transfected with 100 ng in total of an N- or C-terminal LgBiT and SmBiT tagged construct using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher). All possible combinations of the N-terminal and C-terminal-tagged split luciferase protein pairs were tested ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Neuroscience 2019Quote: pLVX-EF1a-TS-EGFP-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged EGFP cDNA (DNA String byGeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Neuroscience 2019Quote: ... pLVX-EF1a-TS-OPT-FUS-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged codon optimized FUS cDNA (Gene synthesis by GeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... along with 50 ng and 100 ng of expression plasmids encoding 3xHA-tagged wildtype or mutant mouse PORCN-D as indicated using Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissues were counterstained with wheat germ agglutinin (WGA) tagged with Alexa Fluor™ 647 (#A-31573, ThermoFisher Scientific, Waltham, MA) and DAPI (#H-1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... cells were rinsed thrice with PBS and incubated with secondary antibody (donkey anti-goat IgG, tagged with Alexa Fluor 488, Thermo Fisher) at 1:500 dilution for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected with 100ng EGFP to mark cell bodies and 50-100ng of GFP tagged TDP-43 constructs using Lipofectamine 2000 (Invitrogen 52887), as previously described[41 ...