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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Recombinant Human TNFSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Both Fn39-10 S1417C mutant and its inactive version were biotinylated with Maleimide-PEG11-Biotin at residue 1417 (ThermoFisher Scientific) in PBS.
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bioRxiv - Developmental Biology 2020Quote: ... and biotinylated EMSA probes and unlabeled competitor probes were synthesized by using a LightShift® Chemiluminescent EMSA Kit (Thermo Scientific). The daf-7p-WT-CRE and daf-7p-mut-CRE probes with the following sequences were employed:
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bioRxiv - Immunology 2022Quote: ... CCL2 was affinity purified from the in vitro reactions using Phytip streptavidin coated tips (Phynexus) bound to biotinylated anti-CCL2 antibody (Invitrogen) using the Phytip protocol ...
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bioRxiv - Immunology 2022Quote: All antigens were biotinylated using an NHS-Sulfo-LC-LC kit according to the manufacturer’s instruction (Thermo Fisher, MA, USA) if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff ...
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bioRxiv - Genomics 2020Quote: ... Biotinylation efficiency was assessed by running an aliquot of the biotinylated oligo alongside unbiotinylated oligo of the same sequence and 10 bp DNA ladder (ThermoFisher) on a 20% TBE-Urea PAGE gel (SequaGel ...
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bioRxiv - Microbiology 2021Quote: ... The biotinylated cDNA probe for each segment was mixed with the Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific, Waltham, MA) and incubated on a rotating wheel at 37°C for 1 h in a LiCl hybridization buffer (10 mM Tris-HCl [pH 7.9] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hybridization and detection of biotinylated probes was performed using the North2South™ Chemiluminescent Hybridization and Detection Kit (Thermo Fisher Scientific) following manufacturer instructions.
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bioRxiv - Bioengineering 2019Quote: ... These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific). Excessive biotin was removed by buffer-exchange into PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The mRNA concentrations and integrity were determined with Agilent’s NanoDrop instrument then amplified and biotinylated with the Affymetrix GeneChip 3’ IVT PLUS Reagent kit (ThermoFisher Scientific) following manufactirer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... and the biotinylated nascent transcripts were isolated in a total of three rounds of streptavidin-coated magnetic bead (M-280, Invitrogen) purifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated cDNAs were prepared from a minimal 100ng of total RNA using Life Technologies WT-plus RNA Amplification system (Ambion). Following the amplification ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo scientific) or fluorescent secondary antibody to visualize proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated RNA baits were used in a ratio 1:12 to Hi-C libraries (25 ng biotinylated RNA baits per 300 ng of Hi-C library) and supplemented with 30 units SUPERase-In (Ambion). Biotinylated RNA baits for capture DNA-Seq were used in a ratio of 1:3.33 (300 ng RNA baits per 1,000 ng genomic DNA library ...
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bioRxiv - Genomics 2020Quote: ... The DNA was eluted in 150 μL 10 mM Tris-HCl pH 8 and the biotinylated molecules captured with Dynabeads M280 (Invitrogen) according to the manufacturer’s protocol but using150 μL Bead Binding buffer (Phase Genomics ...
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bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and HAΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated with a 20-fold excess of 10 mM EZ-Link NHS-LC-biotin (ThermoFisher) and unbound biotin was removed using a Zeba Spin Desalting Column (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked and hybridized overnight with 100 ng/mL of 5′ biotinylated DNA probe (Table S3) in ULTRAhyb (Ambion) hybridization buffer at 42°C ...
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bioRxiv - Microbiology 2020Quote: ... TUNEL-labeled DNA was detected using a biotinylated anti-BrdU antibody and streptavidin-Alexa Fluor 594 conjugate (ThermoFisher Scientific, Australia). At least 100 infected cells were counted on each slide ...
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bioRxiv - Cell Biology 2021Quote: ... a 3-step approach was taken using a biotinylated secondary antibody and a final incubation step with a strepatividin-linked AlexaFluor antibody (Invitrogen). Slides were inserted into a 1 μg/ml solution of 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2020Quote: ... sodium azide and endotoxin were removed from the biotinylated antibodies with Zeba desalting spin columns and High-Capacity Endotoxin Removal spin columns (ThermoFisher) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Biotinylated SARS-CoV-2 prefusion stabilized S trimer was incubated with yellow-green streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo Scientific) or fluorescent secondary antibodies with species-specific Alexa Fluor-488 or -568 (Invitrogen A-11039 or A-11011 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were purified by phenol/chloroform extraction and RNA precipitation (total RNA) or MyOne Streptavidin C1 beads (biotinylated probes; Invitrogen). For total RNA purification ...
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bioRxiv - Developmental Biology 2022Quote: Biotinylated bait ectodomain transfections were dialysed against PBS using SnakeSkin dialysis tubing (molecular weight cut-off 10,000 Da; Thermo Scientific) and several buffer changes (approximately 25 – 30 L in total) ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were hybridized at 42 °C with 5 nM of a 5’-biotinylated oligonucleotide probe (Table S4) in ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) and then washed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Transferred RNA was UV cross-linked and hybridized overnight with 100 ng/mL of 5’-biotinylated probes (Table S2) in ULTRAhyb hybridization buffer (Ambion) at 42 °C as described previously (27 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were incubated with biotinylated RBD, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidinphycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at ¼ concentration of the biotinylated RBD ...
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bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μl pre-mix (25 μl 0.4 mM Biotin-dATP (Invitrogen, #19524016), 25 μl 0.4 mM Biotin-dCTP (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Amplified and biotinylated sense-strand DNA targets were generated from total RNA using the WT Plus Kit (Thermo Fisher, MA). Fragmented and biotinylated sense-strand DNA target (5.5 µg ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... 300 μg of purified monoclonal antibodies were chemically biotinylated using a 20-fold molar excess of sulfo-NHS-biotin (ThermoFisher) for two hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2019Quote: ... mucins were also detected using biotinylated Ulex europaeus agglutinin I (UEA-I) lectin to detect fucose residues (Vector, Burlingame, CA) or with biotinylated maleimide to alkylate and detect reduced sulfhydryls (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biochemistry 2021Quote: ... briefly sonicated chromatin materials diluted using hybridization buffer and hybridized with 100pmol of biotinylated DNA probes targeting HOTTIP or LacZ containing 100 mL of Streptavidin-magnetic C1 beads (Invitrogen). RNA and DNA hybrids and RNA binding proteins were subjected to analysis by qRT-PCR and western blot with respective antibodies respectively ...
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bioRxiv - Biochemistry 2021Quote: ... that were hydrated for 10 min in assay buffer then plunged in a solution containing 1 μM 3′ end–biotinylated TAR RNA in assay buffer with 1x RNAase inhibitor (RNA Secure, Invitrogen) and 0.1 μM BSA for the RNA loading step ...
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bioRxiv - Biophysics 2021Quote: ... Purification was carried out exactly as for the non-biotinylated constructs and biotinylation was confirmed by western blot using NeutrAvidin-HRP (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... DNA was then recovered and sonicated to a length between 150 and 200 bp and biotinylated DNA fragments were purified using streptavidin beads (MyOne C1, Invitrogen). The DNA was then end-repaired and ligated to hairpin adaptor BU2 and amplified by PCR ...
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bioRxiv - Immunology 2021Quote: Immunoprecipitations for competition assays were performed with 8 μg of C-terminally biotinylated nanobody on M-280 streptavidin magnetic beads (Dynabeads, Invitrogen) and 10 μg of spike or RBD preincubated with 10 μg of the indicated (HIS-tagged ...
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bioRxiv - Immunology 2020Quote: ... Each well was washed and binding of biotinylated monoclonal antibodies was determined with a 1:30000 dilution of HRP-conjugated streptavidin (ThermoFisher). HRP was detected with TMB and stopped with 1% HCl ...
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bioRxiv - Biochemistry 2022Quote: ... About 1 pmol per sample of the biotinylated template DNA was captured on the streptavidin magnetic beads (Dinabeads MyOne streptavidin C1, Invitrogen) in buffer A (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated RNAs were pre-coupled to Nucleic Acid Compatible Streptavidin Magnetic Beads in RNA Capture Buffer (Pierce/Thermo Fisher Scientific) for 3h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The excess biotin of biotinylated mAbs was removed with 7k molecular weight-cutoff (MWCO) Zeba spin desalting columns (Thermo Scientific). Plates were coated with 2 μg/ml RBD antigen overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... 2×105 CD11b+ monocytic BM cells that were isolated by biotinylated anti-CD11b (1:100; clone M1/70; ThermoFisher Scientific) and anti-biotin microbeads (Miltenyi Biotec) ...
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bioRxiv - Systems Biology 2024Quote: ... and 200 cycles per burst for 65 seconds) and biotinylated DNA fragments were pulled down using MyOne Streptavidin T1 beads (Invitrogen). End repair ...
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bioRxiv - Neuroscience 2024Quote: ... were injected with 0.2 µl of the anterograde axonal tracer biotinylated dextran amine (BDA, molecular weight 10 000, 0.1 µg/µl; Molecular Probes) at 0.2 µl/min into the ipsilesional stroked cortex at ...
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bioRxiv - Immunology 2024Quote: ... Becton Dickinson) along with biotinylated PvAMA1 using Streptavidin coupled with allophycocyanin (Becton Dickinson) and SYTOX Green Dead Cell Stain (Invitrogen) to gate out dead cells ...