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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were quantified using a colorimetric peptide assay (Thermo Fisher Scientifc). Peptides (1 µg ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using colourimetric peptide assay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using a colorimetric peptide assay (ThermoFisher Scientific). All samples were reconstituted in loading buffer (5% CH3CN ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were injected and eluted through a 50 cm EASY-Spray column PepMap RSLC C18 (Cat. ES803; Thermo Fisher Scientific) with a 135-min gradient at constant 300 nL/min kept at 40°C ...
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bioRxiv - Systems Biology 2020Quote: ... Desalted peptides were reconstituted in 50 mM HEPES (pH 8.5) and labelled with TMT10plex158 Isobaric Label Reagent (Thermo Fisher Scientific) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The chromatographic separation of the peptides was achieved using a 50 cm long C18 Easy spray column (Thermo Scientific,US) at 55°C ...
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bioRxiv - Cell Biology 2021Quote: Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50-cm by 75-μm ID; ThermoFisher Scientific). The 125-min elution gradient at a constant flow rate of 300 nl/min was set to 5% phase B (0.1% formic acid in 99.9% acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a PepmapTM C18 reversed phase column (3 μm, 100 Å, 75 μm × 50 cm) (Thermo Scientific). Samples were fractionated with mobile phase A consisting of 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were further separated onto an Easy-Spray RSLC C18 column (75 µm i.d., 50 cm length, ThermoFisher Scientific) using a 30 min linear gradient (15% to 35% solvent B (0.1% formic acid in acetonitrile) ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50 cm × 75 μm ID; Thermo Fisher Scientific). Using a constant flow rate of 300 nL/min ...
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bioRxiv - Bioengineering 2024Quote: ... quenchbodies were eluted from the beads using 50 μL of 1.5 mg/mL Pierce™ 3 x DYKDDDDK Peptide (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: On day 2 of cardiac differentiation cells were dissociated using 0.5% EDTA and put into blocking buffer of 50% fetal bovine serum (FBS) in Dulbecco’s Modified Eagle Medium (DMEM)/F12 (ThermoFisher, Cat.#11320033). Cells were then pelleted and resuspended in 10% FBS in DMEM media ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was washed in blocking solution and treated with secondary antibody (anti-guinea pig 488 1:50 and anti-chicken 594 1:300, Invitrogen, WA316328) for 2-3 days ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were removed and spiked with 50 mM N-ethylmalamide before being added to 50 μL of magnetic protein G Dynabeads (Invitrogen) pre-conjugated with 10 μg mouse HA antibody (in house ...
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bioRxiv - Microbiology 2023Quote: ... were biotinylated by mixing 50 μM of protein with 50 μM of EZ-Link™ Sulfo-NHS-Biotin (Thermofisher Scientific) followed by incubation on ice for 2 hours ...
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bioRxiv - Biophysics 2021Quote: ... blocking of endogenous biotin was performed using a commercial blocking kit (Invitrogen, E21390). Cells were incubated in component A for 45 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... After blocking with 10% goat serum or Superblock blocking buffer (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed by incubating in trypsin (1 µg trypsin/50 µg protein; Thermo Fisher Scientific, Waltham, MA) for 3 h ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of desalted peptides were labelled immediately after being quantified with Qubit protein assay (Thermo Fisher Scientific). Multiplexed TMT samples were then fractionated using high-pH reverse phase chromatography ...
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bioRxiv - Cancer Biology 2021Quote: ... Lyophilized peptide aliquots of 400 µg (of starting protein) were labeled with TMT10-plex labeling kits (Thermo Fisher). Phosphopeptides were enriched with a 2-step enrichment process consisting of immunoprecipitation (IP ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested samples were purified using High Protein and Peptide Recovery Detergent Removal Spin Column (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein and peptide concentrations were determined by direct measurement on a Nanodrop 1000 UV-Vis spectrophotometer (Thermo Scientific), using peaks around 280 and 230 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... IPs were washed 8X with NET2 and bound proteins eluted with 3XFLAG peptides and extracted with Trizol (Thermofisher). Eluted RNA was reverse transcribed and amplified with spliceosomal RNA specific primers (Supplemental Table 4)
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bioRxiv - Immunology 2021Quote: ... Peptides were further purified from larger protein components by preparative HPLC on an Ultimate 3000 system (Fisher Scientific). Material was separated on a ProSwift RP-1S 4.6 x 50 mm column (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The peptide concentration was estimated using a BCA protein assay reagent kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were digested overnight (16 h) at 37°C and peptides were dried using a Speedvac (Thermo Scientific). Peptides were desalted with stage tips using the following procedure ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptide concentrations were determined with the PierceTM BCA Protein Assay Kit according to the manufacturer’s instructions (#23225, ThermoFisher). Samples were measured on an Orbitrap Q-Exactive plus (Thermo ScientificTM ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
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bioRxiv - Bioengineering 2023Quote: ... The purified peptide solution was dried and quantified using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). An equal amount of peptide (∼50 μg ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of desalted peptides were labelled immediately after being quantified with Qubit protein assay (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The TMT-set was further purified using High Protein and Peptide Recovery Detergent Removal Spin Column (88305, ThermoFisher) and Pierce peptide desalting spin columns (89852 ...
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bioRxiv - Microbiology 2023Quote: ... non-ribosome protected RNA was isolated from the cleared lysate (Trizol) and fractionated to a comparable size range by zinc-mediated fragmentation (Invitrogen) prior to gel purification.
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... the membranes were probed with anti-TasA antibody (rabbit) used at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (ThermoFisher). For immunodtection of FloT-YFP ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed once and blocked for 45 minutes in 0.5X protein-free blocking buffer (Thermo Fisher Scientific, USA) with 0.2 mg/ml dextran sulfate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoblot membrane was blocked with Pierce Protein-Free T20 (TBS) blocking buffer (product no. 37571, Thermo Fisher Scientific) and incubated with the first antibody indicated in the blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed once and blocked for 45 minutes in 0.5X protein-free blocking buffer (Thermo Fisher Scientific, USA) with 0.2 mg/ml dextran sulfate (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were directly subjected to background blocking using the Ultra Vision Protein Block for 5 min (ThermoFisher Scientific) and exposed to primary and secondary antibodies for 45 min and 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... Peptide digest assay standard (PierceTM Quantitative Colorimetric Peptide Assay, Thermo Scientific, USA) was as standard for quantitative analysis of peptides ...
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bioRxiv - Cell Biology 2022Quote: ... peptide concentration was measured using a colorimetric peptide quantification assay (Thermo Scientific). Finally ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were quantified using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions on a Varioskan LUX Multimode Microplate Reader and SkanIt 5.0 software (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptide concentration was determined by a quantitative colorimetric peptide assay (Thermo Fisher), and 1 μg aliquots of clean peptides from each sample were injected sequentially over a nano-flow C18 column (EasySpray ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide concentrations were determined using Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... peptides were desalted on PierceTM Peptide Desalting Spin Columns (Thermo Fisher Scientific) and concentrated in a SpeedVac concentrator.
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bioRxiv - Microbiology 2021Quote: ... The peptide digests were then cleaned up using peptide desalting columns (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were separated on an EASY-Spray™ Acclaim PepMap C18 column (50 cm × 75 μm inner diameter, Thermo Fisher Scientific) using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A ...
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bioRxiv - Immunology 2019Quote: ... The resulting peptides were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 1% acetic acid/acetonitrile gradient at 300 ml/min and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific ...