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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Peptide separation was carried out using an Acclaim PepMap RSLC C18 column (75 μm × 50 cm; Thermo Fisher Scientific) using standard reverse-phase gradients ...
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bioRxiv - Biochemistry 2024Quote: ... 75 µm x 50 cm) and peptides then analysed using an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific). Solvent A comprised 0.1% formic acid (v/v ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of an isolation buffer (50 mM ammonium bicarbonate, 1% SDS, 1x Protein Inhibitor Thermo Fisher Scientific, #78430) were added to each tube containing the pellet ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Biochemistry 2021Quote: Hela cell tryptic peptides were obtained from the commercially prepared Pierce HeLa Protein Digest Standard (Thermo Fisher). Each vial was reconstituted in 2% acetonitrile (ACN)/1% formic acid (FA ...
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bioRxiv - Plant Biology 2023Quote: ... Eluates were combined and peptide content was estimated using protein A280 on a NanoDrop 2000 (Thermo Scientific). Eluate was split into two fractions with the smaller fraction (∼5µg of peptide ...
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bioRxiv - Biophysics 2023Quote: ... The sample was purified using High Protein and Peptide Recovery Detergent Removal Spin Column (Thermo Fisher Scientific) and Pierce peptide desalting spin columns (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2021Quote: Soluble proteins (10 to 50 μg) were resolved on NuPAGE 4-12% Bis-Tris Protein Gels (Life Technologies) or on SDS-PAGE gels (12 or 15% ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear extracts were pre-cleared with 50 μL protein G conjugated superparamagnetic beads (Dynabeads Protein G, ThermoFisher Scientific) for 30 min at 40C ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Genetics 2021Quote: ... bound to 50 μL/sample (or 1.5 mg/sample) protein G-coated magnetic beads (Dynabeads Protein G, Invitrogen). The subsequent steps of immunoprecipitation and washes were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts containing equal amounts of protein (50 μg) were solubilized in 1xLDS Sample Buffer (Thermo Fisher, NP0007) and separated by sodium dodecyl sulfate/ polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2020Quote: ... Digestion products were resolved in 3-8% tris-acetate SDS-PAGE followed and visualized by Zinc staining (Life Technologies). Images acquisition (Chemi-Doc Camera ...
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bioRxiv - Microbiology 2022Quote: ... The GI tract and internal organs were fixed in zinc-buffered formalin (Z-FIX, Thermo Fisher Scientific, Waltham, MA) for histopathologic assessment.
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bioRxiv - Molecular Biology 2020Quote: ... except for a shorter blocking step of magnetic beads used for immunoprecipitation: Dynabeads™ Protein A (Invitrogen™) were blocked by washing three times for 5 minutes each ...
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bioRxiv - Cell Biology 2021Quote: ... For detection of ubiquitylated proteins, slides were first blocked with antibody blocking buffer (PBS, 0.1% Synthetic Block (Invitrogen)) for 10 min followed by 1.5 hours incubation at 4°C with anti-ubiquitin chain antibody FK2 (1 µg/ml FK2 in antibody blocking buffer) ...
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bioRxiv - Bioengineering 2019Quote: ... tissues were wash 3 times with PBS and incubate with protein blocking solution (Thermo Fisher Scientific, Waltham, MA). Then tissues were incubated in 1:150 diluted PSMA primary antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... non-function blocking β1-integrin antibody (K20) was labelled using an Alexa Fluor 488 protein labelling kit (Invitrogen). Cells plated in the CSMA were then incubated live with the K20-Alexa Fluor 488 conjugated antibody for 1 h on ice to allow labelling of cell-surface integrins ...
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bioRxiv - Genomics 2022Quote: PVDF membranes containing transferred proteins were incubated in blocking buffer (1 x TBS-T (Thermo Fisher Scientific, 28360), 5% Blotting-Grade Blocker (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... After the addition of 50 µL Dynabeads protein-A (Life Technologies, 10002A), the mixture was incubated at 4 °C for another 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 48 well plates were coated with 50 µg/ml protein G (Invitrogen) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... followed by addition of 50 μl of Protein A agarose beads (Invitrogen) pre-equilibrated in the lysis buffer and additional incubation for 1 h at room temperature on a rocker ...
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bioRxiv - Molecular Biology 2019Quote: ... 2×106 cells and 50 µg of Protein A Dynabeads (Life Technologies) were used per IP ...
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bioRxiv - Neuroscience 2021Quote: ... and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific) pre-washed with KHM buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μl of paramagnetic protein G beads (Dynabeads, ThermoFisher Scientific, Waltham, MA) were washed 3 times in 0.5% BSA in PBS for 15 minutes on ice and then 3 times in PBS for 5-minute washes on ice prior to addition of LP2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins (50 μg) were resolved on 4-12% NuPage polyacrylamide gels (Invitrogen) and transferred onto enhanced chemiluminescence (ECL ...
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bioRxiv - Systems Biology 2021Quote: ... we resuspended an aliquot of 50 microliters of protein G dynabeads (Invitrogen) and equilibrated the protein G beads with 1x IP buffer lacking protease inhibitors ...
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bioRxiv - Cancer Biology 2022Quote: ... Sonicated materials were pre-cleared with 50% protein A agarose (ThermoFisher 20421). Antibodies were added into pre-cleared material and rotated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... and 50 μl Pierce protein G magnetic beads (88848, Thermo Fisher Scientific) were used and to immunoprecipitate Myc-Ku80 ...
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bioRxiv - Immunology 2020Quote: 0.2 mg of purified protein in 50 µl PBS pH 7.4 (Gibco) was mixed with 15 µl of 20 times diluted SYPRO orange fluorescent dye (5000 x stock ...
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bioRxiv - Cancer Biology 2019Quote: ... 50 µl of protein A/G magnetic beads (88802, Thermo Scientific Fisher) were washed 3 times at room temperature with the co-IP buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were pre-coupled to 50 μL Dynabeads Protein A (Invitrogen, 10001D) in PBS containing 0.02% Tween and incubated at 21°C for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... using 50 μl of Dynabeads – Protein A (Novex 10008D; Life Technologies, USA) and anti-GFP (632381 ...
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bioRxiv - Molecular Biology 2022Quote: 0.2 mg of purified protein in 50 μl PBS pH 7.4 (Gibco) was mixed with 15 μl of 20 times diluted SYPRO orange fluorescent dye (5000 x stock ...
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bioRxiv - Cell Biology 2023Quote: ... a 50 µl bed volume of protein A Dynabeads (Thermo Fisher, #10001D) was re-suspened in 1 ml of FA Buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: 50 to 86 uL Dynabeads Protein-G beads (10004D, Thermo Fisher Scientific) were washed twice with PBS-T (0.02% Tween-20 in 1x PBS) ...
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bioRxiv - Cancer Biology 2023Quote: ... were bound to 50 μl of Dynabeads Protein G (Thermo Fisher, #10004D), previously washed with 0.5% BSA in PBS and rotated end-to-end overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μl Pierce Protein A/G Magnetic Beads (88802; Thermo Fisher Scientific) was added to each supernatant/antibody mixture and incubated for 2 hours at room temperature with rotation ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The extract was pre-cleared with 50 µl protein A Dynabeads (Thermofisher) per sample by end-over-end rotation for 1 hour at 4 °C followed by magnetic removal of the beads ...
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bioRxiv - Plant Biology 2024Quote: ... The remaining 900ul protein lysate was incubated with 50 μl Dynabeads (Invitrogen) equilibrated with anti-GFP or anti-cMyc antibody at 4°C for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 µL of magnetic Dynabeads coupled to G protein (Invitrogen, Fisher Scientific) were coupled to anti-Flag M2 antibody (F1804 ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 µL of magnetic Dynabeads coupled to G protein (Invitrogen, Fisher Scientific) were coupled to anti-Flag M2 antibody (F1804 ...
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bioRxiv - Microbiology 2022Quote: ... Labelled peptides were quantified (Pierce Colorimetric Peptide Assay Kit, Thermo Scientific) and mixed 1:1:1 based on peptide amount.