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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: αT-catenin protein fragments were incubated with or without 1 mM BS3 (Thermo Scientific) in 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation of Cav-1 was performed using Dynabeads™ Protein G Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: THP-1 cells were lysed in M-PER mammalian protein extraction reagent (Thermo Scientific) supplemented with Pierce protease inhibitor (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody is Rabbit polyclonal antibodies against green fluorescent protein (GFP) (1:500, Invitrogen). Secondary antibodies were conjugated with AlexaFluor-488 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Genetics 2019Quote: ... with the anti-HRPK-1 antibody covalently crosslinked to the Protein A Dynabeads (ThermoFisher) using dimethylpimelimidate1 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-green fluorescent protein (GFP) at 1:000 (RRID: AB_221568, Invitrogen, Carlsbad, CA). Rabbit anti-leucokinin (Nässel et al. ...
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bioRxiv - Immunology 2019Quote: ... 1× protein transport inhibitor cocktail (PTIC) containing Brefeldin A/Monensin (eBioscience, Thermo Fisher Scientific) with or without anti-CD107a (eBio1D4B ...
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was expressed in sf21 insect cells using the vector pFastBac 1 (Invitrogen). One litre of cells (2.5×106 cells ml−1 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were washed and incubated with horseradish peroxidase-coupled protein G (1:1,000; Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl Dynabeads Protein A (Invitrogen 10001D) coated with 10 µg GINS antibodies for 2 h at 4°C with rotation ...
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bioRxiv - Immunology 2022Quote: ... at a protein/trypsin ratio of 1:50 and 10 % acetonitrile (Thermo Fisher Scientific), in a final volume of 100 μl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 mg of total protein was resuspended in 100 μl NativePAGE sample buffer (Invitrogen), lysed with 1.5% digitonin (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-glial fibrillary acidic protein (GFAP) antibody (Thermofisher, Cat# 13-0300, 1:70), and chicken anti-microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2023Quote: ... All other proteins were detected with secondary antibodies conjugated to DyLight800 (1:10000, ThermoFisher). Images were acquired with a ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used: RhoA (Invitrogen, #PA5-87403, 1:500), Rac1 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: The extracts (1 mg protein) were pre-cleared with control 4%-Agarose resin (ThermoFisher) for 1h at 4 °C with constant rotation ...
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bioRxiv - Pathology 2024Quote: ... The protein mixture was separated on a 4-12% NuPAGE gel (1 mm, Invitrogen). Samples were run at room temperature for 35 min at 200 V ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected in localization experiments using Streptavidin-Alexa674 (Life Technologies, 1:1200).
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bioRxiv - Genomics 2023Quote: 1 μg of antibody was coupled with 10 μL protein A (Invitrogen, cat# 10002D) and 10 μL protein G (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and Chlamydia heat-shock protein 60 (cHsp60) (ThermoFisher Scientific MA3-023, 1:2000 dilution), followed by secondary antibodies AlexaFluor 488 anti-mouse (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/10 diluted lysates were quantified using the Pierce BCA protein assay (Thermo Scientific). 30 μg of protein lysates were mixed with equal parts 2x Loading buffer (21% w/v glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... TDP-43 protein clusters were visualised using anti-mouse AlexaFluor488 antibody (1:2000, ThermoFisher), incubated for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented 1:2000 with Neuron Growth Factor (Ngf 2.5S Native Mouse Protein, Invitrogen) in the right ...
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bioRxiv - Cell Biology 2024Quote: ... was used to stain proteins and SYTOX™ Deep Red (1 µM, ThermoFisher S11380) was used as a DNA stain.
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... The surviving clones were screened for expression of the desired proteins in the presence of 1 μg ml−1 doxycycline (Invitrogen). Expression of Myc-Cdk11 was also induced with 1 μg ml−1 doxycycline in the subsequent experiments.
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Bioengineering 2021Quote: ... Immunostaining of BBB constructs for tight junction protein ZO-1 was conducted using ZO-1 mouse monoclonal antibody conjugated with Alexa 488 (ThermoFisher). Hydrogels were removed from the transwell insert using a biopsy punch ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP and SYPRO orange protein gel stain at a 1:500 dilution of the manufacturer’s stock (Invitrogen, S6651). The samples also contained 5 μM NBD protein ...
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bioRxiv - Pathology 2020Quote: ... 1 mM EDTA and 1% SDS) and the protein concentration was quantified by the BCA assay (Thermo Fisher Scientific, #23225). Equal amount of whole cell extracts were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin-antibodies complexes were recovered by incubation with 30 μl of Dynabeads® Protein A/G 1:1 mix (ThermoFisher) for 2 h at 4°C under rotation ...
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bioRxiv - Genomics 2023Quote: ... Sonicated samples were pre-cleared to remove background signal through incubation with a 1:1 mix of Protein A/G Dynabeads (InVitrogen). Antibody was added at a concentration of 1 µg/µL per sample and immunoprecipitated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... Any bound proteins were eluted by adding a 1:1 mix of 10 mM TBS and LDS buffer (Life Technologies) to the first well and then transferring from well to well ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2021Quote: ... a 1 mg/mL solution of these proteins was mixed with the Protein Thermal Shift™ Dye kit (Thermo Fisher, Waltham, MA, USA; Cat # 4461146) in triplicate as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.
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bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...