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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Magnetic protein G Dynabeads (30 mg ml−1; Invitrogen, 10004D) were coated with anti-c-Myc 9E10 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... stained for 1 hour with Imperial Protein Stain (Thermo Scientific), rinsed several times ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti-Green Fluorescent Protein (GFP; 1:1000; Invitrogen; A6455), Chicken anti-Green Fluorescent Protein (GFP ...
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bioRxiv - Cell Biology 2023Quote: ... or total Syk or SHIP-1 proteins (Thermo Fisher Scientific). We identified BCR-Fab’ clusters as described ...
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bioRxiv - Molecular Biology 2023Quote: ... visinin-like protein 1 primary antibody (Thermo Scientific Pierce Products) diluted 1:100 ...
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bioRxiv - Immunology 2024Quote: ... To enrich for MCL-1 binding protein immunoprecipitation was preformed using the Dynabeads Protein G IP Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The 1:1 mixture of Novex Sharp pre-stained protein standard (LC5800, Thermo Fisher Scientific) and MagicMark XP Western protein standard (LC5602 ...
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bioRxiv - Systems Biology 2023Quote: ... Total proteins were isolated in RIPA buffer containing 1% protease and 1% phosphatase inhibitors (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was evaluated and 1 mg total protein was incubated with 30 μl streptavidin-magnetic beads (ThermoFisher, 88816) for 1 h at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... An equal amount (500µg) of each protein lysate was precleared for 1 hour with magnetic Protein G Dynabeads (Invitrogen) and immunopurified with 40µl washed anti-V5 agarose beads (Sigma ...
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed by incubating in trypsin (1 µg trypsin/50 µg protein; Thermo Fisher Scientific, Waltham, MA) for 3 h ...
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bioRxiv - Genetics 2023Quote: Protein samples were prepared from 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Immunology 2022Quote: ... and Rec-G-Protein-HRP (Thermo Fisher Scientific, 101223, 1:2000). Blots were visualized with Enhanced Chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2021Quote: ... 5% glycerol) with 1% Halt protein/phosphatase inhibitor (ThermoFisher Scientific; 78446). Samples were spun at 17,100xg for 15 minutes at 4°C ...
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bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
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bioRxiv - Physiology 2021Quote: ... For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421), 1.5 mL of Y-3 antibody (0.5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... followed by trypsin (Thermo Fisher Scientific, 1:50 w/w protein) overnight at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-HuC/HuD neuronal protein (Invitrogen; 1/500; REF A21271), rabbit anti-laminin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
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bioRxiv - Biochemistry 2019Quote: ... supplemented with 1% Protein Free Hybridoma Medium II (PFHM II, GIBCO) (basal medium ...
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bioRxiv - Microbiology 2020Quote: ... the interacting proteins were crosslinked with 1% formaldehyde (28906, Thermo Scientific) for 10 minutes at room temperature and the reaction quenched by addition of 125 mM glycine (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... followed by immunoprecipitation for 1 h with Dynabeads Protein G (Invitrogen). The precipitated RNA was extracted with Trizol reagent and used for RT-qPCR analysis.
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bioRxiv - Neuroscience 2020Quote: ... lysosome-associated membrane protein 2 (LAMP2; 1:1000; Thermofisher, Waltham, MA), or phopsho-ribosomal S6 protein (PS6 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were identified using HRP-conjugated streptavidin (Invitrogen, 1:10000). After extensive washing in PBS containing 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 1% protein-free hybridoma medium (PFHMII, Thermo Fisher Scientific), 1% antibiotic-antimycotic (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... glial fibrillary acidic protein (GFAP) (Invitrogen, California, USA, 1:2000 dilution) and with HRP (horse radish peroxidase)-conjugated goat anti-rabbit IgG (secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were alkylated by 1 mM Pierce N-ethylmaleimide (ThermoFisher Scientific) in alkylation buffer containing 8M urea ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5-1 mL of Protein A beads (Thermo Fisher Scientific, 89898) were added and gently rocked overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Proteins were digested with 1 µg MS-grade trypsin (Thermo Fisher) at 37°C overnight with gentle shaking (800 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... anti-glial fibrillary acidic protein (GFAP; 1:1500, MA5-12023, Invitrogen), anti-CD68 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected using GLUT4 (1:2000, PA5-23052, Invitrogen, USA), AKT(1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Protein was digested with 1 μg trypsin (Thermo Scientific, Waltham, MA) at 37 ºC overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Genetics 2020Quote: ... gRNA/Cas9 complexes were formed by incubating 250 ng of the gRNA with 1 µg of purified Cas9 protein (TrueCut Cas9 Protein v2, Invitrogen) in OptiMEM (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... per 1 mg of total protein were added along with 30 μL washed protein A/G UltraLink Resin (53133, ThermoFisher) and rotated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Biochemistry 2020Quote: ... The pellet was re-suspended in 5mL of 1% SDC + 50mM ABC pH 8.5 and protein concentration was measured with Pierce BCA protein assay kit (Thermo Scientific). Samples were then aliquoted into 1.5mL LoBind eppendorf tubes (Sorenson BioScience ...
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bioRxiv - Bioengineering 2022Quote: ... using a ratio of 1 µg trypsin to 50 µg sample protein as quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Samples were digested overnight at room temperature using a rotor for mixing and then placed in a speed vacuum to reduce sample volume ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... which were pre-conjugated to 1:1 mix of 63 µL protein A/G dynabeads (Invitrogen), and incubated (4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked using 5% w/v non-fat dry milk in TBST for one hour at room temperature and then incubated with primary antibody (anti-Alix, 1:5000; Protein Tech, 12422-1-AP or anA-FloAllin-1, 1:5000; Fisher Scientific, BDB610820) at 4°C overnight ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in 1% NP-40 lysis buffer (58) and protein levels were quantified using the Bicinchoninic acid protein assay (Thermo Scientific). Samples were run on SDS-PAGE gels before being transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates from 30 wt eye imaginal discs (1 mg of total protein) was precleared by incubating with protein A-sepharose beads (Thermo Scientific) for 1h at 4 °C followed by centrifugation ...