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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 7α 12α Dihydroxycholest 4 en 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 5% FBS (certified One Shot, Gibco) supplemented with 100 ng/mL human GMCSF (Peprotech ...
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bioRxiv - Microbiology 2021Quote: ... One µL of propidium iodide (Gibco) was added to 15000 cells and incubated for 2-3 minutes ...
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bioRxiv - Microbiology 2021Quote: ... coli One Shot® TOP10 (Invitrogen). All strains and plasmids were confirmed by sanger sequencing.
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bioRxiv - Microbiology 2022Quote: ... coli One Shot BL21star (DE3) (Thermofisher) using the pGEX4T1 expression vector (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% Culture One (Thermo Fisher Scientific), 200 nM ascorbic acid (Carl Roth) ...
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bioRxiv - Molecular Biology 2022Quote: ... coli One Shot™ TOP10 (Invitrogen). The resulting plasmid was named pGID310-PagrB::gfp::bgaB.
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bioRxiv - Developmental Biology 2021Quote: ... One drop of DAPI (R37606, Invitrogen) was added to the cell suspension couple of minutes before the analysis with BD FACSAriaTM III sorter (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... and one drop of NucBlue (Invitrogen) along with the media and incubated at 37°C and 5% CO2 for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Nanodrop™ One (Thermo Fisher Scientific) was used to assess concentration of the purified pools ...
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bioRxiv - Immunology 2021Quote: ... one labeled with phycoerythrin (PE) (Invitrogen), and one with brilliant violent (BV ...
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bioRxiv - Immunology 2020Quote: ... one labeled with phycoerythrin (PE) (Invitrogen), one labeled with brilliant violet (BV ...
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bioRxiv - Biophysics 2019Quote: ... One ml of TRIzol reagent (ThermoFisher) was then added ...
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bioRxiv - Immunology 2021Quote: ... one mL of RPMI 1640 (Gibco) supplemented with 10% heat inactivated Fetal Calf Serum (FCS ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot™ TOP10 (Invitrogen). The recombinant gene insertion was confirmed by sequencing.
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot™ TOP10 (Invitrogen). The final construct was confirmed by sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... in one-part OptiMEM (ThermoFisher Scientific) and three-parts serum-starved cell-specific media (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... One milliliter of TRIzol reagent (Ambion) was added to the tube ...
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bioRxiv - Genomics 2023Quote: ... purity with Nanodrop One (Fisher Scientific), and MW and integrity with pulsed field electrophoresis (CHEF Mapper ...
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bioRxiv - Cancer Biology 2023Quote: ... quantified by Nanodrop One (Thermo Scientific), DNase digested and reverse-transcribed using the Improm-II Reverse Transcription System (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (One Shot Top10, Invitrogen C404003) were transformed with pCDH plasmids using the heat shock method (on ice for 30 min followed by 42 °C heat shock for 45 seconds) ...
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bioRxiv - Neuroscience 2023Quote: One step RT-PCR (Invitrogen #12594025) was used to isolate let-381 and unc-30 cDNAs (primers for let-381 ...
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bioRxiv - Developmental Biology 2023Quote: ... and a Nanodrop One (Thermo Scientific) was used to determine concentration ...
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bioRxiv - Biophysics 2019Quote: ... Rabbit anti GFAP antibody (kindly supplied by A. Triller, ENS Paris, 1:250) and Alexa 488 conjugated goat anti-rabbit (Thermo Fisher Scientific, Waltham, USA, 1:250), for neurons ...
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bioRxiv - Plant Biology 2023Quote: ... Chlorophyll content was then measured using a NanoDrop™One/One C Microvolume UV-Vis Spectrophotometer (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and (3) 110,000 × g for 2 hours at 4°C with a Sorvall Discovery SE ultracentrifuge (Thermo Fisher Scientific) with an AH-650 rotor (k factor ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Genomics 2020Quote: ... Two TaqMan probes targeting the MLLT3 exon 3-4 and exon 9-10 splice junctions (ThermoFisher, Hs00971092_m1 and Hs00971099_m1) were used with cDNA diluted 1:5 in dH2O to perform qPCR for six replicates per sample on an Applied Biosystems Quant Studio 6 Flex ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Cell Biology 2019Quote: ... Liposomes containing PtdIns(4)P- or PtdIns(3)P were independently labeled with DiI and DiD (Thermo Fisher Scientific) lipid dyes and detected by excitation at 561 nm and 640 nm ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Media was changed every 3 to 4 days and cells were passaged at nearly 90% confluence using 0.25% trypsin (Gibco). B16F0 cells were verified for mycoplasma contamination at Charles River Laboratories for cell line testing ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...