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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 20 μg of total protein (from whole cell extracts or isolated lysosomes) was separated with a 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and then transferred onto PVDF (polyvinylidene difluoride ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The fluorgenic substrate (5 μM) was mixed with control and test samples in 96 well plates (Black wall plate, Nunc), incubated for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were heat shocked in 50 μL (OD600 = 0.04) and transferred to plates (Nunc 96-Well Optical-Bottom Plate) containing 150 μL of their respective medium (final of OD600 = 0.01) ...
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bioRxiv - Microbiology 2020Quote: ... 10μl of lysate from the cell culture plate mixed then was transferred to a white Nunc 96-microwell plate (ThermoFisher) and 100μl of luciferase assay reagent (Promega Kit ...
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bioRxiv - Biochemistry 2022Quote: ... GTPase assays were performed in 200µl volumes in 96-well plates and the release of inorganic phosphate (Pi) was monitored at 360nm using the MultiSkan Go plate reader (ThermoFisher). Assays were performed in triplicates and a reference data set containing reaction buffer substrate (MESG ...
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bioRxiv - Immunology 2022Quote: ... the plates were read at multiple wavelengths (450 nm and 550 nm) on a plate reader (Multiskan GO, Thermo Fisher). The raw data were processed by Prism 9 (GraphPad ...
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bioRxiv - Bioengineering 2019Quote: ... the cells sampled at the same time points and diluted in PBS had their OD and YFP fluorescence (488nm excitation and 527nm emission) measured on a SynergyMX microtiter plate reader using clear-bottomed black microtiter plates (Thermofisher), and fluorescence values normalized to sample OD following the subtraction of background fluorescence from CEN.PK 113-7D ...
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bioRxiv - Synthetic Biology 2020Quote: Transformants were selected by plating first on YPD plates and then replica plating to yeast agar plates including 600 mg/L geneticin (Gibco) and incubating for a further 16 hours.
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bioRxiv - Biochemistry 2021Quote: 24.5 μL of 5 μM solution of every protein was prepared in 96-well plate MicroAmp Optical plate (Applied biosystems), and incubated at room temp ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... OPCs were seeded at 40,000 cells per well in 96-well plates or 50 million cells per 500 cm2 bioassay plate (166508, Thermo Fisher) (for generating large quantities of cells for sequencing studies ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL RNA + 95 μL H2O) into a new RNA dilution plate (96-well PCR quality plate – Thermo Scientific #AB0900). The final in-well concentration of the diluted RNA was in the range of 0.2 to 5 ng/μL.
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bioRxiv - Neuroscience 2022Quote: ... col seeded NGM plates and then moved to OP50 seeded plates containing 10 µM ThermoFisher scientific LysoTracker Blue DND-22 (Invitrogen) (1mM stock diluted 1:100 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 µL of culture with 26’000 cells was transferred in each well of the plate Nunc Edge 96-well plate (Thermo Scientific). The transfection mixture was added to the cells once it has incubated for approximately 30 min.
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bioRxiv - Cell Biology 2024Quote: HeLa cells were seeded at a density of 15,000 cell/cm2 in either 24-well plates or 12-well plates on #1.5 glass coverslips (Thermo Fisher Scientific). The following day ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fifty hydras were individually maintained at 18°C in cell culture plates (12-well plates, 1.5 mL/well, Thermo Scientific) filled with Volvic© water under a photoperiod of 12h ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Two microliters of each CFPS reaction were diluted with 33 μL of phosphate-buffered saline and analyzed in triplicate on a black 384-well plate with a multimode plate reader Varioskan Lux (ThermoFisher) at 488 nm excitation and 535 nm emission.
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Cell Biology 2023Quote: ... In a next step 25µl of the compound cell culture media mix were carefully transferred from the intermediate plate on plates with the cells using a Beckman Coulter Biomek Fx followed by incubation in the Cytomat incubator (ThermoFisher) at 37°C and 5% CO2 for 24 and or 72 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and the supernatants were transferred to a UHPLC-adapted 96-well plate (Nunc 96-well V-bottom polypropylene plate, ThermoFisher) and sealed ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 μM sgRNA stock plates were thawed and 5.76 μl of each guide was transferred to a 96 well conical bottom plate (Nunc, 249935) using a CyBio SELMA ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: Secreted IL-10 was determined by ELISA as per the manufacturer’s protocol (Mouse IL-10, Invitrogen, No: 88-7105-88). The color reaction was measured as OD450 units on a Bio-Rad iMark microplate reader ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: To measure the levels of secreted IL-17a from the Th17 cell culture assay we utilized the mouse IL-17a (homodimer) ELISA (ThermoFisher) according to the kit instructions.
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bioRxiv - Microbiology 2021Quote: ... Supernatants were assayed for IL-2 or IFNγ by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Thermo fisher).
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Immunology 2020Quote: ELISAs were performed using 50 ng of VP1 pentamer/well in an EIA/RIA Polystyrene High Bind Microplate (Fisher Scientific) coated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: Medium samples from cells and from the engrafted scaffolds were used to measure TGFβ using ELISA (88-8350-22, ThermoFisher), following manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Systems Biology 2024Quote: ... We detected proteins by chemiluminescence using the SuperSignal® ELISA femto maximum sensitivity substrate (Thermo Fisher Scientific™, Waltham, USA) according to the manufacturer’s instructions and the Amersham™ Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Cell Biology 2022Quote: ... HMGB1 was measured in cell-free supernantants using an enzyme-linked immunosorbent assay (Tecan HMGB1 ELISA, cat# ST51011, Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The plates were washed 3 times with PBS/Tween 20 0.05% (PBS-T) and saturated for 2 h at room temperature (RT) with 300 µl per well ELISA/ELISPOT diluent 1X (Invitrogen 00-4202-56 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the protein concentration was determined using the Pierce™ BCA Protein Assay Kit following the manufacturer’s instructions and a Varioskan Lux plate reader (Thermo Fisher Scientific, Waltham, MA, USA). Samples were separated using the Mini-PROTEAN® TGX™ Precast Gel 4-15% (Bio–Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Products from the second PCR were purified as described above and concentrations were normalized using SequalPrep Normalization Plate (96) Kit (Thermo Fisher Scientific, Waltham, MA, USA). We pooled and concentrated the libraries using DNA Clean and Concentrator-5 Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was conducted in 384 well plates using SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience, Cincinnati, OH, USA) in a QuantStudio 5 qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) in a QuantStudio 5 (384-well plate) qRT-PCR cycler (Applied Biosystems, Foster City, CA, USA). The qRT-PCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Microbiology 2019Quote: ... using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher), 0.2 μM of each primer and 1.5 μg of cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated PBMCs were cultured at a density of 5×105 cells/1 ml CM on 24-well plates coated with Geltrex (Thermo Fisher Scientific). After 24 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...