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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The crude exosubstance was diluted two-fold in PBS in a 96-well plate (ThermoFisher Scientific, Biolite Microwell Plates), leaving a final volume of 100μl in each well ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... a TECAN infinite f plex plate reader and black 96-well luciferase plates (Thermo Fisher Scientific Nunc A/S).
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... a TECAN infinite f plex plate reader and black 96-well luciferase plates (Thermo Fisher Scientific Nunc A/S).
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bioRxiv - Genetics 2021Quote: ... at a 1:1 (v:v) ratio and quantified using the Quant-iT PicoGreen dsDNA Assay kit (P11496, Invitrogen). 0.15 ng of cDNA in a 1.25 μl volume was used as input for Nextera XT (FC-131-1024 ...
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bioRxiv - Cell Biology 2021Quote: ... After two more XP beads purifications (1:1) libraries were quantified using Qubit dsDNA HS Assay Kit (Invitrogen). For Illumina flowcell production ...
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bioRxiv - Developmental Biology 2023Quote: ... After two more XP beads purifications (1:1) libraries were quantified using Qubit dsDNA HS Assay Kit (Invitrogen). For Illumina flowcell production ...
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bioRxiv - Genomics 2020Quote: We synthesized capped CLH-1 cRNAs using T7 mMESSAGE mMACHINE kit (Ambion) and purified by lithium chloride precipitation ...
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bioRxiv - Plant Biology 2019Quote: Machi3-1 protein was expressed with Pichia Expression Kit (Invitrogen K1710-01) using pPICz vector ...
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bioRxiv - Cancer Biology 2021Quote: ... LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit (1:1000, Invitrogen) was added to exclude dead cells ...
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bioRxiv - Pathology 2022Quote: ... Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems, 4391178) was used ...
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bioRxiv - Immunology 2022Quote: ... LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (1:1000, Invitrogen) was included in all stainings to exclude dead cells ...
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bioRxiv - Immunology 2020Quote: ... using a TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) or SYBR Green (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... using Power SYBR Green RNA-to-CT 1-Step Kit (ThermoFisher Scientific) on StepOne Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial RNA (1 ug) was reverse-transcribed using SuperScriptTM IV kit (Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... CD19 PeCy7 clone 6D5(1:200) and Bv510 (Live/Dead kit, ThermoFisher). Intracellular staining of GLUT1 Alexa Fluor 647 [EPR3915] (1:100 ...
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bioRxiv - Immunology 2023Quote: ... The TaqMan® RNA-to-CT™ 1-Step Kit (Applied Biosystems) was to perform reverse transcription and qPCR in the same reaction ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher Scientific, Cat# 4392938) was used in association with the following probes ...
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bioRxiv - Developmental Biology 2023Quote: ... and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific) using a LightCycler 480 instrument (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described97 ...
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bioRxiv - Developmental Biology 2021Quote: ... The coding sequence of EcI-4/Oatp58Db was PCR-amplified from whole-body Drosophila cDNA using Phusion Plus DNA Polymerase (Thermo Fisher Scientific) with the primers listed in Table S4 ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from 50 μL of whole lung homogenate or NHBE cell lysates and supernatant on a Kingfisher Flex Magnetic Particle Processor (Thermo Fisher Scientific) by using the Ambion MagMAX-96 AI/ND Viral RNA Isolation kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: The whole thalamus was collected from adult mouse brains (RosaCreErt−Shox2 KO) and immediately stored in RNAlaterTM RNA stabilization reagent (ThermoFisher Scientific, AM7021). RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... 10 µg of the whole cell lysate was separated using NuPAGE 4−12% Bis-Tris gradient gels (Invitrogen Life Sciences, Carlsbad, CA) and transferred to a PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described (23) ...
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bioRxiv - Microbiology 2019Quote: ... were flanked by self-cleaving ribozymes (a hammerhead ribozyme upstream and a hepatitis delta ribozyme downstream) and the whole sequence was ordered (GeneArt, ThermoFisher Scientific, UK) and cloned into a pSF-CAG-KAN vector (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... whole blood was collected using vacutainers and RNA was stabilized in the field using LeukoLOCK Total RNA Isolation System (Ambion life Technologies). The Poly(A)Purist Kit (Ambion Life Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... WBCs were isolated from human whole blood and also stained with a live cell marker (10 μM CMTPX, Invitrogen™, Carlsbad, USA). Detailed methods for MCF-7 cell culture and staining of cancer cells and WBCs are provided in our previous works 24-26 ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 100 µl of whole-blood specimens (diluted in 150 µl PBS) using TRIzol LS Reagent (Invitrogen/Life Technologies/Thermo) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 15 and 22 days after infection and genomic DNA was extracted from the whole abdomens of individual bees using DNAzol® reagent (Thermo Fisher). To quantify L ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole lung homogenates and bronchoalveolar lavage aliquots were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris gradient gel; Life Technologies, CA) and transferred to PVDF membrane ...
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bioRxiv - Pathology 2019Quote: Whole mount liver iDISCO+ cleared images were initially processed in ImageJ (NIH) and further filtered and analyzed in Amira (Thermo Fisher Scientific). Ink injected bile duct and portal vein filament tracing was performed on 2D images using Amira ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Total RNA was extracted from Drosophila S2 cells and whole female fly (one-week old) using TRIzol reagent and subjected to DNase treatment (Thermo Fisher #AM1907). The mRNA was then isolated through two rounds of poly-A selection using the oligo-d(T)25 beads (NEB #S1419S) ...
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bioRxiv - Developmental Biology 2019Quote: ... Total protein was extracted from the homogenized whole kidney lysates using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) supplemented with protease (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from whole blood and Complete exonic regions were amplified and sequenced on the Next generation sequencer (Ion Proton; Life technologies, USA). Functionally relevant variants were identified using Polyphen and SIFT scores ...
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bioRxiv - Cell Biology 2021Quote: ... Wells were coated with 100 μL of 20 μg/mL porcine fibronectin (prepared from whole blood) diluted in PBS at 37°C for 30 min and washed 3x using L-15 media (Gibco, 21083-027). Next ...
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bioRxiv - Evolutionary Biology 2020Quote: ... to ideally cut the whole organ into 5 µm sections across its entire length (microtomes: Leica 2065 or Microm HM 340E, Thermo Fisher Scientific). Sections were mounted on glass slides ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg purified carboxysomal proteins or 100 μg whole cell fractions were loaded per-well on 15% polyacrylamide gels and stained with Coomassie Brilliant Blue G-250 (ThermoFisher Scientific, UK).
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bioRxiv - Genomics 2022Quote: DGs were dissected by removing the whole sting complex of a dry-ice anesthetized bee and placing it in a drop of RNAlater (Invitrogen, Carlsbad, CA) to keep the tissue cold and prevent degradation ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg purified carboxysomal proteins or 100 μg whole cell fractions were loaded per-well on 15% polyacrylamide gels and stained with Coomassie Brilliant Blue G-250 (ThermoFisher Scientific, UK).
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bioRxiv - Cell Biology 2022Quote: ... DNA prepared from the whole-cell extracts or immunoprecipitated fractions was analyzed via quantitative PCR (qPCR) with the StepOnePlus Real-Time PCR system (Thermo Fisher Scientific) using Power SYBR Green PCR Master Mix© Green I Master (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: Whole-cell lysates were prepared in 1x Cell lysis buffer (CST, 9803) supplemented with protease and phosphatase inhibitor cocktail (Thermo Fisher, 78440), and total protein was measured by Pierce BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC) with a Dionex Ultimate 3000 UPLC system (Thermo Fisher Scientific) on a reversed-phase XBridge BEH C18 column (3.5 μm ...
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bioRxiv - Genomics 2023Quote: The LC-MS analysis for the full MitoIP metabolome profiling and 24-hour whole cell tracing samples was conducted on a QExactive benchtop orbitrap mass spectrometer coupled to a Vanquish UPLC System (Thermo Fisher Scientific). Prior to injection ...
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bioRxiv - Bioengineering 2023Quote: ... around 50 mg tumors and whole lymph nodes were ground with tube pestles in 100 μL ice-cold T-PER tissue protein extraction buffer (ThermoFisher, Cat # 78510) with HALT protease and phosphatase inhibitor (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... We made whole-cell lysates using RIPA lysis buffer and quantified amounts of protein per sample by BCA assay (23235, Thermo Fisher Scientific). We used primary antibodies against GIV (H-6 ...
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bioRxiv - Neuroscience 2023Quote: ... Whole brain masks and misaligned regions were segmented for the 3D dataset and DevCCF template in 3D using Avizo (Thermo Fisher Scientific). The segmented regions were subtracted from the brain masks ...
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bioRxiv - Plant Biology 2023Quote: ... One mL of each whole seawater sample was transferred to a clean 5 mL Falcon Round-Bottom Polystyrene test tube (ThermoFisher Scientific™). The volume of the sample was measured as the difference between the mass of the tube before and after flow cytometry processing divided by the density of seawater ...
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bioRxiv - Biochemistry 2023Quote: ... The remaining 95% of whole cell lysates were incubated using continuous overhead turning at room temperature with Pierce Streptavidin UltraLink Resin (Thermo Fisher Scientific) beads that were previously washed twice with lysis buffer ...