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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: 3T3-L1 fibroblasts (ATC-CL-173) were maintained in DMEM supplemented with 10% (v/v) FBS and penicillin-streptomycin (10000 U/ml, 1:100, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... MiaPaCa2 and KP4 cells [63,64,89] were obtained from ATCC and maintained in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS; Life Technologies, Carlsbad, CA) and 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... Media was changed to RPMI 1640 plus GlutaMAX (Cat. No. 61870-127, Life Technology) + 1% (v/v) Penicillin-Streptomycin (PS) (Cat. No. 15070-063, Thermo Fisher Scientific) + 1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: Undifferentiated ESCs and all differentiated cell types were crosslinked in culture by addition of methanol-free formaldehyde (ThermoFisher, final 1% v/v), and incubated at room temperature for 10 minutes with gentle rotation ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Microbiology 2023Quote: ... The A549 cell line (ATCC® CCL-185™) was maintained in DMEM medium supplemented with 10 % (v/v) fetal bovine serum (FBS) (Gibco) in a 5 % CO2 incubator at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in Complete Medium: DMEM (Cornell) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (GIBCO or Natocor), 1 mM L-glutamine (GIBCO) ...
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bioRxiv - Bioengineering 2023Quote: ... sodium bicarbonate supplemented with 10% fetal bovine serum and 1% (v/v) penicillin streptomycin (all reagents were purchased from Thermo Fisher Scientific). Cells were cultured in T25 cell culture flasks in a humidified air atmosphere with 5% CO2 at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat inactivated fetal bovine serum and 100 μg/mL penicillin-streptomycin (all Gibco™, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat inactivated fetal bovine serum and 100 μg/mL penicillin-streptomycin (all Gibco™, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... in 6-well cell culture plates (30006, SPL Life Sciences) coated with 1% (v/v) LDEV-free hESC-qualified Geltrex (A1413302, Thermo Fisher Scientific) in an incubator at 37 °C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: Tubular scaffolds were sterilized in a laminar flow cabinet by washing 3 times for 15 minutes in 5% penicillin/streptomycin (v/v, Gibco, Thermofisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Sporozoites were counted and diluted into infection DMEM (iDMEM) – cDMEM further supplemented with 1% (v/v) Penicillin-Streptomycin-Neomycin (Gibco 15640-055), 0.835µg/mL Amphotericin B (Gibco 15290-018) ...
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bioRxiv - Immunology 2022Quote: K562/HLA-E 45 and RMA-S/HLA-E 52 were maintained in complete medium (RPMI-1640 containing glutamine and supplemented with 10% v/v FBS, 20□μM β-mercaptoethanol and 100 U/mL penicillin-streptomycin; all Thermo Fisher) in the presence of 400□μg/mL hygromycin B and 1□mg/mL G418 (both InvivoGen) ...
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bioRxiv - Systems Biology 2022Quote: ... were used to perform buffer exchange (Phosphate-buffered saline [PBS, Gibco, Cat# 14190], 50 mM NaCl [Sigma-Aldrich, Cat# S5150-1L], 0.001 %, Pluronic F68 [v/v] [Gibco, Cat# 24040]) and the final concentration step ...
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bioRxiv - Microbiology 2022Quote: ... were provided by Martin Lowe (University of Manchester) and were cultured in DMEM supplemented with 10% (v/v) FBS (Gibco, 10500-064) and maintained in a 5% CO2 humidified incubator at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated in primary antibodies diluted in 5% (w/v) BSA in TBST with 0.01% (w/v) sodium azide (Fisher Scientific, S227I-25), rocking at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% (v/v) heat inactivated fetal bovine serum and 100 µg/ml penicillin-streptomycin (all Gibco™, Thermo Fisher Scientific). P19 WT cells were transfected with 4 µg plasmid DNA in 10-cm plates using the jetOPTIMUS® Transfection reagent (Polyplus ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% (v/v) heat inactivated fetal bovine serum and 100 µg/ml penicillin-streptomycin (all Gibco™, Thermo Fisher Scientific). P19 WT cells were transfected with 4 µg plasmid DNA in 10-cm plates using the jetOPTIMUS® Transfection reagent (Polyplus ...
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bioRxiv - Cell Biology 2024Quote: ... CVCL_0327), were maintained in DMEM (319-005-CL, Wisent) supplemented with 10% (v/v) fetal bovine serum (FBS, 12483020, Thermo Fisher Scientific) and 100 units/ml penicillin-streptomycin (450-201-EL ...
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bioRxiv - Genomics 2024Quote: Probes were hybridized to the sample at a concentration of 6-10 µM in a 30% v/v formamide (Fisher Scientific, AM9342), 10% w/v dextran sulfate (VWR ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed five times with PBS/Tween-20 (0.05% v/v; PBS/T) and blocked with 200 µL Blocker™ Casein in PBS (Thermo Fisher Scientific) for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... all cells were grown under standard conditions (37°C, 5% (v/v) CO2) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific, Waltham, MA) containing 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by DNA staining in 2X SSC/25% v/v formamide/1 ng/μL Hoechst 33342 (Thermo Fisher Scientific, cat. no. 62249) for 1 hour at 30°C ...
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bioRxiv - Plant Biology 2024Quote: ... pyridoxine hydrochloride + 0.01% (w/v) thiamine hydrochloride + 0.0002% (w/v) glycine + 175 nm N6-Benzyladenine + 10 μM EdU (Invitrogen A10044, Thermo Fisher Scientific) pH 5.7] containing 0.8% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... in RPMI 1640 containing 10 % (v/v) low endotoxin fetal bovine serum (FBS, BioTech, Prague, Czech Republic) and 1% penicillin-streptomycin (Gibco, Dublin, Ireland) and pretreated with 10 ng/ml of soluble anti-CD28 antibody (ExBio ...
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bioRxiv - Microbiology 2022Quote: ... (BEI NR-53726, RRID:CVCL_A7UJ) supplemented with 1% penicillin/streptomycin (MilliporeSigma, P4333) and 10% (v/v) fetal bovine serum (FBS) (Thermo Fisher Scientific, 26140-079). Calu-3 ...
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bioRxiv - Immunology 2023Quote: ... Blots were then wash 3 times in 15-30 mL TBST (0.05% v/v) prior to incubation in HRP-conjugated secondary antibodies (Invitrogen, REF:65-6120) in TBST (0.05% v/v ...
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bioRxiv - Genetics 2023Quote: ... DNA was purified from the lysates with an equal volume of 25:24:1 v/v phenol chloroform isoamyl alcohol (Thermo Fisher Scientific) in a 2 mL light phase lock gel tube (Quantabio) ...
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bioRxiv - Cell Biology 2023Quote: ... ATCC®) cells were grown in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (1:1, v/v) (#31331093, Thermo Fisher Scientific) supplemented with 20 mM HEPES (#15630–080 ...
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bioRxiv - Immunology 2024Quote: ... H2SO4 in distilled H2O (v/v) and OD values were immediately read at 450nm using a Multiskan GO plate reader (Thermo Fisher Scientific). Wash steps were conducted prior to addition of any reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2% (v/v) Gibco™ Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific, cat# A14132-02) in Nunc™ non-treated multidishes (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... of skimmed milk powder and then incubated for 2 h at RT or overnight at 4 °C in 3 ml of PBS 1X + 0.1% (v/v) Tween-20 in presence of a 1,000x dilution of anti GFP antibody (Rabbit anti-GFP, Invitrogen Thermo Fisher Scientific). After washing ...
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bioRxiv - Neuroscience 2024Quote: ... The DNA precipitate was washed three times in DNA-washing solution (70% ethanol [v/v] and 0.1 M NaCl) and transferred to 500 μl DNase-and RNase-free water (Gibco and Invitrogen, respectively). The DNA was quantified ...
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bioRxiv - Synthetic Biology 2024Quote: ... all ten reactions of double-digested pR-fp fragments were pooled into a 2mL-DNA LoBind Eppendorf tube and supplemented with 1/10 v/v of sodium acetate (3M, pH5.2) (Thermo Scientific, Cat# R1181), 0.05µg/µL of Glycogen (Thermo Scientific ...
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bioRxiv - Biophysics 2024Quote: ... RPTEC-TERT cells were cultured at 37°C with 5 % (v/v) CO2 and humidity in DMEM/F12 (Fisher Scientific, 11330-032) supplemented with RPTEC growth kit (ATCC ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Drosophila S2 cells were kept at 23-25°C in Schneider Drosophila medium with 10% (v/v) heat-inactivated fetal bovine serum and 1:100 penicillin–streptomycin (Gibco, Life Technologies). The pAC5.1-microRNA (200 ng ...
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bioRxiv - Molecular Biology 2024Quote: Digestions were acidified with trifluoroacetic acid (TFA, 0.5% v/v) and desalted on Pierce C18 spin columns (Thermo Fisher Cat No: 89870) with a wash of 0.5% TFA followed by elution in 70% acetonitrile 0.1% formic acid (FA) ...
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bioRxiv - Neuroscience 2024Quote: ... and the obtained peptides (dissolved in 0.5% (v/v) trifluoroacetic acid) were measured on a QExactive mass spectrometer coupled to an UltiMate 3000 RSLCnano HPLC system (Thermo Scientific, Germany). After absorption on a C18 PepMap100 precolumn (300 µm i.d ...
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bioRxiv - Cell Biology 2024Quote: ... Then CB were diluted 1:1 (v/v) into a dilution buffer (1mM EDTA in PBS supplemented by 2% FBS (Thermo Fisher, #10099141)) and undelayed with density gradient medium LymphoPrep (Stemcell Technologies ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration in epithelial cell lysates diluted 1:2 (v/v) in NaCl 0.9% was measured with Pierce Bradford Plus Protein Assay Kits (ThermoFisher Scientific, cat#23236). Calprotectin concentration was normalized to the protein level of each sample.
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bioRxiv - Neuroscience 2024Quote: ... the lobe containing the left MeA was cut away from the rest of the brain (which was drop-fixed in 4% v/v paraformaldehyde (Thermo Fisher, 28908) in 1x phosphate-buffered saline (PBS ...
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Intrinsically Disordered Regions Define Unique Protein Interaction Networks in CHD Family RemodelersbioRxiv - Biochemistry 2024Quote: ... the tryptic peptides were acidified to a final concentration of 2% (v/v) with formic acid and desalted using StageTips (Thermo Fisher Scientific). LC-MS/MS analysis was conducted using an UltiMate™ 3000 RSLCnano System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... We transferred supernatants to new 1.5 mL tubes and precipitated RNA using 10% (v/v) 3M sodium acetate (pH 5.5) (cat. no. AM9740; Invitrogen, Waltham, MA, USA), molecular biology grade glycogen to a final concentration of 1 µg/µl (cat ...
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bioRxiv - Biochemistry 2024Quote: ... 5% v/v acetonitrile [ACN] in water) before loading onto a PepMap C18 precolumn (300 µm × 5 mm, Dionex, Thermo Fisher Scientific) with 0.1% formic acid in 2 min ...
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bioRxiv - Bioengineering 2024Quote: ... Iodixanol was removed and buffer exchanged to phosphate buffered saline (PBS) containing 0.001% v/v Pluronic F68 (Gibco™, Grand Island, NY) using 7 kDa molecular weight cutoff Zeba™ desalting columns ...
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bioRxiv - Bioengineering 2024Quote: ... The pellet was washed with 600 µL 70% (v/v) ethanol and dissolved in 20 µL low TE buffer (Thermo Fisher Scientific) for 15 min at 65°C ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed four times in PBS supplemented with 0.05% (v/v) Tween-20 (PBS-T, IP wash buffer) using a DynaMag™ magnetic rack (Thermo Fisher, UK), transferred to a new tube and washed once in IP wash buffer ...