Labshake search
Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ~3 mL of serum from CV-A21-immunized or naïve mice was diluted in 97 mL of PBS and ran over a column pre-packed with CaptureSelect IgG-Fc (Multispecies) Affinity Matrix (ThermoFisher Scientific). IgGs were eluted with 0.1 M glycine buffer (pH 3.0 ...
-
bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... were cultured in F-12K Nut Mix medium supplemented with 5% (v/v) artificial FCS and with 0.5% (v/v) Insulin- Transferrin-Selenium (Thermo Fisher Scientific). BEAS-2B (Cat# CRL-9609TM ...
-
bioRxiv - Molecular Biology 2021Quote: ... HCT116 colorectal adenocarcinoma cells were maintained in McCoy’s medium supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO, Cat#16141079), 10 U/ml penicillin/streptomycin ...
-
bioRxiv - Biophysics 2020Quote: ... The amount of neutral red taken up was determined by measuring the absorbance at 540 nm using a Multiskan FC Microplate Photometer (Fisher Scientific). The CC50 values were calculated from best-fit dose response curves with variable slope in Prism 8.
-
bioRxiv - Microbiology 2020Quote: ... was used to detect circulating antibodies for LSDV in serum samples following the manufacturer’s protocol and analysed with the Multiskan FC Microplate photometer (Thermo Scientific™). Infectious virus titrations of PBMC suspension ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Hamster lungs were homogenized and diluted in Hank’s solution with the addition of 2% FCS and antibiotics (streptomycin sulfate and benzylpenicillin sodium salt, 200 U/ml) (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (59 ...
-
bioRxiv - Microbiology 2022Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (59 ...
-
bioRxiv - Immunology 2020Quote: ... Activated CD8+ T cells were maintained in DMEM medium (10% FCS) with 25 mM (HG) or 5.6 mM (NG) glucose (Thermo Fisher Scientific). Raji cells were maintained in RPMI 1640 medium (10% FCS ...
-
bioRxiv - Microbiology 2021Quote: ... Growth of each microbe was assessed by measurement of optical density (OD) readings at 600nm using a Multiskan FC Microplate Photometer (Thermo Scientific). Bacterial density in each well in the parent plate was then normalized individually to an OD600 of 1.0 using sterile filtered M9 buffer ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Inserts were gently washed and placed in serum free DMEM and the upper chamber filled with DMEM supplemented with 10 % FCS (Life Technologies) and either PBS or 100ng/ml FGF10 (Peprotech) ...
-
bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
-
bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
-
bioRxiv - Molecular Biology 2019Quote: ... female) cells and C2C12 (ATCC® CRL-1772™) cells were maintained in DMEM supplemented with 10% FCS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The amount of neutral red taken up was determined by measuring the absorbance at 540 nm using a Multiskan FC Microplate Photometer (Fisher Scientific). The CC50 values were calculated from best-fit dose response curves with variable slope in Prism 8.
-
bioRxiv - Immunology 2021Quote: ... Cells were resuspended in RPMI 1640 medium containing 10% FCS was labelled with 10μM CFSE dye (Thermo Fisher Scientific, catalogue# C34570) for 4 minutes at RT ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, GlutaMAX™, Pyruvate) supplemented with antibiotics and 10% FCS (Gibco, Life technologies) and maintained in T75 Flasks at 1×106 cells per flask ...
-
bioRxiv - Immunology 2020Quote: ... Absorbance was measured at 450 nm with a reference wavelength of 620 nm using the Multiskan FC Microplate Photometer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
-
bioRxiv - Physiology 2024Quote: ... The optical density of the reaction product was read at 450 nm using the Multiskan FC microplate reader (Thermo Scientific, USA) after the initial incubation period (3 min ...
-
bioRxiv - Neuroscience 2024Quote: ... substituted with 10% fetal calf serum (FCS; Capricorn Scientific, Palo Alto, CA, USA) and 2 mM L-glutamine (Invitrogen, Carlsbad, USA), 50 U/ml penicillin/streptomycin (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... The level of CV was quantified by measuring the absorbance at 540 nm with a MultiskanTM FC Microplate Photometer (Thermo Scientific). The average value from the blank (acetic acid only ...
-
bioRxiv - Immunology 2024Quote: ... at a concentration of 250µg/ml in culture media (RPMI 1640 supplemented with 10 % FCS, 55 µM β-mercaptoethanol and Penicillin-Streptomycin-Glutamin 100x (Gibco)) for 2h at 37°C and washed twice ...
-
bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; PAN-Biotech, Aidenbach, Germany, #P30-3306) and penicillin–streptomycin (100 U/ml; Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... The reactions were stopped by adding 100 µL H2SO4 (0.5 M) to each well and absorbances were measured in a microplate reader (Multiskan FC; Thermo Fisher Scientific) at 450 nm and 620 nm.
-
bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
-
bioRxiv - Bioengineering 2023Quote: ... TF-1 were purchased from DSMZ (Cat#ACC334) and maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies), 2mM GlutaMAX and 2ng/ml hGM-CSF (215-GM ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). The MA104/NSP5-BioID2 cell line was generated using a lentiviral system ...
-
bioRxiv - Immunology 2023Quote: ... were performed according to the manufacturer’s instructions with 50 000 cells per well and read at 492nm on a microplate absorbance reader (Multiskan FC microplate photometer, Thermo Fisher).
-
bioRxiv - Immunology 2023Quote: ... in PBS for 10 min prior to addition of the relevant surface marker fluorochrome-conjugated antibodies in FACS buffer (PBS, 2% heat-inactivated FCS) supplemented with Super Bright staining buffer (Thermo Fisher). Following 30 min of incubation ...
-
bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
-
bioRxiv - Physiology 2023Quote: ... Purified Fc-FGF-21CT was subject to an endotoxin removal proces (Pierce High Capacity Endotoxin Removal Spin Columns, Thermo Fisher Scientific) before flash-frozen and stored at -80°C until use ...
-
bioRxiv - Cancer Biology 2023Quote: ... 7AAD-CD45-CD235A-CD71- non-hematopoietic fraction was sorted in DMEM supplemented with 10% FCS for Single-cell RNA sequencing and 7AAD-CD45-CD235A-CD71-CD31-CD274+ BMSCs were sorted in TRIzol (Life Technologies) for bulk RNA sequencing.
-
bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
-
bioRxiv - Neuroscience 2024Quote: ... DRGs or TGs were isolated and digested with 1 mg/ml collagenase and 2.5 mg/ml dispase dissolved in ‘basal medium’ (Neurobasal A medium supplemented with 10% FCS) (all from Gibco/Thermo Fisher) at 37°C for ca ...
-
bioRxiv - Neuroscience 2023Quote: ... were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, PAA Laboratories GmbH, Colbe, Germany) containing 10% fetal calf serum (FCS, Invitrogen, Carlsbad, California, USA), and 2 mM L-glutamine (PAA laboratories GmbH ...
-
bioRxiv - Immunology 2024Quote: ... we cultivated the corresponding rat hybridomas in IMDM (Gibco, 12440-053; supplemented with 10 % FCS, 1X MEM Non-essential amino acid solution (ThermoFisher, 11140050), 2 mM Glutamax (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Rspo3-Fc Fusion Protein Conditioned Medium (Immunoprecise, #R001-100ml, 2%)] and mixed with 50 µl Matrigel (Fisher Scientific, #CB-40230C). Human alveolar organoid media was refreshed every 3 days until the end of the experiment ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). MA/cytBirA (24 ...
-
bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
-
bioRxiv - Cell Biology 2019Quote: ... 24 aliquots of human blood (20 μL) from each donor were pipetted into 1 mL 2D-barcoded tubes (Matrix Storage Tubes; Thermo Fisher Scientific Inc., Waltham, MA), placed into a ANSI/SLAS microplate format compatible 96-tube racks (8 × 12 ...
-
bioRxiv - Immunology 2020Quote: ... Immune mediator levels in COVID-19 patient plasma samples across acute samples were measured with by Luminex using the Cytokine/Chemokine/Growth Factor 45-plex Human ProcartaPlex™ Panel 1 (ThermoFisher Scientific, #EPX450-12171-901). Data acquisition was performed on FLEXMAP® 3D (Luminex ...
-
bioRxiv - Pathology 2023Quote: Immunohistochemical staining for human claudin-2 was performed on formalin fixed paraffin embedded human pancreas tissue sections by using a mouse-anti claudin-2 (1:100, Invitrogen Cat# 32-5600, RRID: AB_2533085). Pancreas sections were deparaffinized ...
-
bioRxiv - Immunology 2022Quote: ... from normal donors were obtained with an NIH-approved protocol and activated with CD3 and CD28 microbeads at a ratio of 1:3 (Dynabeads Human T-Expander CD3/CD28, Thermo Fisher Scientific, catalog no. 11141D) in AIM-V media containing 40 IU/mL recombinant IL-2 and 5% FBS for 24 hr ...
-
bioRxiv - Microbiology 2023Quote: Single-cell suspensions of the human iPSC-derived lung organoids were treated with 1 × Permeabilization Buffer (e-Bioscience, Thermo Fisher Scientific, Cat# 00-8333-56), and then incubated with APC-labeled anti-human HLA class 1 (R&D systems ...
-
bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
-
bioRxiv - Cell Biology 2023Quote: NHK/38 cells and sections of normal human epidermis were stained with a monoclonal anti-hIL-38 antibody (H127C) (14-7385-82, Thermo Fisher Scientific AG, 1/2000), followed by an Alexa Fluor 594-labeled goat anti-mouse IgG2b (115-585-207 ...
-
bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...