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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and activated with Dynabeads Human T Activator CD3/CD28 (Gibco, USA) at a 1:1 ratio for 24 hours ...
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bioRxiv - Immunology 2023Quote: Adipocytes were differentiated from human adipose-derived stem cells (ADSCs, ThermoFisher), which were obtained from human lipoaspirate tissue and cryopreserved from primary culture ...
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bioRxiv - Immunology 2023Quote: ... were obtained from ThermoFisher and maintained in Human Large Vessel Endothelial Cell Basal Medium (ThermoFisher) according to manufacturer’s protocol.
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... Cytokine & Chemokine Convenience 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) was used to collect and analyze the supernatant for levels of cytokines/chemokines following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... CCL5 and CCL2 (Human cytokine magnetic 25-plex panel; Life technologies).
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bioRxiv - Immunology 2023Quote: ... and erythrocytes using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, 11350D) (Supplemental Figure 2).
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bioRxiv - Developmental Biology 2023Quote: ... KiPS line was derived by reprogramming of commercial human keratinocytes (Invitrogen) with Sendai viruses encoding for OSKM 62 and kindly provided by Austin Smith’s laboratory ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney HEK293T cells were maintained in DMEM/F12 (Gibco) with 10 % FCS (Gibco ...
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bioRxiv - Genomics 2023Quote: Commercially available human iPSC lines derived from a female donor (Gibco Episomal hiPSC ...
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bioRxiv - Cancer Biology 2024Quote: ... added with human complete organoid medium containing Advanced DMEM/F12 (GIBCO), 10 mM HEPES (GIBCO) ...
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bioRxiv - Molecular Biology 2024Quote: Human MET knockout HeLa cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols in a 6-well plate format ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/ml human basic FGF recombinant protein (bFGF) (Gibco, 13256029), 2% B27 supplement (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20 ng/ml human EGF recombinant protein (Gibco, PHG0314), 20 ng/ml human basic FGF recombinant protein (bFGF ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... miBrains were cultured in Human Endothelial Serum-Free Medium (ThermoFisher Scientific) with 1% pen/strep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: The following human (h) and rat (r) TaqMan assays (Thermo Fisher) were used ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher). For the appraisal of common painkillers drugs were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... and 2.5 ng/mL recombinant human hepatocyte growth factor (Gibco, UK).
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels were measured using Human Uncoated ELISA Kits (ThermoFisher) targeting IFNψ ...
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bioRxiv - Developmental Biology 2024Quote: ... Complete human medium with 10% exosome depleted FBS (Gibco, Massachusetts, US) was replenished and device placed into incubator ...
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bioRxiv - Cell Biology 2024Quote: ... Human Pools Set v3.0 kit (Applied BiosystemsTM, Thermo Fisher Scientific, USA) and the TaqManTM MicroRNA Reverse Transcription Kit (Applied BiosystemsTM ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... Normal human epithelial cells were cultured in keratinocyte-SFM medium (Gibco) supplemented with 1% antibiotic-antimycotic (Gibco).
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bioRxiv - Genomics 2024Quote: We used the human Amyloid-β 40 (ThermoFisher Cat No. KHB3481), Amyloid-β 42 (ThermoFisher Cat No ...
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bioRxiv - Immunology 2024Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Biophysics 2020Quote: ... The confocal parameters were calibrated daily by measuring the FCS decay of a 20 nM TAMRA (carboxylic acid of tetramethyl rhodamine) free dye solution (Molecular Probes, Inc.) with a known diffusion coefficient (D = 420 μm2s−1 ...
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bioRxiv - Cell Biology 2020Quote: ... FCS (10% FCS-DMEM/F-12) at 37°C under 5% CO2/95% air in a Heracell 150i CO2 incubator (Thermo Fisher Scientific). Cells were passaged every 2 days or once they had reached 80% confluency ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were incubated with 5 μg/mL anti-mouse CD16/CD32 Fc block (clone 2.4G2, Thermo Fisher Scientific, Cat. #553142) for 15 minutes at 4°C per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Optical density of standards and samples were measured at OD595 nm using a Multiskan™ FC Microplate Absorbance Reader (Thermo Scientific, Belgium). Plasma and cell lysate were prepared by mixing 100 µl citrated plasma with 100 µl RIPA buffer and 6 × 106 HUVECs with 250 µl RIPA ...
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bioRxiv - Microbiology 2022Quote: Expression plasmids encoding CLII or CLIV with an Fc- and His6-tag were transfected in Expi293F expression cells (Gibco-Thermo Fischer Scientific) using the ExpiFectamine293 transfection kit ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation (500 x g, 5 min) and washed with CO2-independent medium supplemented with 10 % FCS (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Bioengineering 2020Quote: ... NKG2DL expression was assessed by staining with NKG2D-Fc chimera (10 µg / mL; Fisher 1299NK050) followed by an αFc secondary stain (Invitrogen #A-10631). NIR Live/Dead (ThermoFisher #L34976) ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Cell Biology 2021Quote: ... and A9 cells (obtained from Nobuo Takagi at Hokkaido University in 1980s) were cultured in DMEM (Nacalai Tesque) with 10% FCS (Thermo Fisher Scientific) and 1% GPS solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with heat-inactivated 10% v/v foetal calf serum (FCS; HyClone) and 500 μg/mL Geneticin (Gibco cat no 10131-0275) and kept under 5% CO2 at 37°C.
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bioRxiv - Bioengineering 2022Quote: ... The absorbance at a wavelength of 450 nm was measured using Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA USA).
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Proteins were quantified with Image Studio Version 5.2.4 (LI-COR Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: ... Pro + using an optimized autoinduction media and purified by protein A affinity chromatography similarly to Fabs.30 VH-Fc were expressed in Expi293 BirA cells using transient transfection (Expifectamine, Thermo Fisher Scientific). 4 days after transfection ...
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bioRxiv - Microbiology 2020Quote: ... spun at 200 × g for 10 min and resuspended in macrophage medium (DMEM [Invitrogen] plus 10% FCS and 2 mM L-glutamine) in order to have 2.5 × 106 cells per well ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded at a density of 2.7×104 cells/cm2 and cultured in William’s Medium E (Biochrom, Berlin, Germany) containing 10% (v/v) FCS (Life Technologies, Darmstadt, Germany), 5 mg/ml insulin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, GlutaMAX™, Pyruvate) supplemented with antibiotics and 10% FCS (Gibco, Life technologies) and maintained in T75 Flasks at 1×106 cells per flask ...
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bioRxiv - Cell Biology 2020Quote: ... NIH3T3 and RPE-1 were maintained in DMEM (HPSTA – high glucose, stable glutamine and sodium pyruvate; Capricorn Scientific) supplemented with 10% fetal calf serum (FCS; Thermo Fisher Scientific) under standard conditions at 37°C in a 5% CO2 environment ...
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bioRxiv - Microbiology 2021Quote: ... OD595 was measured every 5 min at 37°C from cultures that underwent continuous shaking in a Multiskan™ FC Microplate Photometer (Thermo Scientific). Data were collected from at least two independent experiments with at least two replicates for each individual experimental condition.
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bioRxiv - Microbiology 2021Quote: ... Two photometric readings were conducted for each plate with a 540 nm filter on a Multiskan FC Microplate Photometer (Thermo Scientific 51119000). The protocol is a slightly modified version of an Enzymatic Assay of α-Amylase (https://www.sigmaaldrich.com/NL/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase ...
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bioRxiv - Immunology 2022Quote: ... 100 µl of 0.2 mM sulfuric acid was added to halt the enzymatic reactions and the optical densities (OD) of the contents in the wells were read at 450 nm using a Multiskan FC plate reader (Thermo Scientific, USA).
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bioRxiv - Cell Biology 2022Quote: ... the absorbance of each well at 450 nm was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (BioConcept, Cat# 2-01F16-I) and 100 units/ml of penicillin and 100 µg/ml of streptomycin (Invitrogen, Cat# 15140122). Rat primary fibroblasts (REFs ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...