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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Candida albicans enhance Staphylococcus aureus virulence by progressive generation of new phenotypesbioRxiv - Microbiology 2024Quote: Quantitative real-time PCR (qRT-PCR) was performed with the PowerUp SYBR Green Master Mix (Applied Biosystems) in a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... using the SuperScript III RT-PCR kit (Life Technologies), employing reverse transcriptase in the presence of deoxynucleotides (dNTP ...
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bioRxiv - Molecular Biology 2022Quote: ... Using Verso 1-Step RT-PCR kit (Thermo Scientific), reactions of 13 μl final volume was set up using 0.6 μM of triplex primer cocktail in equimolar ratio of labelled forward and reverse primers of each target ...
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bioRxiv - Immunology 2023Quote: ... Quantitative real-time PCR was performed with Thunderbird SYBR qPCR Mix (#QPS-201, TOYOBO) on the StepOne Real-Time PCR System (Applied Biosystems, Kanagawa, Japan). The nucleotide sequences (from 5’ to 3’ ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The determination of the absolute number of viral copies was made by a Taqman real-time qPCR assay with the ad of the StepOneTM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). A standard curve was generated in order to obtain the exact number of copies in the tested sample ...
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bioRxiv - Pathology 2020Quote: Quantification of individual mRNA expression was performed by real-time PCR on an ABI 7500 Fast Real Time System (Applied Biosystems, Foster City, CA, USA), using TaqMan probes as previously described [18].
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Real-time PCR was done using a BioRad CFX384™ real-time machine with SYBR master mix (Life Technologies/Applied Biosystems, NY, USA Cat#4367659). Transcript levels were assayed triplicates and normalized to beta-actin ...
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bioRxiv - Cell Biology 2020Quote: ... Cycle threshold was calculated by using default settings for the real-time sequence detection software (Thermo Fisher). For relative expression analysis the quantity of each sample was first determined using a standard curve and normalized to GAPDH and the average target gene expression (deltaCt/average target gene expression).
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bioRxiv - Genomics 2021Quote: ... Real-time amplifications were performed on the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems, Tokyo, Japan). The settings for the thermal profile were an initial denaturation (30 s at 95°C ...
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bioRxiv - Genetics 2020Quote: ... Amplification reactions were run on a Quanstudio5 384-system real time detection system (Thermo Fisher Scientific, USA). Reactions consisted of a Pre-Read stage (60°C for 30s) ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative PCR (qPCR) was performed with SYBR Green PCR Master Mix Kit on a QuantStudio 3 system (Applied Biosystems, Foster City, CA). GAPDH was used as a housekeeper gene for the normalization of gene expression ...
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bioRxiv - Microbiology 2021Quote: ... The quantitative real-time PCR assay was performed with SuperScript III Platinum SYBR Green One-Step qRT-PCR kit (Thermo Scientific, Wilmington, DE, USA). For each sample ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription and PCR amplification were performed using TaqMan RNA-to-CT 1-Step Kit and the StepOnePlus™ Real-Time PCR Instrument (Applied Biosystems, Fisher Scientific). All primers used in this study are listed in Table S1.
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bioRxiv - Neuroscience 2021Quote: ... Real-time RT-qPCR was carried out with the PowerUp SYBR Green Mastermix (ThermoFisher Scientific, Vilnius, Lithuania) at the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with a SYBR Green PCR Master Mix and the 7900 Real time PCR system (Applied Biosystems). Results were analyzed by the standard curve method and normalized to β-actin expression ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was used as template for quantitative PCR with PCR amplification detected with Sybr green (SYBR Green Real-time PCR master mixes, Invitrogen). See the table below for the sequences of primers used to quantify real time amplification.
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, Slovakia) and a QuantStudio 5 thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The quantitative PCR (Q-PCR) reactions were conducted in triplicate on a ViiA 7 Real-Time PCR system (Thermo Fisher) from 1 μL of cDNA using the PerfeCTa SYBR Green SuperMix reagent (Quantabio Cat# ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed in a Quantstudio 5 Real-Time PCR Instrument using SYBR Green PCR Master Mix (Applied Biosystems). Three biological replicates were carried out ...
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Mitochondrial ROS1 increases mitochondrial fission and respiration in oral squamous cancer carcinomabioRxiv - Cancer Biology 2020Quote: ... Q-PCR was performed using Power SYBR Green PCR Master Mix and ABI StepOnePlus Real-Time PCR System (Applied Biosystems) with specific primers ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed using SYBR Select PCR master mix on a ViiA7 Real-Time PCR System (both from ThermoFisher). Fold changes were calculated by the 2−(ΔΔCt ...
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bioRxiv - Biochemistry 2022Quote: The sensor chips were 12-542-B (Thermo Fisher) glass coverslips coated with 3 nm of chromium and 47 nm of Au ...
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bioRxiv - Molecular Biology 2019Quote: ... RT-PCRs were performed using the One-step Superscript III RT-PCR kit with Platinum Taq polymerase (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... with a one-step RT-PCR kit using Taqman® technology (AgPath-IDTM One-Step RT-PCR, Life Technologies) and using the Applied Biosystems ViiA 7 Real-Time PCR System and the appropriate primers for Taqman assays (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... A multi segment RT-PCR amplification was performed using the Superscript III high-fidelity RT-PCR Kit (Invitrogen, USA). Influenza virus specific primers were used ...
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bioRxiv - Immunology 2024Quote: ... and viral GP gene amplified by RT-PCR (Verso 1-Step RT-PCR kit; Thermo Fisher Scientific AB-1455), cloned and sequenced as previously described 48.
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bioRxiv - Genomics 2024Quote: ... We then compared the performance of the different kits in terms of detection and quantification rates using standard RT-PCR (Applied Biosystems 7500 Fast Instrument, Thermo Fisher) and portable RT-PCR (Magnetic Induction Cycler quantitative PCR (Mic qPCR) ...
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bioRxiv - Immunology 2023Quote: ... The number of SIV copies was normalized to diploid genome cell equivalents co-determined by Q-RT-PCR using the TaqMan™ RNase P Detection Reagents Kit (Applied Biosystems), as described previously (11 ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and the synthesized cDNA was used for real-time analysis using the SYBR Green PCR Master Mix kit (Applied Biosystems) in the the ABI 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... reactions were performed using 2 μl of diluted cDNA as the template and gene-specific primers in triplicate by Power SYBR Green Kit and in a StepOne Plus Real-Time PCR System according to manufacturer’s instructions (Applied Biosystems) as described earlier (Murmu et al. ...
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bioRxiv - Microbiology 2019Quote: ... which were carried out with Kapa Sybr Fast Universal Kit (Biosystems) in a 7500 Real Time PCR System (Applied Biosystems). The T ...
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bioRxiv - Microbiology 2021Quote: ... and the synthesized cDNA was used for real-time analysis using the SYBR green PCR master mix kit (Applied Biosystems) in the ABI 7500 Fast real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Real time-PCR amplification of the synthesized cDNA was conducted using the Fast SYBR Green Master Mix kit (Life Technologies). Each of the three biological replicates was analyzed in technical triplicate on the StepOnePlus platform (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). PCR amplicons were quantified against in vitro transcribed RNA N gene fragment standard ...
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bioRxiv - Neuroscience 2023Quote: ... was performed in a Bio-Rad CFX384 real-time PCR machine using 2X DyNAmo HotStart SYBR Green qPCR Kit reagents and methods (ThermoFisher) and gene transcript-specific primer pairs (Extended Table 12-1) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time PCR with three multiplex wells once with TaqMan gene assay kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Quantitative real-time PCR was performed using the TaqMan® RNA-to-Ct™ 1-Step Kit (Thermo Fisher, #4392653) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Maxima First Strand cDNA Synthesis Kit was used to generate the cDNA template for quantitative real-time PCR (Thermo Fisher). RT-PCRs were carried out with Applied Biosystems StepOne Plus and analysed using the corresponding software StepOne Software Version 2.0 (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... and the synthesized cDNA was used for real-time analysis using the SYBR green PCR master mix kit (Applied Biosystems) in the ABI 7500 Fast real-time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... PCR was performed using one step SuperScriptR RT-PCR kit (Invitrogen, Carlsbad, CA) with E7 specific primers ...
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bioRxiv - Immunology 2023Quote: ... Bacterial burden of MAC at the time points previously listed was determined by quantitative real-time PCR (qPCR) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA) and primers/probe specific for the IS1311 insertion sequence found in M ...
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bioRxiv - Cancer Biology 2021Quote: ... Amplification was performed with a 7500 Fast Real-Time PCR Systems (Applied Biosystems, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Quantitative real-time PCR was performed with power SYBR green master mix (Applied Biosystems) using a 7300 real-time polymerase chain reaction machine (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Semi-quantitative real-time PCR was carried out (Thermo Fisher Scientific, 18080085 and 4385617) as previously described (Endo et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... ViiA 7 Real-Time PCR System was used to perform the reaction (Applied Biosystems). Values were corrected by b-actin expression ...
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bioRxiv - Cancer Biology 2021Quote: ... and run on an Applied Biosystems QuantStudio 3 real-time PCR machine (Thermo Fisher). Relative transcript levels were calculated using the ΔΔCT method and normalized to the ACTB gene ...
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bioRxiv - Cancer Biology 2021Quote: ... ViiA 7 Real-Time PCR System was used to perform the reaction (Applied Biosystems). The average and standard deviations were assessed for significance using a student’s t-test ...
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bioRxiv - Systems Biology 2020Quote: ... The Taqman method was used for real-time PCR with primers from Life technologies. The expression of mRNA was normalized to the geometrical mean of 3 house-keeping genes ...