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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (Invitrogen catalogue number R780-07). Cell cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Genetics 2019Quote: HEK293 cells (CRL-1573, ATCC, VA, USA) were cultured in high-glucose DMEM (Cat. No. 11965092, Life Technologies, USA) supplemented with 10% FBS at 37 and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
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bioRxiv - Biophysics 2021Quote: ... The HEK293 cells were transfected with 100 ng/well of pGL3-Fluc plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol at around 75-90% confluency in a 96 well plate ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides from HEK293 and HeLa cells were respectively injected into LTQ-Orbitrap Velos and Q Exactive (Thermo Fisher) onto a 75 μm i.d ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney (HEK293) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L Glucose and GlutaMAX™ (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with GFP constructs (250 ng/well) for 24 (HEK293) or 48 (SH-SY5Y) hours using Lipofectamine 3000 (0.75 µL/well; Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells, HEK293 or MEF cells were cultured in 10% FBS (Hyclone, SH3007103) in high glucose DMEM (Gibco 11965118) with 1% penicillin/streptomycin at 37°C ...
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bioRxiv - Immunology 2021Quote: HEK293 freestyle cells were transfected with this expression construct in combination with pBu-BirA using 293Fectin (Thermo fisher scientific) transfection reagent at 1μg DNA/10^6 cells ...
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bioRxiv - Neuroscience 2022Quote: ... which were used to directly transfect HEK293-Kir2.1 cells with the linear product using lipofectamine 3000 (Thermo Fisher Scientific). For patch clamp characterization in HEK293A cells ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293E or HEK293-6E cells were maintained in suspension cultures in Freestyle 293 Expression Media (Gibco, Waltham, MA, USA) supplemented with heat-inactivated fetal calf serum (1% ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Cells (primary neurons or HEK293 cells) were transfected with 1.6 µg/mL of a pIRES2-EGFP plasmid vector (Invitrogen) harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number ...
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bioRxiv - Immunology 2019Quote: ... we performed immunoprecipitation of CT-DNA stimulated HEK293 cells using the antibody to IRF3 pS386 crosslinked to Dynabeads (ThermoFisher) overnight at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Neuroscience 2020Quote: ... were subcloned into the polylinker of the pcDNA3.1 vector (Invitrogene, USA) by standard techniques for transiently transfecting HEK293-F cells (ThermoFisher). Cells were transiently transfected with the plasmids containing the desired GABAAR subunit cDNAs (α ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cell lines were transfected with pOG44 and pcDNA5/FRT/TO plasmids harboring respective FLAG constructs using Lipofectamine3000 (Invitrogen) as transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... Stable cell lines were made by transfecting HEK293 cells with the respective plasmids using Lipofectamine 2000 (Invitrogen, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... HEK293T TRIM21 knock-out and HEK293 HOIP KO cells were maintained at 37°C in complete DMEM (ThermoFisher 10569010) supplemented with 10% FBS and pen/strep.
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI- cells were grown to approximately 90 % confluency on 24-well tissue culture plates (cat# 11391704, Fisher Scientific) before the medium was exchanged for serum reduced DMEM and the transfection mixture was added ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were generated by integrase-mediated homologous recombination of the CFTR sequence into the T-REx HEK293 background (ThermoFisher Scientific). This expression system allows the tetracycline-inducible expression of genes of interest from one defined insertion site ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Parental T-REx-HEK293 cells were cultivated in growth medium: Dulbecco’s modified Eagle’s medium (DMEM) + GlutaMAX-I (#31966, Life Technologies), 10% Dialyzed FBS (Gibco ...
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bioRxiv - Bioengineering 2022Quote: Flasks of HEK293 cells were pre-labelled red or green using cell tracker labels (C7025 and C34552, Thermofisher Scientific) or blue using Hoescht 33342 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were maintained in HEK293 culture medium – Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate; Gibco, #41966029) with 10% foetal calf serum (FCS ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... Hek293-T cell lines were cultured in DMEM (ATCC; 30-2002) supplemented with 10% FBS (Thermo Fisher Scientific; 10082147), 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Cell Biology 2023Quote: ... wild type HEK293 or HEK293A (for figure 1A) cells were transfected with the indicated plasmids with Lipofectamine2000 Reagent (Invitrogen) as per manufacturer protocol ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney (HEK293) and A549 cell lines were maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Lithuania) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: NIH3T3 derived Bmal1-luc31 and HEK293/T17 cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Gibco: Cat #11965118) supplemented with 10 % fetal bovine serum (Corning ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco, #11965092) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 cells were incubated in 5% CO2 at 37 °C and passaged every 72 hr with trypsin (Gibco, #25300054) not allowing them to reach confluency.
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bioRxiv - Cell Biology 2024Quote: U251 MG cells (ATCC, US), HeLa cells (ECACC, UK), and HEK293 cells (ECACC, UK) were cultured in DMEM (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... which were used to directly transfect HEK293-Kir2.1 cells with the linear product using lipofectamine 3000 (Thermo Fisher Scientific). For characterization in HEK293A cells ...
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bioRxiv - Physiology 2024Quote: The MIC60 and CHCHD6 siRNAs along with scramble siRNA control were transfected into HEK293 cells using Lipofectamine RNAiMax (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies used were: mouse IgG2a anti-V5 tag monoclonal antibody (Thermo Fisher Scientific Cat# R960-25 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were labelled with 10-plex Tandem Mass Tag reagents (TMT10, Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... anti-V5 tag monoclonal antibody (Thermo Fisher Scientific, Cat #R960-25, 1:5000), anti-myc tag polyclonal antibody (MBL ...
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bioRxiv - Cell Biology 2022Quote: ... and peptides were labelled with Tandem Mass Tags (Cat#TMT; 90061, ThermoFisher Scientific).