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Citations for Thermo Fisher :
3751 - 3800 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and were maintained in DMEM high glucose (4500 mg/L) supplemented with 10% fetal bovine serum (FBS) (AlphaFBS-HI, Alphabioregen) and 1% Penicillin/Streptomycin antibiotics (Gibco), incubated at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
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bioRxiv - Genomics 2022Quote: ... The purified protein was analyzed by SDS Page electrophoresis and western blot analysis using anti-His antibodies as per manufacturer guidelines (ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... Sequencing templates were prepared on the Ion Chef System using the Ion PI Hi-Q Chef Kit (Thermo Fisher Scientific). Sequencing was performed on an Ion Proton System using an Ion PI v3 chip (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged proteins were detected on film by chemiluminescence using a horseradish peroxidase-conjugated His antibody and SuperSignal West Femto substrate (ThermoFisher).
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bioRxiv - Systems Biology 2023Quote: ... then eluted by incubation for 20 min in 11 µL Formamide-ROX350 mix (1000 µL Hi-Di Formamide (Thermo Fisher) and 8 µL ROX350 ladder (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... Expression vectors were made by cloning the indicated coding sequences into the pcDNA3.1/V5-His-TOPO vector (cat. no. K480001; Life Technologies). Constitutively active STAT5B (STAT5BCA ...
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bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Cell Biology 2024Quote: All eukaryotic expression plasmids for pUL71 and pUL71 homologues were generated in the backbone of pEF1/Myc-His C expression vector (Invitrogen) by cloning using restriction enzymes ...
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bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... C2C12 cells transfected with EGFP-HRas WT/C181S/C184S in 35mm dishes were treated with 15d-PGJ2-Biotin (5 µM) in DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin-streptomycin-Glutamine (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).
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bioRxiv - Biochemistry 2024Quote: Bacmids containing 6×His-MBP-TEV-nsp12 variants were generated using the Bac-to-Bac™ baculovirus expression system (Invitrogen). Sf9 cells were transfected with the bacmid DNA and baculovirus-containing cell supernatants were harvested 5 days after transfection ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: The region coding for the full-length Hermes transposase (Uniprot Q25438, with the mutations Q2E and K128G) was cloned into pBAD/Myc-His (Invitrogen) in such a way that the recombinant protein was not tagged ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Immunology 2023Quote: ... Both cell lines were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) and 100 U/mL penicillin-streptomycin (Gibco). Cells were incubated at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Microbiology 2022Quote: ... Infected Vero cell monolayers were immediately overlaid with DMEM 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500). R ...
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bioRxiv - Developmental Biology 2023Quote: Peaks enriched in neural crest samples were cloned from genomic DNA using standard PCR methods using AccuPrime Hi-Fidelity Taq polymerase (Invitrogen). Primers used to amplify target enhancer sequences are provided in the Key Resources table (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
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bioRxiv - Microbiology 2023Quote: ... All host defense systems identified in the various screens were placed under the araBAD promoter (arabinose inducible) in the pBAD Myc/His A vector (Invitrogen). Conjugative allelic exchange was carried out using R6K-origin pKEK2201 (kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... and restriction enzyme sites for NotI and EcoRV were introduced for cloning into the pcDNA4 myc/His backbone (Thermo Fisher). For NARS2-Apex2 cloning ...
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bioRxiv - Neuroscience 2023Quote: ... respectively) were amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Fragments of genomic DNA upstream of the first methionine codon (2677 bp ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were then separated by SDS-PAGE gels and transferred to the PVDF membrane for either immunoblotting with His antibody (Invitrogen) or staining with Ponceau S ...
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bioRxiv - Biochemistry 2024Quote: ... His-Smt3-Cysless GG-FAT10 (FAT10C0) was gene synthesized and subcloned into a pET28 vector by GeneArt (Thermo Fisher Scientific), the mutations are C7T ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted reaction was then transferred to a tube containing 80 µg of pre-washed Dynabeads His-Tag Isolation and Pulldown magnetic beads (Invitrogen) and incubated on a roller for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... and DsRed were cloned into pcDNA6 vector between the XhoI and XbaI sites (pcDNA6/Myc-His B; Invitrogen, Waltham, MA) with a poly(A)-tail of 84 nucleotides (Wakai et al. ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: The human anti-DNP and anti-WTA GlcNAc-β-4497 monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (21) ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Other monoclonal human anti-DNP and anti-WTA IgG of the four IgG subclasses were produced recombinantly in human Expi293F cells (Life Technologies) as described before 51 with minor changes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
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bioRxiv - Cell Biology 2023Quote: HCT116 human colorectal cancer and SUIT-2 human pancreatic ductal adenocarcinoma cells were grown in McCoy’s 5A medium (Gibco, Cat. # 26600080) supplemented with 10% v/v dialyzed Foetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell binding experiment was done with the human T lymphocyte cell Jurkat and anti-human IgG antibody-Ax488 (ThermoFisher Scientific). Ax488 signal changes upon binding were detected using a flow cytometer (BD Accuri C6 ...
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bioRxiv - Immunology 2023Quote: ... Human serum used for all conditions was from the same lot of pooled human AB serum (Fisher Scientific, Cat#: BP2525-100). For heat inactivation ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.