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Citations for Thermo Fisher :
3551 - 3600 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... Toehold strands were diluted to 1011 strands and mixed with biotinylated oligos at a ratio of 1:40 in a 50 µL reaction containing 2 mM MgCl2 (Invitrogen, Y02016) and 50 mM KCl (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... Retrograde labeling of ascending and descending spinal cord neurons located in the spinal segment 15 was performed using dye injections with biotinylated dextran (3000 MW; ThermoFisher, D7135) into segments 10 or 20 respectively ...
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bioRxiv - Cell Biology 2020Quote: ... both cell lines were lysed and biotinylated proteins were isolated using Dynabeads™ MyOne™ Streptavidin C1 (65002, Thermo Fisher Scientific) following the protocol described elsewhere (Roux et al ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of biotinylated S1 protein (Genscripts) was used to saturate the binding sites on 1 µl of FluoroSphere neutravidin beads (Thermo Fisher) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Cleared lysates containing biotinylated proteins in modified RIPA buffer were incubated with 30 µL of pre-washed streptavidin-coated magnetic beads (Thermo Fisher) for 3 hours at 4°C whilst rotating ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein fractions were site-specifically biotinylated using the BirA (Acro Biosystems Inc.) and subjected to a batch purification step with glutathione agarose (ThermoFisher Scientific) to remove the BirA ligase and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were subsequently washed in PBS-T three times and incubated in PBS-T containing 1:200 biotinylated goat anti-mouse IgG (Molecular Probes) as the secondary antibody at 25 °C for 1 day ...
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bioRxiv - Plant Biology 2021Quote: ... the purified protein was mixed with the appropriate concentration of each auxin before passing it over a streptavidin chip loaded with biotinylated SoIAA2 and SoIAA2Δ9 degron peptides (Thermo Fisher Scientific) (Supplementary Table 3).
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... SARS-CoV-2 S trimer protein was biotinylated using the EZ-Link(tm) Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher) and then purified using Zeba(tm ...
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bioRxiv - Immunology 2022Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μl of streptavidin magnetic beads were incubated with 4 μg of anti-CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, #13-1719-82, Thermo Fisher) for three hours on ice ...
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed 3 times with PBS-T and incubated with appropriate fluorophore-conjugated or streptavidine-fluorophore conjugated (for APOE biotinylated antibody) secondary antibodies (1:500, Life Technologies) together with nuclear stain Hoechst 33342 (1:2000 ...
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bioRxiv - Molecular Biology 2020Quote: The lysate and purified proteins were incubated with 3 µg of biotinylated RNA probe coupled to 20 µl Dynabeads MyOne Streptavidin C1 (Thermo Fisher) in 600 µl lysis buffer at 4°C for 1 hour rotating head-over-tail ...
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bioRxiv - Neuroscience 2021Quote: ... using 100 μg custom monoclonal anti-GFP antibodies (50 μg of clone 19C8 with 50 μg of clone 19F7) bound to biotinylated Protein L (Pierce, Thermo Fisher) coated streptavidin-coated magnetic beads (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 mg of these biotinylated lysates were adjusted to 500 μl and applied to 40 µl prewashed streptavidin-agarose beads (Thermo Scientific) and rotated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Typhimurium was grown in BHI broth until OD600:1 and then biotinylated with EZ-link NHS-Biotin reagent (Thermo Fisher Scientific). After washing ...
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bioRxiv - Biophysics 2021Quote: ... The liposomes containing fluorophore-labeled hHv1 channels were retained on the PEGlyated surface coated with biotinylated Histag antibodies (1:200 dilution, ThermoFisher Inc.). Fluorophores were excited by 532 nm laser (∼1.0 W/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated for 1 h at room temperature with secondary biotinylated antibodies or with the ABC kit (Cat. no. 32020, Thermo Fisher). SuperSignal West Femto (Thermo Scientific Cat n ...
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bioRxiv - Microbiology 2022Quote: 100 µl of purified and trypsin activated biotinylated rRV/VP4-BAP was incubated with 1 µl of streptavidin-Alexa Fluor 555 (2mg/ml) (ThermoFisher Scientific) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Poly-A selection was used to obtain only mRNA using poly-A biotinylated beads as per instructions (Dyna-beads Life technologies). The mRNA was used in an RT reaction that was specifically selected for the barcoded mRNA (Transcriptor High Fidelity Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... Half of the isolated total RNA underwent DNase treatment and a mixture of three GFP-specific biotinylated primers (#120, #123 and #126)(Supplementary Table 7a) were used to capture GFP transcripts with Streptavidin C1 Dynabeads (Life Technologies). An additional DNase treatment was performed ...
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bioRxiv - Bioengineering 2019Quote: ... the GVs were biotinylated by incubating them for 1 hour with a 10,000-fold molar excess of sulfo-NHS-biotin (Thermo Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... supernatants were incubated at 4°C with end-over-end rotation for 16-18 hours with biotinylated Streptavidin T1 Dynabeads (ThermoFisher, 65601) previously conjugated with GFP antibodies (Sloan Kettering Institute Antibody Core ...
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bioRxiv - Biochemistry 2020Quote: ... clarified supernatants were incubated with 30μl high-capacity neutravidin agarose beads overnight to capture biotinylated proteins (Thermo Scientific, catalogue number 29204). Beads were then washed 3x with ice-cold RIPA buffer and eluted with 2x SDS sample buffer with excess biotin at 95°C for 15 mins ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated cRNA targets were prepared from 250 ng of total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, ThermoFisher Scientific). Labelled cRNA (750 ng ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated cRNA targets were prepared from 250 ng of total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, ThermoFisher Scientific). Labelled cRNA (750 ng ...
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bioRxiv - Neuroscience 2019Quote: ... courtesy of Peter Davies, Albert Einstein College of Medicine), biotinylated anti-phospho tauSer202/Thr205 AT8 (mouse, 1:500) (Thermo Scientific, USA) or anti-human tau CP27 (mouse ...
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bioRxiv - Molecular Biology 2021Quote: The biotinylated pre-mRNA (10 pmol) was immobilized with 5 µL of Dynabeads MyOne StreptavidinT1 magnetic beads (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: The biotinylated pre-mRNA (10 pmol) was immobilized with 5 µL of Dynabeads MyOne StreptavidinT1 magnetic beads (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4◦C with 100 µl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was biotinylated by incubating cells for 10 min with 1 mg of EZ-Link-Sulfo-NHS-LC-biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Immunology 2020Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4°C with 100 μl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was then biotinylated by incubating cells for 3 min with 30 mg of EZ-Link-Sulfo-NHS-LC-Biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Cell Biology 2021Quote: ... The biotinylated DNA was pulled down by 10 μL of 10 mg/ml Dynabeads MyOne Streptavidin C1 beads (Life technologies, 65001). The beads were then re-suspended in 23 μL of 10 mM Tris-Cl pH 8.0 and libraries were prepared by on-bead reactions using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB ...
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bioRxiv - Immunology 2021Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the lysates was measured via BCA assay and biotinylated proteins were immunoprecipitated using Streptavidin magnetic beads (Thermo Fisher). 500μg magnetic beads were incubated with up to 70μg of lysate rotating overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Ferric Hb and ferrous Hb were individually biotinylated using sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6- {biotin-amido} hexanoate; Thermo Fisher Scientific) and immobilized on streptavidin beads in spin columns (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Secondary staining’s were performed for 1 h at room temperature in the dark with biotinylated goat anti-rabbit IgG AlexaFluor 405 (1:400; A-31556, Life Technologies) and goat anti-mouse IgG1 AlexaFluor 647 (1:800 ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated PR8-HA and S proteins were fluorescently labelled by the sequential addition of streptavidin-conjugated to phycoerythrin (PE) (Thermo Fisher) prior to use.
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bioRxiv - Immunology 2021Quote: ... Protein labelled cells were stained with biotinylated SARS-CoV-2 S1 (ACRO biosystems – S1N-C82E8) and detected with streptavidin AF647 (Invitrogen – S21374). Cells were stained with 7-AAD viability staining solution at 5 μl/1×106 cells to determine live cells ...
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bioRxiv - Biochemistry 2022Quote: Purified protein at a concentration of 1 mg/ml was chemically biotinylated using EZ link NHS-PEG4-Biotin (Thermo Scientific, A39259) at a 10-30 times molar excess ...
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bioRxiv - Biophysics 2022Quote: ... Briefly, ligands (DinB, DinBΔPAD, and DinB-PAD) were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly dissolved in H2O ...
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bioRxiv - Physiology 2022Quote: ... Immunoreactivity of the anti-VGAT rabbit antibody was detected by incubation with biotinylated donkey antibody to rabbit IgG followed by incubation with streptavidin-conjugated Alexa647 (1:400; S21374, Thermo Fisher).
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bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in DMEM/F12 medium and incubated with biotinylated EGF conjugated with Alexa Fluor 647-streptavidin (EGF-647; 2 μg/ml; Life Technologies) for 30 to 40 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated with biotinylated secondary antibody and developed with HRP and substrate using a DAB substrate kit (Thermo Fisher Scientific). Primary and secondary antibodies used in this study are listed in Supplemental Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... were processed to generate amplified and biotinylated sense-strand cDNA targets using the GeneChip® WT PLUS Reagent kit from Affymetrix according to the manufacturer’s specifications ...