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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... the resulting traction force gels were coated with 30 ug/ml collagen type I using sulfo-SANPAH (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... individual testes were macerated through 70-µm cell strainers and digested with 0.05% collagenase type IV (Thermo Fisher Scientific) in RPMI 1640 medium for 30 mins at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the DNA concentration of all sample types was measured with Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA).
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bioRxiv - Cell Biology 2023Quote: A549 (CCL-185) cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM (Gibco, C11995500BT) with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... wild type HEK293 or HEK293A (for figure 1A) cells were transfected with the indicated plasmids with Lipofectamine2000 Reagent (Invitrogen) as per manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... These tissues were chopped and digested at 37 °C in a Collagenase Type IV (150U/ml) (Cat 17104019-Gibco) and DNase I (60U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... E18 wild-type mouse primary cortical neurons were extracted as previously described and cultured in neurobasal medium (Invitrogen, #21103049) supplemented with 2% B-27 supplement (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from mating wild-type and EMA2 KO cells were isolated at 6 hpm using TRIzol reagent (Invitrogen). The RNA was treated with Turbo DNase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: Wild-type and cleavage deficient (dominant negative) TFIIS with mutations D190A and E191A were cloned into the pCDNA3.1 (Invitrogen) plasmid for mammalian expression ...
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bioRxiv - Cancer Biology 2023Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/mL [GIBCO], Dispase 5 mg/mL [GIBCO] ...
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bioRxiv - Bioengineering 2023Quote: ... HeLa and MDA-MB-231 cell lines were purchased from the American Type Culture Collection (ATTC) and cultured in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: The full-length wild-type human NTCP gene (UniProt ID: Q14973) was generated synthetically by GeneArt (Thermo Fisher Scientific) after codon optimization for expression in HEK293 cells ...
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bioRxiv - Cell Biology 2023Quote: ... Postnatal and adult mouse tissues were first digested for 20min at 37°C with 2.5 mg/mL collagenase type I (Thermofisher), 2.5 mg/mL dispase II (Thermofisher) ...
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bioRxiv - Bioengineering 2022Quote: ... untreated polymer bottom slides were used followed by bovine Type 1 collagen coating (50 μg/ml, A10644-01, Gibco). For the soft and medium stiffness groups ...
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bioRxiv - Cell Biology 2022Quote: ... tissue was digested for a further 24 h with 0.5 mg/mL collagenase type IV (CLS-4, Lorne Laboratories) and 1 mg/mL dispase II (17105041, Invitrogen) at 37 °C and 5% CO2 with constant agitation [38].
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bioRxiv - Immunology 2022Quote: ... and MDA5-/-/RIG-I-/- double knockouts) or wild type cells were transfected with Silencer Select small interfering RNAs (Ambion) targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’ ...
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bioRxiv - Developmental Biology 2022Quote: ... 60 μL of organoid digest buffer (Dispase [Corning, 354235, undiluted, 50 U/mL], DNase I [GoldBio, D-301, final concentration 0.25mg/mL], Collagenase Type I [Gibco, 17100017 ...
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bioRxiv - Genomics 2022Quote: ... DNA samples were adjusted to 80 ng/μL and digested using the IIb type restriction enzyme AlfI (Thermo Fisher). Specific adaptors and individual sample barcodes were ligated and the resulting fragments amplified ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates had been pre-coated with 12.5 μg/cm2 rat-tail collagen type I (Gibco, ThermoFisher Scientific, Perth, UK). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... lungs were cut into small pieces and digested in RPMI solution containing collagenase type 1 (ThermoFisher Scientific Waltham, MA) for 1 hr at 37°C in an agitating incubator ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from wild type HeLa cells and UbKEKS knockout clones 4.3 and 2.7 cells using TRIzol reagent (Invitrogen, USA) and reverse transcription was performed using ProtoScript II reverse transcriptase (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... we isolated total protein from 45 dpf adad1Y67X mutant and wild-type testes in RIPA buffer (Thermo Fisher Scientific). After denaturation through heat and 100 mM DTT ...
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bioRxiv - Developmental Biology 2022Quote: ... the tissues were digested with dissociation buffer including 1 mg/mL type IV collagenase (Gibco, Grand Island, NY, USA) and 200 μg/mL DNase I (Roche ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... period (per) and tr-cryptochrome (tr-cry aka mammalian-type cry) via real-time qPCR (StepOnePlusTM, Applied Biosystems, USA) using previously established primers [28] and cell division cycle 5 (cdc5 ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pellet was then resuspended in digestion solution containing 2mg/ml Collagenase Type 2 (17101015, Thermo Fisher Scientific) in DMEM (41966029 ...
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bioRxiv - Cell Biology 2024Quote: ... PAA gels were washed gently once with PBS followed by coating with 100 μg/ml collagen type I (Gibco) in PBS overnight at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Strains with the chromosomal mutations were grown similarly in N-minimal media containing 0.01 mM Mg2+ until wild-type Salmonella reached OD600=0.3 and extracted total RNA using Tri reagent (Ambion, 15596018). 10 µg of RNA samples were mixed to an equal volume of RNA sample loading buffer (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: Caco-2 (HB-37) cells were purchased from the American Type Culture Collection (ATCC) and cultured in DMEM (Gibco) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2024Quote: Vero E6 cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... The same culture medium base formulation and conditions were used for all of the above cell types: DMEM (Gibco) supplemented with 10% v/v fetal bovine serum and 1% v/v antibiotic-antimycotic (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The 3xHA-NTSR1 plasmid consists of N-terminal 3xHA-tagged wild-type human NTSR1 cloned into the pcDNA3.1(+) vector (Invitrogen, Thermo Fisher Scientific Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... dispase type II – 4.7 mg/ml in Hank’s balanced salt solution Mg2+ and Ca2+ free (HBSS-GibcoTM ThermoFisher Scientific)) ...
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bioRxiv - Immunology 2024Quote: ... depending on the cell type or activation and incubated for 30 min at 4° C in HBSS (ThermoFisher Scientific) Stimulation media (HBSS with Ca2+ and Mg2+ ...
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bioRxiv - Pathology 2024Quote: ... FACS-sorted HSCs from the liver tissues of C57BL/6 wild-type female mice were cultured in the Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, #C11995500BT) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2024Quote: Wild-type PCC 7002 was inoculated into 25 mL Media A+ (initial OD730 = 0.05; Thermo Scientific Genesys 10S Vis) and grown overnight in the Erlenmeyer flask format until an OD730 of 0.2-0.3 was reached ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were transfected with 50 ng per well of pcDNA3.1+ mock vector or pcDNA3.1+ myc-TIRMyD88 (wild-type or mutants) in 96-well plates using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Oocytes were then injected with 50 nL of 200 ng/µL full-length wild-type or mutant hP2X4 mRNA synthesized from pCDNA3.1x using the mMessage mMachine T7 Ultra Kit (Invitrogen). The oocytes were incubated at 18 °C for 2 days to allow sufficient receptor expression ...
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bioRxiv - Cell Biology 2024Quote: Ea.hy926 cell line was purchased form the American Type Culture Collection (CRL-2922) and grown in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, Thermo Fisher Scientific Inc.) and blotted onto nitrocellulose (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, Thermo Fisher Scientific Inc.) and blotted onto nitrocellulose (926-31092 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with N2 (made in-house 2x stock in Advanced DMEM/F-12 (Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2020Quote: ... and hTERT-RPE-1 Cyclin A2-eYFP54 were cultured with DMEM/F-12 + GlutaMax (Life Technologies), and BJ primary human foreskin fibroblasts (a kind gift from Bennie Lemmens ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was performed on BOLTTM Bis-Tris Pure 4-12% gradient gels (Thermo Fisher Scientific) in MES-SDS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... PC-12 cells were grown in DMEM (high glucose, pyruvate included, Thermo-Fisher, Gibco™, 11995073) with 5% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured in DMEM/F-12 supplemented with 2.5 mM L-glutamine (Gibco, Carlsbad, CA, USA) and 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... and Adgrg6 KO ATDC5 cells were maintained in DMEM/F-12 (1:1) medium (Gibco, 11330032) supplemented with 5% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates were resolved under denaturing conditions by SDS-PAGE (4–12% Bis-Tris gels; Invitrogen) and transferred to nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: In vitro kinase reactions were separated on 4-12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific) and gel bands were visualized with SimplyBlue™ SafeStain (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... and flat-mounted onto microscope slides (Superfrost/Plus, 12-550-15; Thermo Fisher Scientific, Waltham, MA) with photoreceptor side down and embedded in antifade reagent (SlowFade ...