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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Cell lysates were separated by SDS-PAGE (4-12% Bis-Tris gels; Thermo Fisher Scientific) under reducing conditions and transferred onto nitrocellulose membranes (Cytiva) ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 5-10µg of lysate was ran on 4-12% NuPAGE Bis-Tris Gels (ThermoFisher), transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... and SK-N-SH cell lines were purchased from the American Type Culture Collection (www.atcc.org) and grown in a humidified chamber with 5% CO2 in RPMI 1640 medium (Gibco, #11875119) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... 15 cm plates of cells were transfected with 10 ug DNA (pcDNA 3.1 plasmids encoding wild-type FLAG-RIPK2 or K209R FLAG-RIPK2) using Lipofectamine 2000 (Invitrogen) at a 1:1 ratio of reagent:DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... decapsulated and transferred in low-binding Eppendorf tube containing 100-150µL collagenase solution in HBSS (Collagenase type IV (Gibco), 2X AAs (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... Wild type and Ex6KO cell lines were plated on a 6-well plastic plate (Nunclon Delta Surface,ThermoFisher Scientific). When confluent ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
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bioRxiv - Immunology 2020Quote: ... NINJA-F and wild-type B6 mice were bled retro-orbitally using heparinized glass capillaries (Fisher Scientific, cat. 22362566) to collect 50-100uL of blood into 0.04% Citrate Buffer ...
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bioRxiv - Bioengineering 2020Quote: ... plasmids bearing wild type HSA and the mutants were transfected to HeLa cells using Lipofectamine 3000 transfection kit (Invitrogen) and Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... Yvonne Chen) and wild-type K562s (acquired from Dr. Krishnendu Roy) were cultured in Isocove’s Modified Dulbecco’s Medium (ThermoFisher #12440053) supplemented with 10% FBS (Fisher #16140071 ...
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bioRxiv - Biochemistry 2021Quote: ... Wild type/native human basigin (BSG-2, residues M1-L206) was expressed in Expi293™ cells (Thermo Fisher Scientific) with C-terminal rat CD4 domains 3+4 (CD4d3+4 ...
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bioRxiv - Physiology 2020Quote: ... adipose tissue was allowed to recover in KRBH buffer containing 2.5% BSA for 20 min before being chopped into tiny pieces and incubated in KRBH buffer containing 0.5% BSA and 1% collagenase (type II; #17101015, Gibco) for one hour in a 37 °C shaking water bath ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was isolated from the various organs of birg1 and wild-type plants using Trizol reagent (Life Technologies). About 1 μg RNA was reversely transcribed using oligo (dT ...
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bioRxiv - Systems Biology 2020Quote: ... Plasmids containing the expression cassettes containing the wild type and mutant bla gene were transformed into Top10 cells (Invitrogen) and treated with various Amp concentrations as described above.
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bioRxiv - Cancer Biology 2022Quote: A549 cell line was purchased from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, USA), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: Wild type mouse liver RNA was isolated with RNA TRIzol™ Reagent (Ambion by life technologies, Cat No: 15596018) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/ml [GIBCO], Dispase 5 mg/ml [GIBCO] ...
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bioRxiv - Cell Biology 2022Quote: HEK 293T cells were transfected with 1 µg of plasmids containing either FL (wild-type), various deletions (ΔTM, ΔNΔTM, NCC, CC, N) or mutant FLAG-KASH5 constructs with Lipofectamine LTX (Invitrogen) transfection reagent using the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: The wild-type HeLa cells transiently expressing amyloid (Q103-GFP) aggregates were cultured in DMEM (31966-021, Thermo Fisher), high glucose with GlutaMax medium (61965-026 ...
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bioRxiv - Bioengineering 2022Quote: ... the recellularized liver after perfusion culture was incubated with RPMI (Wako) containing 10 mg/mL collagenase type II (Gibco) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Respective wild-type and mutant subunit cRNAs were synthesized in vitro with the mMessage mMachine T7 transcription kit (Invitrogen), titrated and checked for integrity ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Cell Biology 2020Quote: PDGFRα+ FAPs were FACS-isolated from either wild-type or PDGFRαH2BEGFP/+ mice and grown in high-glucose DMEM (Invitrogen), supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... mouse cortical precursor cells from cortices were dissected from E12.5 wild-type or Dhcr7-KO mouse embryos in ice-cold HBSS (Invitrogen) and transferred into cortical precursor medium ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells were obtained from the American Type Culture Collection (ATCC) (catalogue number CRL-1573, RRID:CVCL_0045) and were cultured in minimal essential medium (MEM) (Life Technologies) containing 10% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... after which an additional 440 U of Type 1A collagenase was added along with Dispase II (Life Technologies, #17105041) and DNAse I (Bio Basic ...
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bioRxiv - Genomics 2022Quote: ... Cardiac tissue pieces were then digested in tissue dissociation media with 200U/mL collagenase type II (Gibco 17100-015), 1 mg/ml dispase (Sigma D4693) ...
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bioRxiv - Genomics 2022Quote: ... These pieces were then transferred to fresh tubes and incubated in 200U/ml Collagenase type I (Gibco 17100-017) and 3mM calcium chloride in PBS for three cycles of 10 minutes under mild agitation at 37°C in 1.5 ml eppendorf tubes ...
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bioRxiv - Developmental Biology 2020Quote: ... The MCF10A cell line was obtained from the American Type Culture Collection and were maintained in DMEM/F12 (Gibco), 5% Horse Serum (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: CAFs were isolated from human colon carcinoma resection specimens following 2.4U/ml dispase + 100U/ml collagenase type II (both from Invitrogen); disaggregation of tumour fragments and digestion for 2h t 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... were obtained from the American Type Culture Collection (ATCC, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA) supplemented with 1% penicillin-streptomycin and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were immersed in 2ml dissociation solution (2% FCS-PBS solution with approximate 145U/ml type 4 Gibco collagenase) and incubated at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Lateral skin explants were taken from E13.5 wild-type embryos and placed on Nucleopore Track-Etch membranes (Whatman) floating on DMEM (Gibco) containing 10 % FBS and 1x antibiotic/antimycotic solution (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... Neuro-2a cells were transfected with wild-type (WT) or mutant (MUT) reporter plasmids by using Lipofectamine 3000 (Invitrogen). At 24 hours post transfection ...
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bioRxiv - Genomics 2021Quote: The human immortalized normal renal cell line HEK 293T was obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Immunology 2020Quote: ... bone marrow cells were flushed from femurs of lipin-1 fl/fl (wild type) and lipin-1m KO mice with Dulbecco’s modified Eagle’s medium (DMEM; Gibco, 10829). Cells were centrifuged at 534xg for 5 minutes ...
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bioRxiv - Developmental Biology 2020Quote: The ChIP assay was performed on wild type and Hes1 GOF E11.5 limb buds using the MAGnify Chromatin Immunoprecipitation system (Invitrogen) using the protocol described (Rutkowski et al ...
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bioRxiv - Developmental Biology 2020Quote: Whole and anterior limb buds at E11.5 were isolated from wild type and Hes1 GOF embryos and lysed in RIPA buffer (Invitrogen) containing protease inhibitors ...
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bioRxiv - Biophysics 2023Quote: U937 and HCT116 (p53 wild type or null) cells were cultured in RMPI 1640 medium (11875, Thermo Fisher Scientific) supplemented with 10% FBS and 1% PS ...
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bioRxiv - Biophysics 2023Quote: ... and 0.25 μg of wild-type or mutant UL34 plasmid using Lipofectamine as described by the manufacturer (Gibco-BRL) and incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... GBP1 wild-type and mutants were labeled as described above with AlexaFluor647-C2-maleimide dye (AF647, Thermo Fisher Scientific). Labeling was performed at an equimolar ratio of protein and dye on ice for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... 105 cells were seeded onto 1 μm pore polyester membrane inserts (Falcon) previously coated with type I collagen (Gibco). Once cells reached full confluency ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were minced and then digested with 100U/ml of Collagenase Type II (Gibco, Life Technologies, Carlsbad, CA, USA) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were minced and then digested with 100U/ml of Collagenase Type II (Gibco, Life Technologies, Carlsbad, CA, USA) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... was purchased from American Type Cell Collection (ATCC) and cultured in eagle’s minimal essential medium (MEM) supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin and L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: The clinical tissue specimens were digested into single cells with 0.5 mg/ml Collagenase Type IV (Thermo Fisher Scientific) in DMEM containing 25 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates had been pre-coated with 12.5 μg/cm2 rat-tail collagen type I (Gibco, ThermoFisher Scientific, Perth, UK). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids for wild-type or mutant PAC channels was transiently transfected into HEK293T/PAC-/- cells using Lipofectamine per the manufacturer’s instructions (Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: 30 hpf MZ nkx3.1ca116 mutant and wild type embryos were collected and RNA prepared using Trizol (ThermoFisher, Waltham MA). Libraries were prepared from 50ng/µg/µl of total mRNA per sample using the Illumina Ultra II directional RNA library prep (SanDiego ...