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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for lefty A TGF beta 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Wild-type human HCAR2 was cloned into pFastBac vector (Gibco) with N-terminal hemagglutinin (HA ...
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bioRxiv - Cancer Biology 2023Quote: ... and rat anti-human CD44 (1:50, clone IM7, Invitrogen). The secondary antibodies included alexa fluor 488 labelled donkey anti-mouse (xxx ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 Human Uncoated ELISA Kit (88-7025, Invitrogen) were used according to the manufacturer’s protocol and the signal was obtained in a microplate reader (Sunrise ...
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bioRxiv - Immunology 2023Quote: ... Human HLA-ABC (W6/32) – PE was purchased from Invitrogen. Human CD159a (REA110 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 ng/mL human rGM-CSF (PHC2013, Gibco) and 0.86 ng/mL human rIL4 (204-IL ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were analyzed using Human RANTES ELISA Kit (ThermoFisher, EHRNTS) and IL-1RA Human ELISA Kit (ThermoFisher ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Gibco Human Episomal iPSC clone (Control 1, Thermo Fisher Scientific) and CW30318 (Control 2 ...
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bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with Human CD11b-APC conjugate ( Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... They were cultured on human recombinant vitronectin (Thermo Fisher Scientific) coated plates in StemFlex medium (Thermo Fisher Scientific ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Anti-human CD45-PerCP-Cy5.5 (45-9459-42, Affymetrix eBioscience), CD45-APC Cy7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were obtained from ThermoFisher and maintained in Human Large Vessel Endothelial Cell Basal Medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... LAN5 human neuroblastoma cells were cultured in RPMI (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and human HEK293T cells was extracted using TRIzol reagent (Invitrogen). Endogenous RNase was further removed by ethanol precipitation ...
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bioRxiv - Cell Biology 2023Quote: ... HEK 293FT human embryonic kidney cells (Thermo Fisher Scientific, #R70007) were cultured in DMEM (Corning ...
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bioRxiv - Physiology 2024Quote: Human islets were cultured in CMRL medium (Gibco 11530-037) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs were purchased from Life Technologies (for human cell lines) and from Horizon Discovery (for human PCLS) ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 ng/mL recombinant human EGF (Gibco Cat# PHG0311).(22 ...
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bioRxiv - Genomics 2024Quote: 10 ng of fully methylated human blood (Thermo Scientific, SD1131) and Jurkat (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... human neutrophils were labeled with calcein AM (Thermo Fisher Scientific) and left to migrate for 2 hours before termination of the experiment ...
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bioRxiv - Bioengineering 2024Quote: ... Research-grade DynabeadsTM Human T-Expander CD3/CD28 beads (Gibco) were washed using DynaMag-2 magnet (Gibco ...
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bioRxiv - Synthetic Biology 2024Quote: Rabbit polyclonal anti-Human ASGR1 antibody (Thermo Fisher, PA5-80356), 1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 ng/ml human bFGF (PHG0021, Thermo Fisher Scientific)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant Human EGF (Invitrogen, Cat#PHG0313, 100 µg/500 mL), Hydrocortisone (Sigma ...
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bioRxiv - Immunology 2024Quote: ... goat anti human IgG (H+L) HRP (Invitrogen, Cat#31412) was added to wells at a 1:10,000 dilution for one hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: Gibco Human Astrocytes (cat. nr. K1884, Lot nr. 802268, Invitrogen) were sourced from a 20 weeks old male donor ...
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bioRxiv - Cell Biology 2024Quote: ... A human IL-6 ELISA kit (Thermo Fisher Scientific, KHC0061) was then used as instructed by the manufacturer to measure IL-6 concentrations ...
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bioRxiv - Neuroscience 2024Quote: ... Luminex assay was performed using ProcartaPlex Human Basic Kit (Invitrogen) and a panel of ProcartaPlex Human Simplex (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed twice with human wash buffer (DMEM/F12 (Gibco), 10 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... or a human NT3 ELISA kit (Thermo Fisher Scientific, EHNTF3). Both ELISAs were performed as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CAFs were kept in Human Plasma-Like Medium (HPLM, Gibco) with ascorbic acid for 11 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 Units/mL human recombinant insulin (Gibco, #12585-014), seeded on plates coated with rat tail collagen (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... or anti-human IgG Alexa Fluor 647 conjugate (Invitrogen, A21445) or Streptavidin Alexa Fluor 647 conjugate (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads Human T-Activator CD3/CD28 (11161D; Gibco; 1:100) or plate bound with anti-CD3 and anti-CD28 antibodies (0.5µg/ml each ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human EGF (Thermo Fisher Scientific, #PHG0311, 50 ng/ml), N-acetylcysteine (Sigma Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
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bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
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bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
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bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
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bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...