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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for lefty A TGF beta 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 4 x 250 mm column (Thermo Scientific) and eluted using a gradient of sodium chloride in a phosphate buffered mobile phase ...
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bioRxiv - Developmental Biology 2023Quote: ... fixed with 4% paraformaldehyde (PFA) (Affymetrix/USB) in PBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... on a Qubit 4 fluorometer (Thermo Fisher) and the high sensitivity D1000 reagents and tapes (Agilent #5067-5585 ...
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bioRxiv - Cancer Biology 2023Quote: ... on a Qubit 4 fluorometer (Thermo Fisher) and the high sensitivity D1000 reagents and tapes (Agilent #5067-5585 ...
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bioRxiv - Cancer Biology 2023Quote: ... on a Qubit 4 fluorometer (Thermo Fisher) and the high sensitivity D1000 reagents and tapes (Agilent #5067-5585 ...
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bioRxiv - Neuroscience 2023Quote: ... 4 μl 5x SSIV Buffer (ThermoFisher, 18090010), 1 μl 0.1M DTT (Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... and methyl-(PEG)4-NHS (Life technologies) in 1 x PBS were introduced and incubated for 1 h [120] ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat ...
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bioRxiv - Cell Biology 2024Quote: ... paraformaldehyde (PFA, 4% vol/vol, ThermoFisher #28908) diluted in DPBS was introduced to the top and bottom channels and chips were incubated for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... fixed in 4% paraformaldehyde (Thermo Fisher Scientific) for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... on a Qubit 4 Fluorometer (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Fluo-4-Direct assay (ThermoFisher Scientific; USA) was utilised to measure intracellular calcium concentration in isolated mouse spinal cord pericytes plated in a 96 well plate ...
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bioRxiv - Biochemistry 2023Quote: ... and Qubit 4 Fluorometer (Thermo Fisher Scientific), respectively ...
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bioRxiv - Biophysics 2023Quote: ... and 4 uL RNaseA (Thermo Scientific; EN0531) in RNaseH buffer using the following program ...
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bioRxiv - Microbiology 2023Quote: ... 4 U of turbo DNase I (Ambion) was added (37°C ...
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bioRxiv - Immunology 2024Quote: ... 96-well Immuno 4 plates (Fisher Scientific) were coated with 10mg/ml goat anti-mouse Ig (Southern Biotechnology ...
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bioRxiv - Bioengineering 2023Quote: ... a 4% low melting point agarose (Invitrogen) solution was prepared in sterile 1X PBS and kept in a 37°C water bath ...
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bioRxiv - Microbiology 2024Quote: ... cells were fixed in 4% paraformaldehyde (Invitrogen) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 4% formaldehyde (Thermo Fisher, cat. BP531-500) was applied to cells for fixation for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: PFA 4% (ThermoFisher, cat. no. J19943.K2) ! CAUTION Very toxic and carcinogenic substance that may cause death or damage to health when inhaled ...
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bioRxiv - Biochemistry 2024Quote: ... 4–12% Bis-Tris gels (ThermoFisher, #NP0322) and run in 1X MOPS buffer (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Vitrobot Mark 4 (Thermo Fisher) at a relative humidity of 100% and 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and 4% sucrose (Fisher Scientific, S5-500) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... CD152 (CTLA-4) (Invitrogen, APC, clone 14D3), HLA-DR (BD ...
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bioRxiv - Neuroscience 2024Quote: ... with 4% fetal bovine serum (Gibco, 175004044) and the cortices were manually dissociated using a pipette ...
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bioRxiv - Genomics 2024Quote: ... 4% B27 supplement (ThermoFisher Scientific, #17504-044), 30 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were fixed in 4% formaldehyde (ThermoFisher) for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... eyes had 15µl of 4% formalin (ThermoFisher) dropped onto them and were left to fix at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and 4 μg/mL laminin (Life Technologies).
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bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Cancer Biology 2022Quote: ... HepG2 cells were fixed with 4% paraformaldehyde and then stored at 4°C in RPMI 1640 (Gibco, Cat# 11875119) media supplemented with 10% heat inactivated FBS ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... post-fixed in 4% paraformaldehyde and 30% sucrose for 4 days before being frozen in Neg50 OCT (Thermo Scientific) 30 micron sections were taken and stored in PBS with sodium azide at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Stock BODIPY505/515 was prepared by dissolving 4,-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene (Life Technologies, D3921) powder into dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed using a 4% PFA solution [0.2M sucrose | 4% paraformaldehyde solution in PBS (Thermo Fisher, J19943-K2)] ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4°C overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following synaptic antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Cell Biology 2019Quote: ... OCT4 and TRA1-60 overnight at 4°C using the pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Life Technologies), followed by incubation with an ALEXA secondary antibodies for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... 4 µl CFE reaction was loaded onto a NuPAGE 4–12% Bis–Tris Gel (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system ...
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bioRxiv - Immunology 2021Quote: Avi-tag-biotinylated spike and RBD were incubated with subsequent fluorochrome-labeled streptavidin at 4:1.5 ratio overnight at 4 °C as follows: spike with APC-streptavidin (Invitrogen), Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of cell suspension were applied and plunge-frozen using a Vitrobot Mark 4 system (Thermo Fisher Scientific) at blot force 10 ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...