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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Cell Biology 2023Quote: ... 25% of the total immunoprecipitated material (per condition) was loaded onto a 3-8% Tris-Acetate gel (Thermo Fisher), fully resolved gels were transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: ... The cytosol and membrane fractions were separated on the NuPAGE Novex Tris-acetate 3–8% gel (Thermo Fisher Scientific) or the PAG mini SuperSep Ace Tris-glycine 5–20% gel (FUJIFILM Wako Pure Chemical) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 95C and subjected to NuPage 3-8% Tris-Acetate gel (#EA0375PK2, Invitrogen). Separated proteins were transferred to cellulose membrane using a semi-dry transfer method and blocked in TBS-T with 5% dry milk containing 2% normal goat serum for 1hr ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked in 2% goat serum + 2% Bovine Serum Albumin for 2 hours at room temperature before primary antibody (GFP, 1:500, Invitrogen #A6455) was added and the embryos were incubated overnight at 4°C with rocking ...
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bioRxiv - Microbiology 2020Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher cat. A33952), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher, catalog #A33952), following the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Genomics 2020Quote: ... in in-house Essential 8 (E8) medium (DMEM Nutrient Mixture F-12 (Gibco) supplemented with 543mg/L NaHCO3 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... discoideum clones on SM/5 plates (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Genomics 2019Quote: ... 2 µL T4 DNA ligase (5 Weiss U/µL, Thermo Scientific) was added followed by incubation for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco, #PHC0027), 2 mM L-glutamine (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT Plus EdU Kit, Invitrogen) and 5-bromo-2’-deoxyuridine (BrdU ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µl of 2× concentrated PowerSYBR Green PCR MasterMix (Thermo Fisher), 2 pmoles of each primer and water up to 10 µl ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty min before fixation EdU (5-ethynyl-2’-deoxyuridine, Molecular probes) was added in all the experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^5 cells were lysed in RIPA buffer (Thermo Fisher) with protease and phosphatase inhibitors (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with EdU (5-ethynyl-2’-deoxyuridine, Life technologies) at final concentration of 10 µM for 4 hours and then harvest to perform Click-iT reaction using Click-iT EdU flow cytometry Alexa Fluor 488 assay kit (Life technologies ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 5 mM MgCl2 and 2% penicillin–streptavidin (Gibco, #15140122). Minced tissue was digested with enzyme solution at 37°C for 60-90 min with gentle shaking ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µM 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Carlsbad, CA, USA); 10 µM 5-bromo-2’-deoxyuridine (BrdU ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 5 mM MgCl2 and 2% penicillin–streptavidin (Gibco, #15140122). Tissue was incubated with enzyme solution for 30 min at 37°C with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) supplemented with 5 μM Fura-2 AM (Thermo Fisher), 50 μM pluronic acid F-127 (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: For combined 5-ethynyl-2-deoxyuridine (Invitrogen A10044, Thermo Fisher Scientific) and modified pseudo-Schiff-propidium iodide (PI ...
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bioRxiv - Plant Biology 2024Quote: For combined 5-ethynyl-2-deoxyuridine (Invitrogen A10044, Thermo Fisher Scientific) and modified pseudo-Schiff-propidium iodide (PI ...
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bioRxiv - Plant Biology 2024Quote: ... respectively were stained with EdU (5-ethynyl-2′-deoxyuridine; Thermo Fisher) and modified pseudo-Schiff propidium iodide (PI ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs targeting DHHC 2 and 5 were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2023Quote: ... 2% B-27 supplement and 5% fetal bovine serum (Invitrogen, Canada) plus 1/3 of minimum essential medium enriched with 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... mice received 50mg/kg 5-ethynyl-2’-deoxyuridine (EdU, Thermo Fisher) in PBS by intraperitoneal injection 24 hr before harvest ...
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bioRxiv - Cancer Biology 2023Quote: ... 40 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen, Waltham, MA, USA) was added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 5-ethynyl-2’-deoxyuridine (EdU; ThermoFisher A10044) dissolved in K-SFM (20 mM final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Cell Biology 2020Quote: ... Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C and 100% humidity.
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were digested with both trypsin and chymotrypsin and the resultant peptides were loaded onto a reverse phase C18 trap column (Acclaim PepMap 100, 75 μm x 2 cm, nano viper, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using an Ultimate 3000 and washed with 50 μL of solvent A at 10 μl/min ...
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bioRxiv - Neuroscience 2020Quote: ... CO slices were perfused at a rate of 2-3 mL/min in oxygenated MitoTracker staining solution containing: ACSF and MitoTracker™ Red CMXRos (Invitrogen™, M7512, 100nM) for 30 minutes at 29–32°C in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... and an Acclaim PepMap™ 100 trap column (2 cm× 75 μm inner diameter) containing C18 resin (3 μm, 100 Å; Thermo Fisher Scientific). The mobile phase consisted of 0.1% formic acid in ultrapure water (buffer A) ...
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bioRxiv - Cell Biology 2021Quote: ... We isolated neonatal cardiomyocyte (postnataTl 2 – 3 days) from Gja1M213L/M213L and Wildtype (WT) mice using PierceTM Primary Cardiomyocyte Isolation Kit (Thermo Fisher Scientific, Walthman, MA) following manufacturer protocol ...
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bioRxiv - Physiology 2023Quote: ... HBMECs were washed by 2% BSA (3×, 5min) followed by secondary antibody incubation with either Alexa Fluor 488 goat anti-mouse (ThermoFisher Scientific; Catalogue number: A11001) or Alexa Fluor 555 goat anti-rabbit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Brains were quickly transferred to 10 μL of the same saline in a 200 μL microcentrifuge tube kept at 2-3°C in a Boekel Scientific TropiCooler Benchtop Incubator (model 260014, Fisher Scientific, Ottawa, ON, Canada), and once 30 brains were collected (2-2.5 min per brain ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Immunology 2024Quote: Vero-CCL81 [for MuV and VSV_RUBV] or Vero-hSLAM [for MeV-IC323] target cells which were seeded at a density of 2 × 104 cells per well in flat-bottom 96-well plates (Fisher Scientific, #08-772-3). The cells were incubated at 37 °C/5% CO2 overnight (∼20 hours) ...
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bioRxiv - Neuroscience 2023Quote: ... and IVH-PHH (Supplementary Figure 2) P7 rats underwent intraventricular injection of 20 µl of 3 kDa Red Dextran Tetramethyl rhodamine (RD/TMR) (D3307, Thermo Fisher Scientific, Waltham, MA), 1.9-nm (1102 ...
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bioRxiv - Biochemistry 2023Quote: ... and ∼5-10 µL of peptide was loaded onto a reverse-phase trap column (Acclaim PepMap 100, 75 µm × 2 cm, NanoViper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, USA) using an Ultimate 3000 autosampler and washed with 40 µL of buffer A at a flow rate of 20 µL min−1 ...
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bioRxiv - Systems Biology 2024Quote: ... 2-3 butanediol, and erythritol), and organic acids (acetate, succinate, tartrate, citrate, and malate) were determined using an HPLC (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and UV/VIS (210nm ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... tissues were washed in PBS (3×5 min) and incubated with secondary antibodies conjugated to Alexa Fluor 488 (1:400; goat anti–rabbit; Life Technologies) or Alexa Fluor 594 (1:400 ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 µl of 3 ng µl-1 cDNA was used as template in the QuantStudio 5 Real-Time PCR system (Applied Biosystems). Amplifications were performed with 5 μl of SYBR® green JumpStart Taq ReadyMix (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Slices were then washed in PBS (3 × 10 min) followed by a 5 min incubation with DAPI (Thermofisher Scientific, 1: 5000) diluted in PBS ...