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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for Anti SUPT5H Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... or monoclonal mouse antibodies directed against TBEV E [21] and conjugated secondary antibodies anti-rabbit Alexa555 (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... the primary antibody was washed out and goat anti-mouse IgG (H+L) antibody conjugated with Alexa fluor 568 (Invitrogen; A11031) was added (1:1000 in PBS with Alexa Fluor 647 Phalloidin (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Anti-rabbit Alexa Fluor 488-conjugated secondary antibody (cat. No. A11008) and Anti-mouse Alexa Fluor 488 conjugated secondary antibody (cat no. A10680) were obtained from Thermo Fisher Scientific.
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Physiology 2022Quote: ... immunoreactivity for mCherry was detected by anti-mRFP guinea pig antibody and Alexa594-conjugated goat antibody to guinea pig IgG (10 μg/ml; A11076, Thermo Fisher).
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bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher) before neurons were washed 3 times with HBS and mounted on the microscope in HBS ...
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bioRxiv - Physiology 2022Quote: Primary antibodies were detected using the following secondary antibodies: Alexa Fluor 568 goat anti-chicken (Thermo Fisher Scientific, A11041, 1:1000), Alexa Fluor 594 goat anti-rabbit (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with a secondary antibody solution containing secondary antibody (anti-Guinea Pig IgG Alexa Fluor™ 488, A11073, Invitrogen, 1:500), 1% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488 conjugated rabbit anti-sheep antibody (Jackson Immu-noResearch 313-545-045) for colocalization experiments or Alexa Fluor 488 conjugated anti-mouse antibody (Invitrogen 21042) for GFAP immunostaining in 5% BSA for 1 hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Foci development was stopped by fixation with 4% formaldehyde and foci were then stained using a mouse-anti-NS1 antibody (1A5) (gift from Jacob Schlesinger, Rochester University) and a horseradish peroxidase (HRP) conjugated secondary anti-mouse antibody (ThermoFisher 31430). The foci were visualized by diaminobenzidine (DAB ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequently incubated for 30 min with secondary antibodies (Alexa Fluor goat anti-mousse IgG (H+L)/Alexa Fluor 647 secondary antibody (Invitrogen, A21236) at 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... coverslips were washed three times for 10 min in PBS and subsequently incubated with the secondary antibody goat anti-rabbit alexa-488 antibody (Thermo Fisher/Invitrogen) (1:300) ...
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bioRxiv - Plant Biology 2024Quote: ... as the primary antibody at 1:100 dilution and donkey anti-rabbit AlexaFluor 555 antibodies (A31572, Thermo Fisher Scientific, Waltham, MA) as the secondary antibody at 1:1000 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... DIG present on the synthesized mRNA probes was subsequently labeled with anti-DIG primary antibody (Enzo; ENZ-ABS302-0001; 1:1,000) and biotin-conjugated secondary antibody (Invitrogen; 1:500). To detect fluorescent signals ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used are: ECL anti-mouse immunoglobulin G (IgG) horseradish peroxidase linked whole antibody (1/10 000; Fisher Scientific; NXA931V) for anti-MAP1B ...
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bioRxiv - Microbiology 2024Quote: ... The parasites were washed three times for 5 min in 1 x PBS before the second antibody (Alexa Fluor 647-conjugated goat anti-Rabbit antibody (Life Technologies)) ...
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bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...
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bioRxiv - Immunology 2024Quote: ... 1:500) antibodies, Alexa Fluor 546 goat anti-rabbit (#A11035, 1:500) antibodies and Hoechst 33342 (#H3570, 1:1000) from Thermo Fisher Scientific (USA) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by staining with secondary anti-Rabbit antibodies (1:500) conjugated to Alexa Fluor 555 (Goat anti-Rabbit IgG, Superclonal Recombinant Secondary Antibody, Alexa Fluor 555, Thermofisher, # A27039) for 30 minutes on a shaker at Room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... was used as a primary antibody with a goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or 633 (1:500, Thermo Scientific). The anti-Cx43 antibody targets the C-terminal domain and will therefore identify both full-length Cx43 and GJA1-20k ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Bioengineering 2023Quote: ... Primary antibodies were detected by a horseradish peroxidase (HRP)-conjugated anti-rabbit antibody using an ECL kit (Thermo Fisher Scientific, US) and imaged using ChemiDoc TM Touch Imaging System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Visualization of the primary antibody was performed using HRP-conjugated goat anti-rabbit secondary antibody (Thermo Scientific #31460; 1:10,000 dilution) or HRP-conjugated goat anti-mouse secondary antibody (Thermo scientific #31430 ...
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bioRxiv - Neuroscience 2023Quote: ... as a primary antibody and an anti-mouse IgG antibody conjugated with Alexa Fluor 488 (goat, 1/400, #A11029, Molecular Probes) as a secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... As secondary antibodies the following fluorescently labelled antibodies were used: Alexa Fluor 488 goat anti-chicken IgG (Invitrogen; A11039; 1:500) and Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection of all other primary antibodies was performed using goat anti-rabbit Poly HRP secondary antibody (Invitrogen B40962; 10 min incubation). The HRP-conjugated secondary antibody polymer was detected using fluorescent tyramide signal amplification using Opal dyes 520 ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples underwent phosphate buffered saline (PBS) washing for 15min before incubated with the second antibody HRP goat anti-rabbit antibody (ThermoFisher, 31460) which was dissolved into 1% BSA for 1h in room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed for 3X in PBS followed by secondary antibody incubation (all secondary antibodies were incubated in 1:200 dilutions: anti-mouse Fisher Scientific Cat no ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody HRP conjugated (31460) and goat anti-mouse IgG (H+L) secondary antibody HRP conjugated (31430) were from Thermo Scientific and rabbit anti-ATP13A3 antibody (HPA029471 ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated overnight with primary antibodies at 4 degrees and detected with goat anti-rabbit or mouse-HRP secondary antibodies (Thermo Scientific) followed by detection using SuperSignal™ West Pico Plus chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: ... The following primary antibodies were used: 10 ul of allophycocyanin (APC)-labeled anti-CD2 antibody (clone LT2; Invitrogen, Catalog # MA1-10132), 5 uL of APC-labeled anti-CD81 antibody (clone 1D6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated overnight with rabbit anti-p-ERM primary antibody (1:5000, Roubinet, 2011) followed by goat anti-rabbit Alexa Fluor 488-conjugated secondary antibody (1:200, Invitrogen #A11070) for 1h ...
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bioRxiv - Cancer Biology 2023Quote: ... were further incubated with fluorophore-conjugated secondary antibodies (goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 594, Thermo Scientific, cat# A-21135 ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was then incubated with a secondary antibody: goat anti-rabbit IgG (H+L) antibody (1:5000; Thermo Fisher Scientific) or Goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were left to incubated at room temperature for 90 minutes with the corresponding secondary antibodies (diluted 1:200 in PBS): Alexa Fluor® 488 Goat Anti-Rabbit IgG Antibody (Product #A-11008, Invitrogen) and Anti-Mouse IgG Secondary Antibody ...
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bioRxiv - Biochemistry 2023Quote: ... and anti-LHFPL5 antibody was labeled with Alexa647 using a Zip Alexa Fluor Rapid Antibody Labeling Kit (Thermo Fisher CAS#Z11235). Excess dye and biotin were removed using two Zeba spin desalting columns per labeling reaction and labeling was confirmed using fluorescence-detection size exclusion chromatography (FSEC ...
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bioRxiv - Microbiology 2023Quote: ... Blocking was performed for 1h (5% skimmed milk in Tris-buffered saline containing 0.5% Tween) and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... transferred to nitrocellulose membranes and the membranes were probed with the following antibodies: a rabbit anti-MAVS polyclonal antibody (Thermo Fisher), a rabbit anti-STING polyclonal antibody (Cell Signaling) ...
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bioRxiv - Molecular Biology 2023Quote: ... Incorporation of CldU was detected using rat-anti-BrdU antibodies (1:500; BU1/75, AbD Serotec) and Alexa fluor-555-labelled goat-anti-rat antibodies (1:500; Molecular Probes), whereas incorporated IdU was detected using mouse-anti-BrdU antibodies (1:750 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed and then incubated with the corresponding secondary antibodies for 1h at room temperature (Goat Anti-rabbit Secondary Antibody, HRP conjugate 1:1000, ThermoFisher #31460). All antibodies were diluted in a 5% BSA solution in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were as follows: isotype-specific Alexa Fluor 488- and Alexa Fluor 546- conjugated goat anti-rat IgG antibodies (Thermo Fisher Scientific Cat# A-21434 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 488-conjugated donkey antibody (Invitrogen A21206), (2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 647-conjugated donkey antibody (Invitrogen A31573). Washed three times for 30 minutes each in TNTT at 4 ℃ to remove secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen/ThermoFisher), and goat anti-rabbit IgG (H + L ...
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bioRxiv - Immunology 2024Quote: ... Chemiluminescence was used to detect the secondary antibody (Anti-rabbit IgG, HRP-linked Antibody #7074 and SuperSignal™ West Pico PLUS Chemiluminescent Substrate, ThermoFisher).
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bioRxiv - Biophysics 2021Quote: ... The secondary antibody Goat anti-Mouse Alexa Fluor Plus 647 (ThermoFisher scientific A32728) was used at 1/100th dilution and 300 uL of solution per chip were used ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated with rabbit monoclonal anti-vimentin antibody (SP20, Invitrogen, 1:500) in 3% BSA in PBS for one hour ...