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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Anti SUPT5H Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2, AB2251, EMD Millipore; chicken anti-GFP, A10262, Invitrogen, Carlsbad, CA) in blocking buffer (overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... The presence of relevant proteins was visualized using Alexa Fluor secondary antibodies (donkey anti-mouse AF488, anti-rabbit AF555, A-31572, Thermofisher and anti-goat AF647).
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bioRxiv - Bioengineering 2022Quote: ... Fluorophore-conjugated secondary antibody staining (anti-rabbit AlexaFluor® 488, anti-mouse AlexaFluor® 594, anti-mouse AlexaFluor® 647; Molecular Probes; 1:800) was performed at room temperature for 1 h in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 488 or 568-conjugated secondary antibodies (Donkey anti-Mouse IgG, or Donkey anti-Rabbit IgG or Donkey anti-Goat IgG; Life Technologies, 1:250 dilution) and Alexa Fluor 594 phalloidin (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated with secondary antibodies (1:500, goat anti-guinea pig Alexa488; goat anti-rabbit Alexa597; goat anti-mouse Alexa647 [Life Technologies, Eugene, OR, USA]) diluted in the same solution as primary antibody for 2 h in the dark at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated over night at 4°C with primary antibody mouse IgG2a anti-V5 tag monoclonal antibody (1/1000; Thermo Fisher Scientific Cat# R960-25 ...
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bioRxiv - Cell Biology 2020Quote: ... Plates were then incubated with anti-BrdU antibodies (G3G4, Developmental Studies Hybridoma Bank, Iowa City, IA, USA) and secondary antibodies conjugated to AF555 (A21127, Thermo Fisher), and DAPI ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked with 5% BSA in TBST buffer and probed with anti-mouse Tim-3 goat polyclonal antibody (R&D) and detected with anti-goat-HRP antibody and SuperSignal West Pico Chemiluminescent reagent (Thermo Fisher).
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bioRxiv - Developmental Biology 2020Quote: ... For detection of primary antibodies the following secondary antibodies were used: anti-rabbit IgG Alexa 488 (1:500, Invitrogen, A-11008), anti-rabbit IgG Alexa 555 (1:500 ...
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bioRxiv - Immunology 2022Quote: ... and subsequently stained for 1 h at room temperature with anti-NP mAb 1C7 antibody labeled with an Alexa488-fluorescent marker using the Alexa Fluor™ 488 Antibody Labeling Kit (Invitrogen). Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting using a rabbit polyclonal anti-syncollin antibody (Hodel and Edwardson, 2000) followed by a horseradish peroxidase-conjugated goat anti-rabbit antibody and enhanced chemiluminescence imaging (Thermo Fisher). Band intensities were quantified (in A.U. ...
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bioRxiv - Evolutionary Biology 2019Quote: ... After 45 minutes of incubation with the secondary antibody (Alexa488® Goat Anti-Rabbit IgG Antibody; Life Technologies, 1:1000 dilution) at RT ...
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bioRxiv - Microbiology 2019Quote: ... followed by an incubation with the species-specific corresponding secondary antibody (Alexa Fluor 488-, 594- or 647-conjugated goat anti mouse or rabbit antibodies, Thermo Fisher) diluted 1:2000 in 1% BSA in 1×PBS.
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bioRxiv - Genetics 2021Quote: ... or a mouse anti-HA (MBL, M180-3, 1: 200) antibody followed by incubation with fluorescently labeled secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... We then washed the brains in PTxwH at 37 °C for three days with regular buffer changes and performed secondary antibody labeling using AlexaFluor647 donkey anti-rabbit polyclonal antibody (A-31573, Thermo Fisher) at 1:1000 in PTwxH at 37 °C for 8 days ...
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bioRxiv - Neuroscience 2021Quote: ... immunogen SARS-CoV-2 spike protein that interact with HeLa cell expressed SARS-CoV-2) and anti ACE2 antibody (ACE2 Recombinant Rabbit Monoclonal Antibody (Cat# MA532307; interact with mouse ACE2) were obtained from Life Technologies Corporation.
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bioRxiv - Neuroscience 2020Quote: ... the specimens were incubated for 24 hours in secondary antibodies – goat anti-rat IgG antibodies (Alexa Fluor 488 conjugated; Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were incubated for 24 hours in secondary antibodies – goat anti-rat IgG antibodies (Alexa Fluor 488 conjugated; Molecular Probes, Invitrogen, Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... further blocking carried out with 5% milk (in PBS) for 10 minutes and incubated with secondary antibody Alexa Fluor 633 hydrazide conjugated antibody (host–goat, anti-rabbit; Life Technologies) at a dilution of (1:500 final equilibrated concentration in the dish ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of ectopic proteins was confirmed via western blotting with FHIP1B and FHIP2A-specific antibodies as well as anti-V5-HRP antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... antibody was used at 1:600 and secondary Alexa Fluor 546 anti-rabbit antibody was used at 1:500 (Molecular Probes).
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bioRxiv - Microbiology 2020Quote: ... Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Microbiology 2021Quote: ... the primary antibody was labeled with the Alexa-488 goat anti-mouse IgG secondary antibody (Invitrogen, Life Science, Eugene OR, USA) and fluorescent foci observed and counted with an Olympus BX41 microscope equipped with an UPlanFI 4× objective and a FITC filter ...
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bioRxiv - Microbiology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were blocked using 2.5% normal goat serum for 30 min then incubated in primary antibody for 1hr followed by secondary antibody mouse anti-rabbit Alexa 488 (1:1000, Molecular Probes) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary anti-mouse antibodies labeled either with Alexa Fluor® 488 secondary antibodies (green) or Alexa Fluor® 568 (Red) (Invitrogen) were used at 1:500 dilutions ...
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bioRxiv - Neuroscience 2020Quote: ... with the appropriate secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Invitrogen, A23766) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.1% Tween-20 in 1X PBS pH 7.4 and then incubated overnight at 4°C with the appropriate secondary antibodies: Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 555 (Invitrogen, A32816) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed with PBS and then incubated with secondary antibody Alexa Fluor 488-conjugated goat anti-mouse antibody (Life Technologies) diluted at 1:100 in 5% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of primary antibodies was achieved using secondary antibodies conjugated to Goat anti-mouse Alexa488 (1:400, Thermo Fisher, Cat#A28175), Goat anti-rabbit Alexa488(1:400 ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-PrimPol antibody (Table 1) was coupled to magnetic M-270 Epoxy Dynabeads® (7.5 μg antibody/ mg beads; Life Technologies), and IPs were performed following manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were visualized using donkey anti-rabbit antibody conjugated with Alexa Fluor Plus 555 (1:1000, #A32794, Thermo Fisher Scientific) and donkey anti-goat antibody conjugated with Alexa Fluor Plus 488 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 2 washes to remove any excess antibody cells were stained with anti-Rabbit Alexa-546 antibody (Life technologies, Cat#A11035). After 3 washes to remove any non-specific antibody nuclei were stained using Hoechst at 1:50,000 dilution and imaged on a Nikon Ti Eclipse automated microscope ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary anti-mouse and anti-rabbit horseradish peroxidase-conjugated antibodies and the Alexa Fluor 488- and 555-congugated antibodies were from Thermo Fisher Scientific.
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bioRxiv - Immunology 2019Quote: pOV8/Kb levels were determined by incubating cells for one h on ice with the 25-D1.16 antibody followed by one h on ice with secondary goat anti-mouse antibody conjugated to Alexa Fluor 647 (Molecular Probes). Cellular fluorescent proteins and Alexa Fluor 647 levels were determined on a FACS Calibur cytofluorograph (Beckton Dickinson ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed thrice in PBS containing 0.05% Tween 20 and incubated with secondary antibody (anti-mouse antibody conjugated to Alexa 647 or Alexa 546, Thermo Fisher Scientific) at 1:4000 dilution and GFP nano-booster (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2021Quote: ... as the primary antibody at a 1:100 dilution and anti-mouse IgG Alexa Fluor 488 as the secondary antibody (A11029, Life Technologies) at a 1:100 dilution ...
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bioRxiv - Microbiology 2020Quote: ... and rabbit anti-SERA5 (1:500) (54) primary antibodies in combination with appropriate fluorophore-coupled secondary antibodies (1:1,000; Thermo Fisher Scientific). Stainings with Lysosensor Blue DND-167 (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated with the secondary antibodies (1:1000, Goat anti-Mouse IgG secondary antibody, Alexa Fluor 488, Life technologies, USA) for 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Microbiology 2019Quote: ... and secondary antibody solution was added (1-10ug/ml) (Alexa Fluor 488 Donkey anti-mouse IgG secondary antibody, ThermoFisher, A-21202). DAPI staining was used for nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647-conjugated secondary antibodies, or anti-mouse IgG isotype-specific secondary antibodies; 1:500; Life Technologies, Carlsbad ...
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bioRxiv - Immunology 2021Quote: ... and incubated with the secondary antibody (Alexa-Fluor 647- or Alexa-Fluor 405-conjugated goat anti-mouse Ig antibodies; Invitrogen, France) for 45 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: GFP was visualized using rabbit polyclonal or mouse monoclonal anti-GFP primary antibodies and Alexa 647-labelled secondary antibodies (Molecular Probes/Invitrogen) directed against rabbit or mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Australia) primary antibody and a donkey anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor 568 (A10037; ThermoFisher, France), essentially as described (28) ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody used is goat anti-mouse IgG antibody conjugated with Alexa-568 (diluted to 2 μg/ml, A-11004, Molecular Probes).
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bioRxiv - Cancer Biology 2021Quote: ... Then coverslips were inverted onto 100 µL of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse - 1:200, Life Technologies #A11029 ...