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Citations for Thermo Fisher :
3601 - 3650 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from trophozoites of EhPTEN1 gene silenced and control strains that were cultivated in the logarithmic phase using TRIZOL reagent (Life Technologies, California, USA). Approximately one µg of DNase treated total RNA was used for cDNA synthesis using Superscript III First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... a Q-Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer in conjunction with reverse-phase HPLC-ESI-MS/MS using a Dionex UltiMate 3000 RSLC nano System (Thermo Fisher Scientific Inc.) was used ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was injected onto a reverse-phase HPLC C18 column (Biobasic-4.6mm RP-C18 5 µm, Thermo Fisher Scientific, Catalog # 2105-154630) equilibrated in 20% ACN ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were desalted on a reversed-phase PepMap 100 C18 μ-precolumn (5 μm, 100 Å, 300 μm i.d. × 5 mm, Thermo Fisher Scientific) before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were separated over a 25- cm EasySpray reversed phase LC column (75 μ m, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The mobile phases (A ...
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bioRxiv - Genetics 2020Quote: ... Resulting tryptic peptide mixtures were separated by a reversed-phase liquid chromatographic column (Acclaim PepMap RSLC column, 75 μm x 15 cm, C18, 3 μm, 100 Å, P/N164534, Thermo Fisher Scientific) to further reduce sample complexity prior to mass analyses with an LTQ Velos Pro mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... then combined into a single tube and fractionated into 12 samples using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher cat# 84868). Fractions 2 ...
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bioRxiv - Plant Biology 2023Quote: ... The metabolites were separated in the LC using a Hypersil gold C18 reversed-phase column (150 × 2.1 mm, 3µ particle size; Thermo Scientific, Waltham, Massachusetts, USA) held at 30 ᵒC ...
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bioRxiv - Systems Biology 2023Quote: ... and 5% v/v Acetonitrile (ACN) and analyzed using nano-Reverse Phase LC coupled to a QExactive Hybrid Quadrupole - Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) through a nanoelectrospray ion source (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... a 15-μg aliquot of the trypsin and GluC HeLa digest was loaded onto an Acquity UPLC M-Class CS C18 column (Waters, Etten-Leur, Netherlands) for offline reversed-phase low pH fractionation using a Dionex Ultimate 3000 HPLC system (Thermo Scientific, Bremen, Germany). Peptides were loaded using buffer A (0.1% FA in water ...
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bioRxiv - Developmental Biology 2023Quote: ... Separation was accomplished by reversed phase chromatography with an Acquity UPLC HSS T3 (Waters) on a Vanquish™ Flex Quaternary UHPLC system (Thermo Fisher Scientific). Mass spectrometry was performed on a Quantiva™ triple quadrupole mass spectrometer interfaced with an H-ESI electrospray source (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Number of cells and number of dead cells was determined on Harmony image analysis software using digital phase contrast and propidium iodide staining (Thermo Fisher Scientific, #P3566), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and analysed by LC-MS/MS using a reverse phase liquid chromatography C18 column and a triple quadrupole mass analyser (Agilent 6470 or Thermo Scientific TSQ Quantiva) instrument in positive electrospray ionisation mode ...
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bioRxiv - Plant Biology 2023Quote: ... peptide separation was performed on a reversed phase column (C18, Acclaim Pepmap C18, 75 µm x 50 cm, 2 µm particle size, 100 Å pore size, Thermo Fisher Scientific) at a flow rate of 250 nl/min using the eluents 0.1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from trophozoites of gene silenced and control strains that were cultivated in the logarithmic phase using TRIZOL reagent (Life Technologies, California, USA). Approximately 1 µg of DNase treated total RNA was used for cDNA synthesis using Superscript III First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... Digested peptides were then extracted and separated into 10 fractions by using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit according to the manufacturer’s protocol (Thermo Fisher Scientific, USA). Following fractionation ...
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bioRxiv - Bioengineering 2022Quote: ... Phase contrast microscopy (Ph) images of the cells incubated for 24 h were taken using an EVOS microscope (Thermo Fisher Scientific, Waltham, US). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: Reversed phase liquid chromatography was coupled on-line to a Q Exactive Plus Hybrid Quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a HESI probe ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was then purified before MS analysis using 25 mg HyperSep™ Hypercarb™ solid phase extraction cartridges (Thermo Fisher Scientific, Germany). The PGC material was washed with 500 μL 50% acetonitrile (0.1% formic acid) ...
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bioRxiv - Immunology 2023Quote: ... Extracted peptides were resuspended in 30μl in 1% acetic acid and injected into an Acclaim TM PepMap TM 100 Å–pore-size C18 reversed phase capillary chromatography column (Thermo Scientific TM) programmed for running in an acetonitrile/ formic acid gradient at a 0.25μl/min flow rate ...
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bioRxiv - Synthetic Biology 2024Quote: ... The organic phase samples were analyzed by gas chromatography (GC) with flame-ionization detection (FID) in a TraceTM 1300 gas chromatograph (Thermo Fisher Scientific Co.) [87–89] ...
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bioRxiv - Systems Biology 2024Quote: ... 0.1% TFA in 3.5% ACN) was directly loaded onto a reversed-phase pre-column (Acclaim PepMap 100, Thermo Fisher Scientific, Waltham, United States) and eluted in backflush mode ...
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bioRxiv - Systems Biology 2024Quote: ... Peptide separation was achieved using a reversed-phase analytical EasySpray column (Acclaim PepMap RSLC C18, 0.075mm × 250mm, Thermo Fisher Scientific, Waltham, United States) with a 120 minute linear gradient of 4%–32% solvent B (0.1% TFA in 80% ACN ...
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bioRxiv - Biochemistry 2023Quote: ... The pooled TMT-labeled peptide sample was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific 84868). We followed the manufacturer’s instructions except that the percentage of acetonitrile used in the elution buffers and the subsequent concatenation strategy was performed as described below ...
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bioRxiv - Molecular Biology 2023Quote: ... and two solid-phase extraction columns (150 µm × 3 cm) packed with MAbPac particles (C8 resin, 4 µm diameter, 300-Å pore size; Thermo Fisher Scientific) were used ...
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bioRxiv - Plant Biology 2023Quote: ... The peptides were separated on a reversed-phase Easy-spray PepMap nanocolumn (75 μM inner diameter × 50 cm, 2 μM particle size, 100 Å pore size; Thermo Fisher Scientific) with a 4–76% acetonitrile gradient in 0.1% formic acid (total time ...
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bioRxiv - Systems Biology 2023Quote: ... cells reached the logarithmic growth phase and were detached from the plate surface using 0.25% trypsin–EDTA (Thermo Fisher Scientific, Waltham, MA, US). The cell viability was evaluated using the Trypan Blue Assay (Strober ...
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bioRxiv - Molecular Biology 2022Quote: Size based RNA separation using ion-pair reverse phase (IP-RP) chromatography was performed on an Thermo Vanquish UHPLC instrument (ThermoFisher Scientific, Waltham, MA). The chromatography was carried out under denaturing conditions at 65°C ...
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bioRxiv - Microbiology 2022Quote: ... NulO derivatives were separated isocratically on a C18 reverse-phase HPLC column (Thermo Scientific, Hypersil ODS, 4.6 mm by 250 mm, 5 µm) using acetonitrile/methanol/water (7:9:84 vol/vol/vol ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were first trapped for 3 min on a C18-reverse phase trapping column (Acclaim PepMap® 100, 75 μm x 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), followed by separation on a C18-reverse phase analytical column (Acclaim PepMap® 100 ...
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bioRxiv - Microbiology 2022Quote: ... followed by separation on a C18-reverse phase analytical column (Acclaim PepMap® 100, 75 μm x 25 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific) with a solvent flowrate of 300 nL/min and a column temperature of 35°C ...
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bioRxiv - Neuroscience 2022Quote: ... The mixed SARM/PLGA solution was added to an aqueous phase (30 mL; 1% w/v polyvinyl alcohol in Nanopure water; Barnstead Thermolyne Nanopure water purification system, Thermo Fisher,Waltham, MA) and the mixture was immediately emulsified using either a Talboys Model 101 overhead mixer at speed 2833 rpm for 4 minutes (formulation 1 ...
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bioRxiv - Cell Biology 2023Quote: The peptides were subjected to a reversed-phase Nano LC-MS/MS (EASY-nLC, Thermo) connected to a Q Exactive quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen). The peptides in the fractions were directly loaded onto an in-house packed 100 μm i.d ...
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bioRxiv - Synthetic Biology 2023Quote: Total yeast RNA isolation was performed on OD ∼ 1 of yeast cells collected from mid-log phase growth using the GeneJet RNA Purification Kit (Thermofisher Scientific Catalog #: K0731) according to the instructions for isolating yeast RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6 μm particle size ...
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bioRxiv - Biochemistry 2023Quote: Reversed phase liquid chromatography was coupled on-line to a Q Exactive Plus Hybrid Quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a HESI probe ...
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bioRxiv - Bioengineering 2023Quote: Cells grown to mid-log phase were fluorescently labeled using 20 μM SYTO® BC Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific) in their growth medium ...
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bioRxiv - Physiology 2023Quote: ... The organic phase of each sample was normalized by total soluble protein amounts and measured by BCA assay (Thermo Scientific, 23225, Waltham, MA). After vortexing ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 2 μL injections were analyzed by reversed phase nano-liquid chromatography - mass spectrometry (nano-LC-MS/MS) using an Orbitrap Exploris 480 Mass Spectrometer (Thermo Scientific, Bremen, Germany) interfaced with an EASY nanoLC-1200 system (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... separated over a 50 cm EasySpray reversed phase LC column (75 µm inner diameter packed with 2 μm, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The solvents (A ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was prepared from the stationary-phase cultures of the new isolates using PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, USA). The 16S rRNA gene of each strain was amplified using PCR ...
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... wherein peptides were separated over a 25 cm EasySpray reversed phase LC column (75 µm inner diameter packed with 2 μm, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The mobile phases (A ...
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bioRxiv - Molecular Biology 2024Quote: 5 mg DNA of KBTBD4 alone or CoREST1NT2 and HDAC2 constructs at 1:1 ratio was transfected into 1L Expi293FTM cell culture in mid-log phase (2LJ×LJ106 mL-1) in Freestyle 293TM Expression Medium (Thermo Fisher Scientific #12338018) with 15 mg PEI-MAX 40 kDA ...
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bioRxiv - Biochemistry 2024Quote: Reversed phase liquid chromatography was coupled on-line to a Q-Exactive Plus Hybrid Quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a HESI probe ...
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bioRxiv - Microbiology 2024Quote: ... These were incubated until the cultures reached an optical density at 600nm (OD600) of approximately 0.8 (mid- to late-log phase) measured with a Genesys 30 Visual Spectrophotometer (Thermo Scientific, Waltham, MA, USA). Tubes for culturing biofilms under batch and static conditions were prepared by placing a halved borosilicate glass slide (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were desalted on a reverse-phase PepMap 100 C18 μ-precolumn (5 mm, 100 Å, 300 mm i.d. × 5 mm, Thermo Scientific™) and separated on a 50-cm EasySpray column (75 mm ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides obtained from soluble or membrane protein fractions were purified by C18 solid phase extraction using the C18 Spin column (Pierce Biotechnology, Thermo Scientific, Rockford, IL) and per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Peptides were separated by reversed-phase capillary HPLC on an UltiMate 3000 nano system (Dionex) coupled in-line to an Orbitrap Fusion mass spectrometer (Thermo Scientific, Bremen, Germany). Peptides were loaded onto the trapping column (PepMap100 ...