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Citations for Thermo Fisher :
3551 - 3600 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... pBSDONR(P1-P4) (Qi et al. 2012) using BP Clonase II (Invitrogen) and the resulting construct was designated pBSDONR(P1-P4):FgTPP1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Viability and cell count were assessed on Countess II (Thermo Fisher Scientific). Suspensions were equilibrated to targeted cell recovery of 10,000 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... [30] using the Gateway® LR Clonase® II Enzyme Mix (Invitrogen). The pDONR223-PLK1 plasmid (Addgene plasmid #23867 ...
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bioRxiv - Genomics 2024Quote: ... Intact cells were counted using a Countess II (Thermo Fisher, Waltham, MA) and cell viability was confirmed to be >65% ...
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bioRxiv - Genetics 2024Quote: ... RNA samples were combined with 2X Gel Loading Buffer II (Thermofisher AM8546G) and denatured at 95°C for 5 minutes ...
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bioRxiv - Genomics 2024Quote: ... Three technical replicates of 1 unit of SuperScript II (Invitrogen; ref. 18064022) were used for relative quantification of RT activity ...
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bioRxiv - Systems Biology 2024Quote: ... The fragments were then recombined into pDONR221 using BP II clonase (Invitrogen) and sequence determined by sanger sequencing (Genepool ...
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bioRxiv - Genetics 2024Quote: Preparation of cDNA via reverse transcription was performed using Superscript II (Invitrogen) using an oligo-dT17V primer as previously described (Haussmann et al ...
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bioRxiv - Systems Biology 2024Quote: ... and reverse transcribed into cDNA using the Superscript-II kit (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... After counting the number of cells using Countess II (Thermo Fisher Scientific), the cell suspension was diluted to 10-30 cells/μL ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse transcribed with Superscript II reverse transcriptase (Invitrogen, Carlsbad CA, USA) and random hexamers (Promega) ...
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bioRxiv - Biophysics 2024Quote: ... to produce lentiviral expression vectors using Gateway LR Clonase II (Invitrogen, #11791020). The resulting plasmids can be used either for transient transfection or as transfer plasmids for lentivirus production.
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bioRxiv - Cell Biology 2024Quote: ... Cells were counted using Countess II FL (Thermo Fisher, Waltham, MA, USA), centrifuged for 10 min at 500 x g and 4°C ...
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Scalable Fabrication of a Tough and Recyclable Spore-Bearing Biocomposite Thermoplastic PolyurethanebioRxiv - Bioengineering 2024Quote: ... The filament was introduced into HAAKETM MiniLab II extruder (Thermo-Fisher Scientific) at 145 °C at 36 rpm under continuous mode ...
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bioRxiv - Bioengineering 2024Quote: ... with 5% PFHM-II protein-free hybridoma medium (12040077, Thermo Fisher Scientific), 100 units/mL penicillin ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell number was counted with Countess II Cell Counter (Thermo Fisher Scientific). A total of 50,000 cells (12,500 cells per sample ...
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bioRxiv - Cancer Biology 2024Quote: ... One million cells were counted using the cell counter Countess II (Invitrogen) and pelleted for protein extraction with Cell Lysis Buffer (Cell Signaling Technology Catalog No ...
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bioRxiv - Cancer Biology 2024Quote: ... JAWS II (obtained from ATCC) cells were cultured in IMDM (Gibco, 12440053) with 10% FBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used an automated cell counter (Cell Countess II FL, Thermo Fisher) to quantify the total number of spores ...
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bioRxiv - Genetics 2024Quote: ... 10 μL Platinum II hot-start Green master mix (Invitrogen, cat 14001012), and 0.5 μL each of forward and reverse primers (10 μM stocks) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cell viability was assessed on a Countess II automated cell counter (ThermoFisher), and an estimated 12,000 cells were loaded onto one lane of a 10x Genomics Chromium Controller ...
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bioRxiv - Cell Biology 2024Quote: ... anesthetized fish were moved to 1-well (Nunc Lab-Tek II 155360) glass-bottom imaging chambers with a transfer pipet (Fisher 13-711-7M ...
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bioRxiv - Developmental Biology 2024Quote: ... SuperScript II Reverse Transcriptase was used for second strand synthesis (Invitrogen #100004925). cDNA was amplified with 14 PCR cycles ...
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bioRxiv - Developmental Biology 2024Quote: ... Fragments were cloned into the pCR-Blunt II-TOPO vector (Life Technologies) and sequence-verified prior to plasmid linearization with EcoRV and BamHI ...
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bioRxiv - Cancer Biology 2021Quote: ... Tryptic (glyco)peptide mixtures were separated over a 25 cm EasySpray reversed phase LC column (75 μm inner diameter packed with 2 μm, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The mobile phases (A ...
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bioRxiv - Cell Biology 2020Quote: Each TMT labeled peptide set was fractionated to improve depth of proteome coverage using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific, Part 84868). The fractionation was performed according to the manufacturer’s protocol and yielded 8 peptide fractions for analysis ...
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bioRxiv - Genetics 2020Quote: ... 35 µg were subjected to fractionation by high-pH reversed phase liquid chromatography (RPLC) using an Ultimate 3000 HPLC (high performance liquid chromatography) (Thermo Scientific, Dreieich, Germany) equipped with a C18 column (BioBasic 18 ...
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bioRxiv - Cancer Biology 2020Quote: ... and a heated (50 °C) reversed-phase analytical column (Acclaim PepMap RSLC C18, 2 μm, 100 Å, 75 μm × 500 mm, nanoViper, ThermoFisher Scientific, Inc) connected online with an Orbitrap mass spectrometer ...
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bioRxiv - Molecular Biology 2021Quote: Twenty microliters of tryptic digested peptides were injected on a reversed phase trap column (Analytical Column LC18 BioBasicTM, 300 Å, 5 µm, 50 µm ID × 1 mm length, Thermo Scientific, US). Separations were performed using an ultra-chromatographic system (UltiMate 3000 RSLC System ...
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bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: The qualitative viability of spheroids formed in 600 μm micromolds or in micromolds of the periapical lesion and at the exponential growth phase was evaluated using the Live/Dead™ Viability/Cytotoxicity Kit (Invitrogen-Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded onto a reverse phase trap column (Acclaim PepMap 100, 75µm x 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, U.S.A.) using an Ultimate 3000 for 50 µL at a flow rate of 10 µL min-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Both analytes were separated on a Hypersil™ BDS C18 column (150 mm, VDS-optilab) using isocratic flow of the mobile phase MD-TM (Thermo Fisher Scientific) at a rate of 0.5 ml/min ...
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bioRxiv - Microbiology 2020Quote: ... The separation was performed for 60 min at the flow rate of 300 nL/min using C18-reverse phase column (EASY-Spray™, Thermo Fisher Scientific). The stepwise gradient was set from 5% solvent B (0.1% formic acid in acetonitrile)/ 95% solvent A (0.1% formic acid in H2O ...
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bioRxiv - Systems Biology 2021Quote: ... The separation of glycan was performed on an analytical column packed with PGC phase beads (Hypercarb 3 µm; pore size 250 Å; Thermo Fisher Scientific) with 30 min from 5 to 35% acetonitrile (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... The desalted peptides were then separated using a 15 cm pre-packed reverse phase analytical column (Acclaim PepMap 100, 75μm x 15 cm, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using a 45 min linear gradient from 5% to 40% solvent C (80% acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were separated over a 50 cm EasySpray reversed phase LC column (75 µm inner diameter packed with 2 μm, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The mobile phases (A ...
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bioRxiv - Cancer Biology 2020Quote: ... dried at 30°C in a vacuum concentrator and fractionated using a Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded on a reverse phase EASY-Spray− column (50 cm × 75 m inner diameter) operated using Easy-nLC− 1200 μ (Thermo Fisher Scientific) coupled online to a Q-Exactive HF-X mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... the trap column was switched in-line with an Acclaim PepMap100 reversed phase column (75 µm x 50 cm, 2 µm particle sizes, 100 Å pore size; Thermo Fisher Scientific) for peptide separation ...
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bioRxiv - Biochemistry 2022Quote: ... into a reversed phase trap column (100μm ID x 50 mm, packed with Poros R2 10 μm C18 particles (Life Technologies, Norwalk, CT, USA) using a vented trap-elute configuration ...
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bioRxiv - Immunology 2022Quote: ... Samples were vortexed and spun at 12000 x g for 5 minutes to achieve phase separation followed by addition of 12 μL glycogen (Thermo Fisher, Cat# 10814010). The DNA-containing upper aqueous phase was transferred to a fresh tube and placed on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Quantification of CoQ9 and CoQ10 was determined using Agilent 1100 HPLC system on a C18-ODS Hypersil reverse phase column (Thermo Scientific #30105-254630). Samples were spiked with CoQ4 to calculate the extraction and recovery efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... For separation a reversed-phase Acclaim PepMap RSLC column (150 mm × 75 μm, particle size 2 μm, pore size 100 Å, Thermo Fisher Scientific) was used ...
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bioRxiv - Microbiology 2020Quote: ... The total lipid extract was analyzed on an Agilent 1200 reversed phase high performance liquid chromatograph (HPLC) coupled to a ThermoFisher Exactive Plus Orbitrap high resolution mass spectrometer (HRMS; ThermoFisher, Waltham, MA, USA). HPLC and MS conditions are described in Collins et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA extraction: RNA was precipitated by mixing the samples of the aqueous phase with 0.5 ml isopropanol (Fisher scientific, Cat. No. A416P-4), incubation at RT for 15 min and spin at 12,000 x g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... and a heated (50 °C) reversed-phase analytical column (Acclaim PepMap RSLC C18, 2 µm, 100 Å, 75 µm × 500 mm, nanoViper, Thermo Fisher Scientific) coupled online with the MS ...
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bioRxiv - Microbiology 2020Quote: ... The peptide samples were loaded (2 µl) onto a C18-reversed phase Acclaim PepMap 100 trap column (2 cm x 75 µm, 3 µm particles; Thermo Fisher Scientific, USA) with 30 µL of buffer A (2% v/v acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: Cells from one-day-old culture - at the mid-exponential growth phase - at an initial cell density of 1×105 cells / ml were seeded in 12-well plates (Nunc/DD Biolab, #55428) with 1 ml marine broth and incubated at 23 ºC ...
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bioRxiv - Plant Biology 2020Quote: ... to be further separated on a 50 cm C18 reversed phase analytical column (Acclaim PepMap100, diameter: 75 μm, granulometry: 3 μm, pore size: 100 Å; Thermo Fisher Scientific). Peptides were eluted by a non-linear 5-36% [v/v] acetonitrile gradient in 0.1% [v/v] formic acid at a flow rate of 250 nl min-1 over a period of 60 min at 33° C ...