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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The amplification and fluorescence detection of quantitative RT-qPCR was performed in Q6 (Invitrogen), using the TAKARA TB Green Premix (RR420) ...
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bioRxiv - Genetics 2023Quote: ... Signal detection was achieved using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system).29,30 Target gene expression was normalized to a housekeeping gene (ribosomal protein S18 ...
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bioRxiv - Genomics 2023Quote: ... Chromatographic separation and detection were performed on a Vanquish Flex system (Thermo Fisher Scientific) coupled with a TSQ Quantiva triple quadrupole mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was achieved using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, #34580).
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bioRxiv - Bioengineering 2023Quote: ... and samples were amplified on an ABI Prism 7900HT Fast Detection System (Applied Biosystems). Gene expression was normalized against GAPDH endogenous controls.
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bioRxiv - Microbiology 2023Quote: ... The data were analyzed using ABI PRISM 7500 Sequence Detection System Software (Applied Biosystems). The primers are listed in Table S4.
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bioRxiv - Cancer Biology 2023Quote: ... the blots were developed using enhanced chemiluminescence western blotting detection reagent (Thermo Fisher Scientific). Signals were captured using CCD camera of the ChemiDoc XRS+ system (BioRad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... The ABI Prism 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA) was used for qPCR analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoblot signals were detected using an ECL detection reagent (Thermo Fisher Scientific, 34577, 34095).
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bioRxiv - Cell Biology 2023Quote: ... or for more sensitive detection SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific. All uncropped blots are shown in Figures S8 and S9 ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptotic cells were detected with CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (1:500) ...
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bioRxiv - Microbiology 2023Quote: ... The detection system (Q-Exactive Focus/Ultimate 3000; Thermo Fisher Scientific, Waltham, MA, USA) was equipped with a 100 x 2.1 mm ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by secondary antibody incubation and detection with ECL chemiluminescence reagent (Invitrogen). Protein expression levels were represented as fold change relative to actin.
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bioRxiv - Cell Biology 2024Quote: ... was used for RT-PCR amplification and detection was performed using ViiA7 (Life Technologies) or Step One Plus Real-Time PCR system(Applied Biolsystems) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by detection using the ECL Prime Western Blotting System (Thermo Fisher Scientific 32,106).
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bioRxiv - Neuroscience 2024Quote: ... The washed membranes were then treated with an enhanced chemiluminescence detection reagent (Thermo Scientific). All Blots were developed using ChemiDoc XRS+ Molecular Imager (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... AlexaFluor-conjugated secondary antibodies raised in donkey were used for signal detection (Thermo Fisher Scientific #A31571 ...
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bioRxiv - Microbiology 2024Quote: ... Detection is made using the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher #34580). Acquisitions were made by exposition of autoradiography films (USA Scientific #19683810) ...
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bioRxiv - Immunology 2021Quote: ... ELISAs were performed using Nunc MaxiSorp™ flat-bottom 96-well plates and the respective Invitrogen™ Uncoated ELISA Kits (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were sorted in a BD FACSAria™ III into 96-well plates loaded with RT-STA reaction mix (CellsDirect™ One-Step qRTPCR Kit, Invitrogen) and 0.2x specific TaqMan ® Assay mix (see Table 1 for assays list).
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bioRxiv - Neuroscience 2019Quote: ... that were either positive or negative for NIAD4 were sorted into 96 well plates (10 cells/well) containing 10 μl of Lysis Buffer from the Single Cell to Ct kit (Thermo Fisher Scientific), using the FACSAria™ III cell sorter ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were sorted in a BD FACSAria™ III directly into 96-well plates loaded with RT-STA Reaction mix (CellsDirect™ One-Step qRTPCR Kit, Invitrogen, according to the manufacturer’s procedures ...
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bioRxiv - Bioengineering 2021Quote: ... The 300 μl collected was aliquoted into an individual well in a 96-well microplate containing 100 μl each (3 wells total for a single well in the 24-well plate) and subjected to a CyQuant© Direct Proliferation Assay Kit (Thermo Fisher) per the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... aliquots of SAGs were transferred to replica plates for DNA yield quantification using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific). For sequencing analysis ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed in 96 well plates and RNA was extracted using Applied Biosystems MagMax 96 total RNA isolation kit (Thermo Fisher, # AM1830) with on bead DNase digestion ...
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bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
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bioRxiv - Immunology 2020Quote: ... Each plate was pooled and the plate libraries were quantified on a Qubit Fluorometer 3.0 using a dsDNA High-sensitivity Kit (Life Technologies, Carlsbad, CA). The plates were then pooled in equal volumes and the pooled library was then diluted to 6 μM and sequenced using a 500-cycle V3 reagent on an Illumina MiSeq (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sizes were determined using an Agilent 2200 TapeStation system (∼550bp) and libraries normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific). Library concentration was calculated using a SYBR green quantitative PCR (qPCR ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were performed on a 100-μl scale in a 96-well plate according to the “Amplex™ Red Cholesterol Assay Kit” protocol (ThermoFisher Scientific). No cholesterol esterase was added ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted in a 96-well format from the consolidated Prospector® culture plates using the Extract All Kit (Applied Biosystems). 20µL of culture was combined with 20µL of Lysis Solution and incubated for 10 minutes at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of SAGs were transferred to replica plates for DNA yield quantification using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Thermo Fisher Scientific) prior to library preparation ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
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bioRxiv - Molecular Biology 2020Quote: Total cell lysate was prepared by adding Laemmli sample buffer 1X supplemented with Pierce™ Universal Nuclease (Thermo Scientific™), phosphatase inhibitor cocktail and protease inhibitor cocktail directly to the cell monolayer ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently measured by real-time PCR with the Taqman universal PCR master mix and specific Taqman probes (Applied Biosystems). Sequences of primers are shown in Supplementary Table 2.
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bioRxiv - Genetics 2019Quote: ... The expression of selected genes was measured using iTaq Universal SYBR Green Supermix (company) and the ViiA detector (Applied Biosystems). Each biological replicate was run in triplicate ...
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bioRxiv - Genomics 2019Quote: ... Quantitative real time PCR reactions were performed with the ABI 7900 Real Time PCR System using Universal PCR Master Mix (Thermofisher), with initial activation at 50°C for 120 seconds and 95°C for 10 minutes ...
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bioRxiv - Physiology 2020Quote: ... 1 μl FAM™ probe and 10 μl TaqMan Universal Master Mix (Applied biosystems by Thermo Fisher Scientific, Epsom, UK) was amplified as follows ...
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bioRxiv - Physiology 2020Quote: ... 1 μl FAM™ probe and 10 μl TaqMan Universal Master Mix (Applied biosystems by Thermo Fisher Scientific, Epsom, UK) was amplified as follows ...
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bioRxiv - Developmental Biology 2019Quote: ... and mir152 specific primers with NCode miRNA First-Strand cDNA Synthesis qPCR Universal Primer in a fast mode SybrGreen reaction at the QuantStudio 5 (Thermofisher). We used RNU6B and RNU44 as endogenous controls ...
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bioRxiv - Plant Biology 2019Quote: ... Every qRT-PCR reaction was carried out in a 10μL volume containing: 5 μL SsoAdvanced Universal SYBR Green Supermix (Applied Biosystems), 1 uL template ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted 1:5 in TE buffer and 6% were mixed with TaqMan Universal PCR Master Mix (Applied Biosystems). The sample mix and TaqMan assays were loaded separately into the wells of 96.96 Gene expression Dynamic Arrays (Fluidigm ...
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bioRxiv - Microbiology 2021Quote: Approximately 200 ng of extracted vRNA was reverse transcribed using a universal 3′ primer (5′-AGGGCTCTTCGGCCAGCRAAAGCAGG) and Superscript III reverse transcriptase (RT) (Invitrogen). The RT product was diluted approximately 10,000-fold and used as a template for quantitative PCR (qPCR) ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was reverse-transcribed with 0.5ug of poly (T) adaptor containing a 5’-end universal tag sequence and SuperScript™ II Reverse Transcriptase (Thermofisher). Specific miRNA primers ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was run in triplicate in 20 µl of reaction volume using TaqMan Universal PCR Master Mix according to the manufacturer’s instructions (ThermoFisher Scientific). All reactions were performed in a 7500 Real Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... real-time quantitative PCR was performed using TaqMan Fast Universal PCR Master Mix (4366072) and TaqMan probes (Vcam1, Mm01320970_m1; Gapdh, Mm99999915_g1; both Thermo Fisher Scientific). Reactions were performed in a total volume of 10 μl on a ViiA 7 system (Thermo Fisher Scientific) ...