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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by BCA Protein Assays (Thermo Scientific) 30 μg of total lysate were separated by 4–12% NuPage Bis-Tris PAGE (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... protein concentration was measured by Pierce 660nm Protein Assay (ThermoFisher). The volume corresponding to 100 μg protein was transferred to a 96-well 2 ml deep-well plate (Eppendorf ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR assays were performed using TaqMan Gene Expression Master Mix and the probes human NEMO/IKBKG (Hs00415849_m1) and human GAPDH (Hs03929097_g1) (ThermoFisher Scientific). Amplifications were run in a Bio-Rad CFX96 real-time PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... The second incubation were performed with secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch; Anti-Human IgM-Cy5, Invitrogen) diluted in ratio 1:1000 in incubation buffer with 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: Human monocytic U937 cells (ATCC) and human embryonic kidney 293T cells (ATCC) were cultured in RPMI 1640 medium (Gibco), supplemented with 5-10% fetal calf serum (BioWest) ...
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bioRxiv - Cancer Biology 2021Quote: Human HCC cell lines (Huh7, HepG2) and Human HeLa were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
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bioRxiv - Molecular Biology 2023Quote: ... Hep-3B human hepatocellular carcinoma cells and MCF7 human mammary carcinoma cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Gibco) supplemented with 10% heat-inactivated and charcoal stripped fetal calf serum and antibiotics ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Both Fn39-10 S1417C mutant and its inactive version were biotinylated with Maleimide-PEG11-Biotin at residue 1417 (ThermoFisher Scientific) in PBS.
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bioRxiv - Developmental Biology 2020Quote: ... and biotinylated EMSA probes and unlabeled competitor probes were synthesized by using a LightShift® Chemiluminescent EMSA Kit (Thermo Scientific). The daf-7p-WT-CRE and daf-7p-mut-CRE probes with the following sequences were employed:
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bioRxiv - Immunology 2022Quote: ... CCL2 was affinity purified from the in vitro reactions using Phytip streptavidin coated tips (Phynexus) bound to biotinylated anti-CCL2 antibody (Invitrogen) using the Phytip protocol ...
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bioRxiv - Immunology 2022Quote: All antigens were biotinylated using an NHS-Sulfo-LC-LC kit according to the manufacturer’s instruction (Thermo Fisher, MA, USA) if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff ...
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bioRxiv - Genomics 2020Quote: ... Biotinylation efficiency was assessed by running an aliquot of the biotinylated oligo alongside unbiotinylated oligo of the same sequence and 10 bp DNA ladder (ThermoFisher) on a 20% TBE-Urea PAGE gel (SequaGel ...
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bioRxiv - Microbiology 2021Quote: ... The biotinylated cDNA probe for each segment was mixed with the Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific, Waltham, MA) and incubated on a rotating wheel at 37°C for 1 h in a LiCl hybridization buffer (10 mM Tris-HCl [pH 7.9] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hybridization and detection of biotinylated probes was performed using the North2South™ Chemiluminescent Hybridization and Detection Kit (Thermo Fisher Scientific) following manufacturer instructions.
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bioRxiv - Bioengineering 2019Quote: ... These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific). Excessive biotin was removed by buffer-exchange into PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The mRNA concentrations and integrity were determined with Agilent’s NanoDrop instrument then amplified and biotinylated with the Affymetrix GeneChip 3’ IVT PLUS Reagent kit (ThermoFisher Scientific) following manufactirer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... and the biotinylated nascent transcripts were isolated in a total of three rounds of streptavidin-coated magnetic bead (M-280, Invitrogen) purifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated cDNAs were prepared from a minimal 100ng of total RNA using Life Technologies WT-plus RNA Amplification system (Ambion). Following the amplification ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo scientific) or fluorescent secondary antibody to visualize proteins ...
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bioRxiv - Genomics 2020Quote: ... The DNA was eluted in 150 μL 10 mM Tris-HCl pH 8 and the biotinylated molecules captured with Dynabeads M280 (Invitrogen) according to the manufacturer’s protocol but using150 μL Bead Binding buffer (Phase Genomics ...
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bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and HAΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated with a 20-fold excess of 10 mM EZ-Link NHS-LC-biotin (ThermoFisher) and unbound biotin was removed using a Zeba Spin Desalting Column (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked and hybridized overnight with 100 ng/mL of 5′ biotinylated DNA probe (Table S3) in ULTRAhyb (Ambion) hybridization buffer at 42°C ...
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bioRxiv - Microbiology 2020Quote: ... TUNEL-labeled DNA was detected using a biotinylated anti-BrdU antibody and streptavidin-Alexa Fluor 594 conjugate (ThermoFisher Scientific, Australia). At least 100 infected cells were counted on each slide ...
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bioRxiv - Cell Biology 2021Quote: ... a 3-step approach was taken using a biotinylated secondary antibody and a final incubation step with a strepatividin-linked AlexaFluor antibody (Invitrogen). Slides were inserted into a 1 μg/ml solution of 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2020Quote: ... sodium azide and endotoxin were removed from the biotinylated antibodies with Zeba desalting spin columns and High-Capacity Endotoxin Removal spin columns (ThermoFisher) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Biotinylated SARS-CoV-2 prefusion stabilized S trimer was incubated with yellow-green streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo Scientific) or fluorescent secondary antibodies with species-specific Alexa Fluor-488 or -568 (Invitrogen A-11039 or A-11011 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were purified by phenol/chloroform extraction and RNA precipitation (total RNA) or MyOne Streptavidin C1 beads (biotinylated probes; Invitrogen). For total RNA purification ...
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bioRxiv - Developmental Biology 2022Quote: Biotinylated bait ectodomain transfections were dialysed against PBS using SnakeSkin dialysis tubing (molecular weight cut-off 10,000 Da; Thermo Scientific) and several buffer changes (approximately 25 – 30 L in total) ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were hybridized at 42 °C with 5 nM of a 5’-biotinylated oligonucleotide probe (Table S4) in ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) and then washed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Transferred RNA was UV cross-linked and hybridized overnight with 100 ng/mL of 5’-biotinylated probes (Table S2) in ULTRAhyb hybridization buffer (Ambion) at 42 °C as described previously (27 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were incubated with biotinylated RBD, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidinphycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at ¼ concentration of the biotinylated RBD ...
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bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μl pre-mix (25 μl 0.4 mM Biotin-dATP (Invitrogen, #19524016), 25 μl 0.4 mM Biotin-dCTP (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Amplified and biotinylated sense-strand DNA targets were generated from total RNA using the WT Plus Kit (Thermo Fisher, MA). Fragmented and biotinylated sense-strand DNA target (5.5 µg ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... 300 μg of purified monoclonal antibodies were chemically biotinylated using a 20-fold molar excess of sulfo-NHS-biotin (ThermoFisher) for two hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2019Quote: ... mucins were also detected using biotinylated Ulex europaeus agglutinin I (UEA-I) lectin to detect fucose residues (Vector, Burlingame, CA) or with biotinylated maleimide to alkylate and detect reduced sulfhydryls (ThermoFisher).