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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: T7 double stranded DNAs (T7-dsDNAs) were amplified by T7-tagged primer pairs (Table 2) by Phire Hot Start II PCR Master Mix kit (Thermo Fisher, Cat # F125L) using proper plasmids as templates ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with 0.1% PBS-TX three times and treated with Alexa Fluor 488-tagged goat-anti-rabbit IgG antibodies (Thermo Fisher, Cat # A-11008), diluted 1:800 in 0.1% PBS-TX for 6–12 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: HEK293T or HeLa Kyoto cells expressing the indicated tagged proteins were washed twice with ice-cold PBS and lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) containing protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... Human kidney cells (HKC) and human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. # 11965126). Mouse embryonic fibroblasts (MEF ...
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bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
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bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
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bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
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bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... The genomic titers of DNase-resistant recombinant AAV particles were determined after alkaline treatment of virus particles and subsequent neutralization by qPCR using the qPCRBIO SY Green Mix Hi-Rox (Nippon Genetics Europe GmbH) and an ABI PRISM 7900HT cycler (Applied Biosystems). Vectors were quantified using primers specific for the GCaMP6s sequence (5’-CACAGAAGCAGAGCTGCAG and 5’-actggggaggggtcacag) ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA fragment encoding the full-length Npbwr2 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The resulting Npbwr2 expression construct was transiently transfected into HEK293T cells together with the cAMP-responsive luciferase reporter vector pGL4.29 (Promega ...
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bioRxiv - Pathology 2019Quote: ... The pooled PCR products were added to 9.9 μL of Hi-Di formamide and 0.1 μL of GeneScan™ 500 LIZ™ size standard (Life Technologies). Samples were denatured at 94 C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR and Ion Sphere Particles (ISP) enrichment were done using the Ion PGM Hi-Q Kit for 200bp (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg of His-SUMO-SecA purified from untreated cells was bound to 100 μl Ni-NTA agarose beads (Life Technologies). The beads were incubated with 500 μl of lysis buffer or buffer containing 2 mM sodium azide for 10 minutes and washed three times with 500 μl lysis buffer ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... from a cDNA library and cloned into pcDNA™3.1/His expression vectors (Catalog #V38520, Life Technologies Co., Grand Island, NY). Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170 ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing products were purified using 75% isopropanol and resuspended in 12 μl of Hi-Di™ Formamide (Thermo Fisher Scientific). After loading on the Applied Biosystems® 3130 Genetic Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The cells after incubation were washed with FACS buffer and incubated with anti-his Alexa Fluor 647 antibody (Thermo Fisher Scientific) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: 500 ng to 2000 ng of Hi-C library DNA or genomic DNA library were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 μl dH2O ...
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bioRxiv - Microbiology 2020Quote: ... We then resuspended the cell pellet in 9.5mL PBS/2%HI-FBS for counting using the Countess Cell Counting system (Thermo Fisher Scientific). We aliquoted 0.5 mL for Seq-Well ...
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bioRxiv - Microbiology 2021Quote: ... Serum supernatant was collected as described above and incubated with a pellet of 900 μl Dynabeads™ His-Tag Isolation & Pulldown (Invitrogen) equilibrated in wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... experiments were performed in total reaction volume of 10μl with qPCRBIO SyGreen Blue Mix Hi-ROX (PCR BIOSYSTEMS) reagent on 384-wells plates (Axygen) using Viia7 (Thermo Fisher Scientific) instrument in standard conditions.
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2020Quote: ... samples were sequenced on an Ion PGM Sequencer with Ion PGM Hi-Q View Sequence Solutions (Ion Torrent Life Technologies, USA).
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the serum was collected then affinity purified using His-CSAG1 linked to the amino link plus coupling resins (Thermo scientific, #20501) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Emulsion PCR and Enrichment was performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (Thermofisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; Thermofisher Scientific). The Ion Proton reads (FASTQ files ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... Emulsion PCR and enrichment of template-positive particles were performed using an Ion PGM Hi-Q OT2 Kit (Thermo Fisher Scientific) and the Ion OneTouch 2 system (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; A26433, Thermofisher Scientific). Sequencing reads were trimmed with Prinseq (160 ...
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bioRxiv - Developmental Biology 2020Quote: ... Emulsion PCR and enrichment was performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (A26434, Thermofisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length ...