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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
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bioRxiv - Cell Biology 2023Quote: HCT116 human colorectal cancer and SUIT-2 human pancreatic ductal adenocarcinoma cells were grown in McCoy’s 5A medium (Gibco, Cat. # 26600080) supplemented with 10% v/v dialyzed Foetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell binding experiment was done with the human T lymphocyte cell Jurkat and anti-human IgG antibody-Ax488 (ThermoFisher Scientific). Ax488 signal changes upon binding were detected using a flow cytometer (BD Accuri C6 ...
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bioRxiv - Immunology 2023Quote: ... Human serum used for all conditions was from the same lot of pooled human AB serum (Fisher Scientific, Cat#: BP2525-100). For heat inactivation ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Cell Biology 2024Quote: HepG2 (Human hepatoma cell line, ATCC, Manassas, Virginia, United States of America) and HEK293A (Human embryonic kidney cell line, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel droplets were overlaid with recombinant organoid medium: Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed as a soluble recombinant protein in mammalian Expi293 cells (Thermo Fisher Scientific). The secreted protein self -assembles into a protein nanoparticle ...
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bioRxiv - Microbiology 2022Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression plasmids for purification of recombinant Cdc14 enzymes were generated using the Gateway cloning system (Invitrogen). S ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant adenoviruses expressing the GRK2 shRNA were produced using BLOCK-iT™ Adenoviral RNAi Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2024Quote: ... The concentration of the purified recombinant proteins was determined using the BCA protein assay kit (Thermo Scientific), with bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2024Quote: ... The Dicer/TRBP enzyme was the mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 50 seconds elongation at 72°C with 1U of Pfu recombinant DNA polymerase (Thermo Fisher Scientific). The amplicon ORF45HA was cut with NheI/SmaI and inserted in pEGFP-C1 (Clontech) ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
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bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were generated in Sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen, USA). HEK293F cells were cultured under 5% CO2 at 37 °C and were used for transfection at a density of 2.5 × 106 cells/ml ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) and carried out in a total volume of 25 µL containing the following ...
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bioRxiv - Immunology 2023Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) with bovine serum albumin (BSA ...