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Citations for Thermo Fisher :
3501 - 3550 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Human IL-6 ELISA kit was from Life Technologies. FAN1KO HD patient fibroblasts were generated and received from Synthego ...
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bioRxiv - Genomics 2023Quote: ... and Human Brain Reference RNA (QS0611, Thermo Fisher Scientific) were used ...
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bioRxiv - Genomics 2023Quote: ... The Human Gene 2.1 ST Array platform from Affymetrix produced the data ...
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bioRxiv - Microbiology 2023Quote: ... or human TIA-1 (Assay ID 139893-Thermofisher scientific) or GFP targeting siRNA as a negative control (cat no ...
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bioRxiv - Cancer Biology 2023Quote: Microarray expression data (Affymetrix Human Gene 2.0 ST arrays) from Nicolle et al ...
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bioRxiv - Biophysics 2023Quote: ... media-washed human activator CD3/CD28 Dynabeads (Gibco, USA) were added at a 1:1 cell-to-bead ratio ...
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bioRxiv - Immunology 2023Quote: ... PE-Cy7 anti-human C27 (clone O323, ThermoFisher Scientific), AlexaFluor 700 anti-human CD38 (clone LS198-4-3 ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... previously coated with human type I collagen (Gibco, A1048301). ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal anti-human JAM-A (Invitrogen; #PA5-120157); rabbit polyclonal anti-CAR (kindly provided by Jeffrey M ...
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bioRxiv - Molecular Biology 2023Quote: Human HEK293T cells (ATCC) were maintained in DMEM (Gibco) supplemented with 10% heat inactivated FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL human IL-21 (Thermo Fisher Scientific), 50 ng/mL human IL-4 (Miltenyi) ...
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bioRxiv - Systems Biology 2024Quote: ... ANXA1 (rabbit anti-human, 1:1000, ThermoFisher Scientific, UK), phosphotyrosine-21 ANXA1 (rabbit anti-human ...
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bioRxiv - Cell Biology 2024Quote: ... or two siRNAs targeting human PLA2G15 (ThermoFisher s24296, s24297) using RNAiMax (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the human σ2 receptor was cloned into pcDNA3.1 (Invitrogen) mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... for human HTT and Alexa fluor 647 (Thermofisher # B40958) for mouse Htt ...
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bioRxiv - Neuroscience 2024Quote: ... AT8 human PHF-Tau (Thermo Scientific, MN1020, 1:250). The following secondary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-human CD3 (clone SP7, Thermo Fisher Scientific); sheep anti-human CD34 (#AF7227-SP ...
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bioRxiv - Bioengineering 2024Quote: Human dermal fibroblast (HDF) cells (C0135C; ThermoFisher Scientific, UK) were cultured in flasks with Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H+L) (Thermo Fisher Scientific) pre-coupled to Alexa Fluor 647 were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific) were added to the culture ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H + L) (Thermo Fisher Scientific) or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Human PA-1 cells were cultured in MEM (Gibco) supplemented with GlutaMAX ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (Telangana, India) with human suspension hepatocytes (HMCS1S, Gibco USA). 200 µL of cell suspension containing 2 x 106 hepatocytes/mL (>95% viability ...
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bioRxiv - Cell Biology 2024Quote: ... primary human islets were first dissociated with TrypLE (Gibco) prior to incubation with a mouse monoclonal anti-human NTPDase3 antibody (Ectopeptidases ...
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bioRxiv - Microbiology 2024Quote: ... or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific) antibodies were pre-coupled to Alexa Fluor 647 and used as secondary antibody in flow cytometry experiments ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant constructs of heavy and light chain were transfected at 1:1 ratio into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA). 4-5 days after transfection the antibodies were purified from the supernatant by protein A affinity resin (ProSep®-vA ultra ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000, Santa Clara, California and Human Brain Total RNA brRNA - ThermoFisher AM7962, Waltham, Massachusetts), as well as no-cell negative controls (1X TempO-Seq lysis buffer alone ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on the Clariom™ S Human Array to measure gene expression at >20,000 genes in the human genome (Affymetrix, Santa Clara, CA). Raw data generated from the arrays were read into R statistical software (version 3.5.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI–human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Systems Biology 2020Quote: ... An Ion Torrent PGM was used with the Hi-Q 400 template preparation and sequencing kit and a 318v2 sequencing chip (Thermo Fisher, MA). All the quality control steps were performed using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: The pFastBacNKI-his-3C-m6A-Tracer construct was used for creation of baculovirus according to the Bac-to-Bac system (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... of 8 mice were then resuspended in 10 ml DMEM supplemented with 10 % heat inactivated new born calf serum (HI NCBS, LifeTechnologies / Gibco, # 26010-074) and plated on two 10 cm dishes (Corning ...
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bioRxiv - Genetics 2021Quote: One microliter of GlobalFiler™ or GlobalFiler™ Express amplified product was added to 9.5 μL Hi-Di™ formamide (ThermoFisher Scientific) and 0.5 μL GeneScan™ 600 LIZ® size standard (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the SBT3.8 ORF was amplified from genomic DNA with a reverse primer including six His codons and first cloned into pCR2.1-Topo (Life Technologies, Carlsbad, CA). EcoRI sites from pCR2.1 were used for ligation into pART7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were then pooled and loaded onto chips and sequenced on Ion Torrent PGM using Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific). Fast-q or BAM files from sequencing runs were analysed in Geneious ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2019Quote: ... PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen, Carlsbad, CA, USA). CD16158F-CR and CD32131R-CR were subcloned into NcoI and MluI sites of the SFG retroviral vector ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...