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Citations for Thermo Fisher :
3301 - 3350 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: Hi-Def B8 medium was prepared by adding Hi-Def B8 aliquots (Defined Bioscience #LSS-201, San Diego, CA, USA) to DMEM/F12 (ThermoFisher #11320033) along with 1% antibiotic-antimycotic ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Microbiology 2022Quote: His-GspD and His-PilQOlut were expressed in Escherichia coli BL21 cells and purified according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD ...
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bioRxiv - Immunology 2022Quote: The extracellular region of the HLA-DPA1*02:01 and HLA-DPB101:01 chains were separately cloned into pMT\BiP\V5-His A (ThermoFisher scientific). The alpha chain construct harbors the acidic leucine zipper and terminates by a 6His-tag ...
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bioRxiv - Plant Biology 2022Quote: ... Each sgRNA was introduced into the shuffle vectors by overlap PCR with Q5 Hi-Fidelity polymerase followed by digestion of the original vector with DpnI (Thermo Scientific). A destination vector harboring UBQ10 promoter ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS and 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were added to previously equilibrated His-Pur™ Ni-NTA spin columns (0.2 mL resin bed, Thermo Scientific #88224) and incubated at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were added to previously equilibrated His-Pur™ Ni-NTA spin columns (0.2 mL resin bed, Thermo Scientific #88224) and mixed end-over-end at 4°C for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... Constructs were cloned into a Gateway pDest-CTD-His vector via a pDONR intermediate and BP /LR clonase (all reagents Thermo Fisher) following the manufacturer’s instructions (Hartley ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Genetics 2023Quote: ... The blots containing yeast Nap1 were treated similarly but incubated with mouse anti-HIS primary antibody (Thermo Fisher Scientific, Waltham, MA) and HRP-conjugated sheep anti-mouse secondary antibody (Kindle biosciences ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA fragment encoding the full-length Cckbr1 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The primers used here are listed in Extended Data Table 3 ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Microbiology 2023Quote: ... Extended products were purified by alcohol precipitation followed by dissolving in Hi-Di formamide and analysing in an ABI3700 automated DNA Analyzer (Applied Biosystems). Raw sequences are assumable and aligned using NCBI blast and submitted to NCBI databank for accession numbers ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Microbiology 2023Quote: ... or pCDNA SARS-CoV-2 ORF7a-V5/His (WT/H47Y; 0.75 μg) or empty vector using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... monocytes/macrophages (CD3-CD19-NKp46-CD11b+Gr1loLyC6int/hi) and neutrophils (CD3-CD19-NKp46- CD11b+Gr1+LyC6int) were counted using a Countess II Automated Cell Counter (Thermo Fisher) and then combined and processed as one sample per mouse ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were digested with EcoRI and XbaI and inserted into the mammalian expression vector pcDNA-V5-HIS-B (Invitrogen Corp.) that had been cut with the same enzymes ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μl of each diluted and multiplexed PCR product was then added to 10 μl of Hi-Di Formamide and 0.1 μl of GeneScan 500 LIZ Size Standard (Applied Biosystems Inc.) to prepare for genotyping ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were cultured in StemPro hESC SFM (Gibco) supplemented with 20ng/ml bFGF (R&D ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Microbiology 2021Quote: ... Human hepatocellular carcinoma cells (Huh-7; Thermo Fisher Scientific) and African green monkey kidney cells (Vero E6 ...
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bioRxiv - Immunology 2022Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Commercial pooled RNA (Quantigene Human Reference, QS0639, Life Technologies) and pooled human male DNA (G3041 ...
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bioRxiv - Molecular Biology 2021Quote: ... MEFs and human fibroblasts were cultured in DMEM (Invitrogen) supplemented with 10% FBS (Atlanta Biologicals ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher) at 1 bead per cell ...
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bioRxiv - Immunology 2022Quote: ... human recombinant Annexin V (5 μg/ml) (Invitrogen, #BMS306) or with the same amounts of their respective solvent (DMSO or H2O ...
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bioRxiv - Genomics 2020Quote: ... or Mouse anti-human GAPDH monoclonal (MA5-15738, Invitrogen). The secondary antibody was HRP conjugated Goat anti-Rabbit (NA934 ...
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bioRxiv - Cell Biology 2019Quote: Unlabeled LDL (1 mg) from human plasma (Thermo Scientific) was incubated with 50 nmol cholesterol (Top-Fluor cholesterol ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-human CD133 (Thermo Fisher Scientific, 1:1000) or rabbit anti-human Vimentin (Proteintech Group ...
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bioRxiv - Physiology 2019Quote: ... Antibodies: anti-human endoglin (Ref. H300; Thermo Fisher Scientific) and anti-mouse β-actin (Ref ...
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bioRxiv - Cancer Biology 2019Quote: ... Human recombinant bFGF and EGF were purchased from Invitrogen/Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml human recombinant EGF (Thermo Fisher) at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... Rabbit anti-human-Ki67 (1:20, Thermo Fisher Scientific), Rabbit anti-Chromogranin A (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg/mL human recombinant insulin (Thermo Fisher Scientific), 20 μg/mL ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... a secondary goat anti-human Alexa Fluor-555 (Invitrogen) was used as the target sites were bound with anti-human TCB after administration ...
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bioRxiv - Immunology 2021Quote: ... and 10% heat-inactivated human AB-positive serum (Invitrogen) at 37°C in 5% CO2 ...