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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 2005) Reverse transcription (RT) of total RNA (2 µg) was carried out using the High-Capacity cDNA Achieve kit (Applied Biosystems) following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... SARS-CoV-2 S trimer protein was biotinylated using the EZ-Link(tm) Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher) and then purified using Zeba(tm ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from HeP-2 cells at the indicated time using the GeneJET genomic DNA purification kit from Thermo Fisher® ...
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bioRxiv - Microbiology 2020Quote: ... and purified RNA from 2 ml of cells harvested immediately before and 5 min after stress using the RiboPure™-Bacteria Kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All plasmids were sequenced and transfected individually into Drosophila Schneider 2 (S2) cells using Lipofectamine 3000 Transfection reagent kit (Invitrogen, L3000008) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and fluorescent labeling was performed with the Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher), according to the manufacturer’s description ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated using 2 μg of RNA for each fraction with a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368814). qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... We converted 2 μg of RNA to cDNA with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Quantitative PCR on Quant Studio 12K Flex (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2022Quote: ... The expansion of the GGA library used the TOPO™ XL-2 Complete PCR Cloning Kit (Thermo Fisher Scientific, Vilnius, Lithuania) instead as it allowed the storage of the part maintaining the flanking BsaI cutting sites (RtGGA v1.1 ...
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bioRxiv - Developmental Biology 2022Quote: ... 10μM EdU was added for 2h to either day 2 or day 3 cultures and developed using the Click-IT EdU Alexa Fluor imaging kit (Life Technologies).
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bioRxiv - Genomics 2022Quote: ... Extracted RNA (1-2 µg) was used for reverse transcription to generate cDNA libraries with the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR was performed in the BioRad CFX96 Real-Time System using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Complementary DNAs were synthesized from 2 µg of total RNA using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Inc.) Real time Quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of TgTRPPL-2-smHA protein was performed using the Pierce HA Tag/Co-IP Kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... fresh media was added and after 30 min the cells were transfected with CRISPR reagents for insert 2 insertion using the Lipofectamine 3000 Transfection Kit (Invitrogen, L3000001) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and then 1-2 μg of total RNA was used to prepare cDNA using high-capacity cDNA Reverse Transcriptase kit (Applied Biosystems). Quantitative PCR was performed using SYBR Green master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Typhimurium-infected BMDMs was extracted 2 h post infection using the Qiagen RNAeasy kit and cDNA was synthesized with SuperScript III (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the final concentrations of all libraries (Supplementary Table 2) were measured using the Qubit® dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were normalised to 2 µg/µl in radioimmunoprecipitation assay (RIPA) buffer using the Qubit® Protein Assay Kit (ThermoFisher Scientific) prior to processing as described in McVeigh et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription was performed on 2 μg of DNase-treated RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 00777852) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA was quantified using an Invitrogen Qubit Fluorometer (version 2) with a Qubit™ dsDNA HS Assay Kit (ThermoFisher catalog #Q32851).
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bioRxiv - Developmental Biology 2019Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) labeling was performed using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Invitrogen Cat#C10340) following manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: Single cells were stained with 4 μM Ethidium homodimer-1 and 2 μM Calcium AM (ThermoFisher LIVE/DEAD Viability Kit L3224) in the dark at room temperature for 20 min ...
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bioRxiv - Plant Biology 2019Quote: ... Reverse transcription reaction for cDNA synthesis was performed from 1 to 2 μg of total RNA treated with DNAse I and with the cDNA First Strand cDNA Synthesis kit (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA (2 μg) was used as a template for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). For qRT-PCR ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was performed as follows: 2 μg of messenger RNA (mRNA) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized by reverse transcription using 2 μg of total RNA with a high-capacity cDNA Reverse Transcription Kit (Applied Biosystems). Primers were designed by identifying a sequence stretch with a unique sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA and protein-free DNA samples were obtained by adding RNase and proteinase K to 50μl of sample at 65°C for 2 hours and purifying the DNA using PCR purification kit (Thermo fisher scientific). Purified DNA was run on 1.5% agarose gel to check efficiency of chromatin shearing ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were stained between 2 and 4 hours after extraction using a LIVE/DEAD® Cell Imaging Kit 488/570 (Invitrogen), and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Dried peptide mix was resuspended in 2% TFA and fractionated using Pierce High pH Reversed-Phase Fractionmation kit (84868, ThermoFisher Scientific). Each fraction was vacuum-dried and resuspended in 25 μl 0.1% TFA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA concentration was measured using Nanodrop and cDNA was synthesized from 2 μg total RNA with the SuperScript VILO kit (Thermo Fisher). Primers targeted Grm3 ...
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bioRxiv - Evolutionary Biology 2020Quote: We subjected 2 μg total RNA to cDNA synthesis using a HighCapacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Waltham, MA, USA). The cDNA was diluted 16-fold and 5 μl was subjected to real-time quantitative PCR using gene-specific primers (listed in Supplemental Table S9 ...
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bioRxiv - Immunology 2021Quote: ... or 500-1000 pmol of sgRNA delivered into 2*106 cells per electroporation using the Neon 100 µL Kit (Invitrogen, MPK10096). Electroporated Lin-cells were kept in culture for two days in cRPMI with cytokines before sequencing to allow for the CRISPR events to occur ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total RNA (2 µg) from each sample was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: EdU (5-ethynyl-2′-deoxyuridine) detection was performed with the Click-iT EdU Alexa Fluor 555 Imaging Kit (Thermo-Fisher Scientific). EdU was diluted in DMSO to a final concentration of 10 mM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The cDNA was synthesized from 2 μg of total RNA using Maxima first strand cDNA synthesis kit for RT-qPCR with dsDNase (Thermo Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: 2×105 cells were lysed in 50 μl RIPA buffer and protein amount was assessed using BCA assay kit (Thermo Scientific). Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: The Fab fragments of Clone 2 and Clone 6 were generated from full length IgGs of Clone 2 and Clone 6 using a commercial PierceTM Fab Preparation Kit (Thermo Fisher). All procedures were performed following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription of 2 μg of total RNA to single-stranded cDNA was performed using High Capacity cDNA kit (Applied Biosystems). To quantify PROS1 transcripts ...
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bioRxiv - Genomics 2019Quote: ... sibericum genomic DNAs were extracted from 2×1010 purified particles using the the PureLink Genomic DNA Extraction Mini Kit (Thermo Scientific) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of DNase treated total RNA was reverse transcribed into cDNA using the cDNA Reverse Transcription Kit (Invitrogen, Cat #: 4374966). PCR reactions were then set up using Power SYBR Green PCR Master Mix (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The resulted PCR products were extracted from a 2% agarose gel and further purified with GeneJET TM Gel Extraction Kit (Thermo Scientific) as stated by the protocol of manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were detected by differential staining with annexin V (which stains apoptotic and necrotic cells) and propidium iodide (PI) (which stains necrotic cells only) using a Annexin-V Apoptosis Kit 2 (Invitrogen V13241) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and the SARS-CoV-2 spike gene was reverse-transcribed and amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
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bioRxiv - Immunology 2021Quote: ... then cDNA was prepared using 2 μg of RNA and the High capacity RNA to cDNA kit (Applied Biosystems cat#4387406) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...
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bioRxiv - Microbiology 2022Quote: cDNA synthesis and quantitative PCR (qPCR): 2 µg of RNA was reverse transcribed into cDNA using the Superscript III kit (Thermo Fisher) as per the manufacturer’s instructions ...