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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Zika Virus DIII Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 IU of Moloney murine leukemia virus RNase H+ reverse transcriptase solution (Thermo Fisher Scientific), and appropriate buffer containing deoxynucleoside triphosphates (dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated total RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo-dT primers ...
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bioRxiv - Microbiology 2021Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in Leibovitz’s L15 media (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: Compound dilutions for virus treatment were performed in Minimum Essential Media (MEM) purchased by Gibco.
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bioRxiv - Microbiology 2019Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075). HSV-1 expressing the FLAG-tagged N-terminal extension of UL54 virus were reconstituted and titrated on Vero 2-2 cells50 ...
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bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... expanded PBMCs were transduced with non-integrating Sendai reprogramming virus (Cytotune 2.0, Thermo Fisher, A16517) according to manufacturer’s instructions added to the Expansion Media ...
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bioRxiv - Genomics 2020Quote: ... endometrium tissues fibroblast cells were transfected with a Sendai virus reprogramming kit (Life Technologies, A16517). The transfected cells were then plated onto Matrigel-coated culture dishes according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... The viral DNA samples were prepared for quantification by treating the virus with DNaseI (Invitrogen) and Proteinase K ...
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bioRxiv - Microbiology 2020Quote: The nasal swab was initially stored in virus transport medium [Hank’s Balanced Salt Solution (Gibco) containing 2% FCS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... qPCRs were set up using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher) and run on an ABI 7500 Fast Real-time PCR System (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... the virus inoculum was previously activated with trypsin (200 μg/ml, Gibco Cat. 27250-18) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Virus was washed and eluted in Hank’s balanced salt solution (Gibco™, Dublin, Ireland) containing 0.014% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: Purified virus stocks were diluted in lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific) and separated by SDS-PAGE (NuPAGE Novex 10% Bis-Tris gel ...
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bioRxiv - Neuroscience 2022Quote: ... and reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time polymerase chain reaction (PCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the precipitated virus pellet was resuspended with 1.1 mL of Opti-MEM (Gibco #31985062) by pipetting and vortexing until fully dissolved ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with TaqMan Fast Virus 1-Step Master Mix (Invitrogen, cat. 4444434) in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... vRNA was reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 instrument (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Virus samples at variable dilutions (1:10 to 1:40,000) were mixed with FluoSphere (Invitrogen) beads (170nm ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus pellet and cell RNA was isolated using TRIzol according to the manufacturer’s protocol (Ambion) and DNase treated ...
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bioRxiv - Microbiology 2023Quote: ... Virus-depleted and concentrated cell SN (in PBS) was added to anti-PA (Thermo Fisher) coated beads and incubated overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
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bioRxiv - Microbiology 2024Quote: ... The virus genome was further amplified by PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) with 10 μL cDNA in each 50 μL reaction and MBTuni-12/13 (5′-ACGCGTGATCAGTAGAAACAAGG ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentiviruses were obtained by transient transfection of the psPAX2 packaging plasmid and pMD2.G envelope plasmid in HEK293T cells by using Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA). Transfection efficiency was validated by western blotting ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Catalog Numbers 4444432). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from purified virus using the GeneJET genomic DNA purification kit (Thermo Fisher), and the fidelity of the changed regions was verified by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus (M-MLV; Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... wt Flock House virus (FHV) was generated by transfecting S2 cells with pMT plasmid vectors (Invitrogen) containing respective genomes (NC_004146 for RNA 1 ...
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bioRxiv - Biochemistry 2019Quote: ... 293FT producer cells with ViraPower packaging mix (pLP1, pLP2, and pLP/vesicular stomatitis virus; ViraPower; Invitrogen) to generate the lentivirus according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times and supplemented with virus production serum-free media (VP-SFM, Gibco) containing 0.6 µg/ml trypsin ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Clarified virus working stock supernatant was overlaid onto a 25% sucrose-NTE (100nM NaCl (ThermoFisher Scientific), 10mM Tris (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Reprogramming to pluripotency was carried out using the CytoTune 2.0 sendai virus kit (Thermo-Fisher Scientific) to overexpress four transcription factors (OCT4 ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... unbound virus was removed by washing three times with PBS (Life Technologies, Carlsbad, California, United States). Next ...
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bioRxiv - Microbiology 2020Quote: ... The virus in the growth medium was recovered by Polyethylene Glycol 8000 (PEG-8000, Fisher Scientific) precipitation ...
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bioRxiv - Biophysics 2020Quote: Virus was attached to a gold-coated probe using dithiobis succinimidyl propionate (DSP) linkers (Thermo Scientific). Dithiobis succinimidyl propionate (DSP ...
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bioRxiv - Cell Biology 2020Quote: ... and sendai-virus iPSCs were cultured on matrigel-coated plates (Geltrex™ LDEV-Free, ThermoFisher Scientific) in Essential 8™ medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen) with oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...