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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...
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bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal eight-His tag and subcloned into the pPIC9K vector (Invitrogen). Mutants were generated with Quikchange XL kit (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated RNA baits were used in a ratio 1:12 to Hi-C libraries (25 ng biotinylated RNA baits per 300 ng of Hi-C library) and supplemented with 30 units SUPERase-In (Ambion). Biotinylated RNA baits for capture DNA-Seq were used in a ratio of 1:3.33 (300 ng RNA baits per 1,000 ng genomic DNA library ...
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bioRxiv - Plant Biology 2021Quote: ... fused with a six-HIS tag at the C-terminus were expressed using the Bac-to-Bac baculovirus expression system (Invitrogen) in High Five cells at 22 °C as reported previously 23 ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Cell Biology 2022Quote: The cDNA coding for fibulin1C (Uniprot number P23142-4) with a C-terminal 6x His tag was custom-synthesized by Invitrogen and transiently transfected in HEK293 T cells using polyethylenimine in OPTIMEM (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: The SARS-CoV-2 NTD (residues 13-303) or RBD (residues 319-541) with a C-terminal His tag was cloned into a modified pFastBac vector (Invitrogen) that encodes a melittin signal peptide before the NTD or RBD ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant obtained after the centrifugation of the lysate at 9000 rpm for 25 minutes at 4°C was subjected to overnight binding with Hi-bind Ni-NTA agarose beads (Invitrogen). The beads were washed 4-5 times with wash buffer (20mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Infected Sf9 cells were grown in spinner culture for 48–96 h at 27°C and His-tagged protein-purified using Ni2+-NTA agarose (Invitrogen) according to standard procedures ...
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bioRxiv - Microbiology 2020Quote: ... fused to a Gly-Ser-Gly linker and a Myc epitope tag into the EcoRV site of pEF1 V5 His C (Invitrogen) using NEB HiFi Assembly Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal 8-His tag and subcloned into the pPIC9K (A1ACR1, HfACR1 and AlACR2) or pPICZα (AlACR3) vector (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was clarified for 10 min at 4°C at 16k rcf and incubated with 60 μl Dynabeads HIS-Tag isolation (10104D, Invitrogen) for 2 hour with rotation at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
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bioRxiv - Cell Biology 2024Quote: All eukaryotic expression plasmids for pUL71 and pUL71 homologues were generated in the backbone of pEF1/Myc-His C expression vector (Invitrogen) by cloning using restriction enzymes ...
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mL EGM-2 medium supplemented with an additional 75 ng/mL VEGF (Gibco, PHC9394) was added to each well ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Cancer Biology 2021Quote: 500 ng to 2000 ng of Hi-C library DNA or genomic DNA library were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 μl dH2O ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Developmental Biology 2022Quote: ... Biotin pulldown was performed by adding an equal volume of Hi-C sample with Dynabeads MyOne Streptavidin T1 beads (Invitrogen, 65602) and incubated by 15 minutes with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
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bioRxiv - Developmental Biology 2019Quote: HEK293 (Flp-In™-293 Cell Line, Thermo Fisher Scientific) were grown in DMEM supplemented with 10% FBS ...