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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573) were maintained at 37° C and 5% CO2 in 1X DMEM medium (Thermo Scientific; Waltham ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Biochemistry 2023Quote: U2 OS and HEK293-T cells were grown at 37 °C in 5 % CO2 in DMEM low glucose (Gibco 11885) supplemented with 10 % heat inactivated FBS (F4135 ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... anti-human C-myc (10µg/ml, clone 9E10; Thermo scientific), anti-human α-tubulin (diluted at 1/100 ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Molecular Biology 2021Quote: ... ELMSAN1 TRERF1 DKO HEK293 cells were generated from wild-type Flp-In T-REx HEK293 cells (Invitrogen, R78007) by the Center for Advanced Genome Engineering (CAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat ...
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bioRxiv - Molecular Biology 2023Quote: Drosophila S2 cells were cultured at 23°C in Schneider’s media with 10% Gibco HI-FBS (Thermofisher) and 1% Gibco Antibiotic-Antimycotic (Thermofisher) ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific). After incubation ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Biophysics 2020Quote: HeLa and HEK293 cells were cultured at 37°C in an atmosphere of 5% CO2 in air in DMEM (Gibco, #10566024) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: HEK293 Flip-In TREx cells (Thermo Fisher) were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher ...
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bioRxiv - Biochemistry 2020Quote: HEK293-F cells (FreeStyle™, ThermoFisher #R79007) were maintained in suspension culture according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T or HEK293 GnTI- cells (Thermo Fisher) were incubated with 200 nM (monomer concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Flp-In T-REx 293 (Invitrogen), was grown at 37°C in DMEM high glucose supplemented with 5% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: HEK293-Flp-In T-Rex cells (Invitrogen) were cultured in high glucose DMEM containing 10% FBS at 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells grown in DMEM media (Gibco) supplied with 10%FBS and 2mM L-Glutamine were seeded onto coverslips in 24 well plates and allowed to adhere and grow overnight at 37°C incubator supplied with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293-FT cells were purchased from Invitrogen. U87MG cells were Purchased from the ATCC and cultured as previously described (Sabag et al. ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells was cultured in DMEM (Invitrogen) supplemented with 10% FBS at 37 °C and 5% CO2 environment ...
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bioRxiv - Cancer Biology 2022Quote: ... Flp-In T-REx HEK293 cells (ThermoFisher) were cultured in DMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: Flp-In 293 cell line (HEK293; ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genetics 2023Quote: HEK293 cells (Thermo Fisher, Waltham, MA, USA) were cultured at 37°C in Dulbecco’s Modified Eagle’s Medium containing high levels of glucose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in DMEM (Gibco) containing penicillin (100 U/mL ...
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bioRxiv - Microbiology 2024Quote: HEK293 cells were maintained in DMEM (Invitrogen) supplemented with 10% FBS (Cytiva) ...
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bioRxiv - Genetics 2024Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: Flp-InTM T-RexTM HEK293 (Thermo Fisher), Flp-InTM T-RexTM HeLa (gift from S ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...