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Citations for Thermo Fisher :
301 - 350 of 5279 citations for TNF beta Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and TNF-α were quantified using a 9-plex Procartaplex assay (PPX-09, Thermo Scientific). In short ...
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bioRxiv - Microbiology 2022Quote: ... culture medium was collected and analyzed for murine TNF by ELISA kit (Invitrogen, 88-7324) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... A beta release of the Collibri 3’ mRNA Library Prep Kit (Invitrogen) was used to prepare libraries ...
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bioRxiv - Bioengineering 2023Quote: ... and housekeeping gene for beta tubulin (TUBB) (#Hs00742828_s1, ThermoFisher Scientific, Waltham, MA). TaqMan master mix (4444556 ...
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bioRxiv - Immunology 2022Quote: ... Pre-designed gene expression assay for beta-Actin (Applied Biosystems™, Mm01205647_g1) was used for normalization ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% penicillin/streptomycin (Pen/Strep) and 0.5 μM beta-mercaptoethanol (Life Technologies). Medium was changed every second day and cells were passaged at 1:3 or 1:5 ratio ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... L19-murine TNF (L19-mTNF) gene was cloned into the mammalian cell expression vector pcDNA3.1(+) (Invitrogen) by HindIII/NotI restriction sites ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse TNF-α and IL-6 ELISA Ready-SET-Go! kits (Affymetrix, San Diego, CA, USA) were used for the detection of tumor necrosis factor α (TNF-α ...
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bioRxiv - Immunology 2022Quote: ... TNF concentrations in the supernatants were measured by ELISA (Fisher Scientific, Waltham, MA, Cat# 88-7346).
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bioRxiv - Immunology 2023Quote: ... and mouse TNF-α ELISA kit (#88-7324-88) were purchased from Invitrogen (Carlsbad, CA, USA). The capture antibody for IFN-β ELISA (#sc-57201 ...
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bioRxiv - Immunology 2023Quote: ... LUVA supernatants were assayed for IL-6 and TNF (Invitrogen, #88-7066-88, #88-7346-88). β-hexosaminidase was measured in fresh supernatants by transferring 20µl sample/well to a 96 well plate (Sarstedt ...
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bioRxiv - Biochemistry 2020Quote: ... Beta-galactosidase and luciferase activities were measured using Dual-Light System (Applied Biosystems).
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bioRxiv - Bioengineering 2021Quote: ... Beta-cyclodextrin hydrate (99%) and didodecyldimethylammonium bromide (99%) were purchased from Acros Organics. Cetyltrimethylammonium bromide (≥98% ...
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bioRxiv - Immunology 2023Quote: ... cDNA was amplified using the Oncomine TCR Beta-LR Assay (Thermo Fisher Scientific) and barcoded library were prepared using Ion Ampliseq library kit Plus (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
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bioRxiv - Immunology 2022Quote: Murine TNF-α and IL-1β were quantified in cell supernatants using ELISA™ Kit from Invitrogen and human IL-1β using Instant ELISA™ Kit from Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Protein lysates were separated by semi-dry western blot (anti-TNF, 1:1000, Invitrogen (14-7321-85); anti-GAPDH ...
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bioRxiv - Immunology 2022Quote: ... and PE-Cy7 anti-mouse TNF-α (1:80) in IC Permeabilization Buffer (eBioscience, Thermo Fisher, Inc.) for 60 min at room temperature in dark ...
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bioRxiv - Immunology 2023Quote: ... and serum IL-6 and TNF-α levels were measured using a commercial ELISA kit (Thermo Fisher). Serum CD5L levels were measured using ELISA ...
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bioRxiv - Physiology 2023Quote: ... Subsequently tissues and serum were analyzed for cytokine levels using standard ELISA kits: TNF (88-7324: Invitrogen); IL-6 (R&D Systems DY406) ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Microbiology 2020Quote: ... a 6x stock solution of the beta-lactamase substrate CCF2-AM (Thermo Fisher, K1032) was added to the media over 10 T1/2 cells so that the final concentration of CCF2-AM was 1x ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated in beta actin primary antibody (1:1000, Thermo Fisher Scientific, Cat. #MA515739) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... We manipulate concentration of transforming growth factor beta-1 (TGF-β, Invitrogen, CA, USA) between source and sink channels to develop chemical gradients in the center channel ...
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bioRxiv - Bioengineering 2022Quote: ... mouse monoclonal beta-catenin Alexa Fluor 647-conjugated antibody (Thermo Fisher Scientific; 1:100); mouse monoclonal VE-cadherin Alexa Fluor 488-conjugated antibody (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Each reaction was normalised to a beta-2-microglobulin (B2M) control (Hs00984230_m1; Applied Biosystems). For quantifications using SYBR-green ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3.3 nM of 5-chloromethylfluoresecein di-beta-D-galactopyranoside (CMFDG) (Invitrogen, Waltham, MA, USA) was added to the reaction solution ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies include Anti-beta tubulin (1:500, mouse monoclonal, 2 28 33, Invitrogen), Anti-Hec1 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and beta Actin mouse Monoclonal Antibody (AC-15) (# Cat. No. MA1-91399, Thermo Scientific). The secondary antibodies used were Anti-rabbit IgG (H+L ...
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bioRxiv - Biophysics 2023Quote: Primary antibodies used were: Mouse-beta tubulin monoclonal antibody (Thermo Fisher Scientific, 32–2600), Rabbit-anti-Tom20 polyclonal antibody (Abcam ...
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bioRxiv - Genetics 2023Quote: ... 0.1 mM beta-mercaptoethanol,100 U/ml penicillin and 100 mg/ml streptomycin (Gibco)) on SNL-HBP feeder cells ...
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bioRxiv - Cell Biology 2024Quote: Primary pancreatic beta cells were dispersed from the purified islets using trypsin (GIBCO, 25200072) digestion and cultured in RPMI 1640 medium (GIBCO ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with primary antibody directed against Aβ (Invitrogen, beta Amyloid (1-40) Polyclonal Antibody ...
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bioRxiv - Immunology 2023Quote: ... TNF-α and IL-17 were measured in the culture supernatant using a cytokine measuring kit (Invitrogen, USA) [33].
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Neuroscience 2020Quote: Cytokines released by primary microglia in the culture medium were quantified by ELISA using the following commercial kits according to manufacturer’s instruction: Mouse TNF alpha Uncoated ELISA kit (88-7324-22, Invitrogen), Rat IL-6 DuoSet ELISA (DY506 ...
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bioRxiv - Bioengineering 2019Quote: ... The resulting VH-(G4S)3-VL ScFv fragment was further fused at the N-terminus of the murine TNF gene through a S4G-linker and the final construct VH-(G4S)3-VL-(S4G)3-TNF was then cloned into the mammalian expression vector pcDNA3.1 (+) vector (Invitrogen). A VH-(G4S)3-VL ScFv fragment specific for hen egg lysozyme (KSF)(34) ...
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bioRxiv - Microbiology 2020Quote: ... In particular IFN-γ and TNF-α were evaluated by Mouse Custom ProcartaPlex 9-plex (Invitrogen, Thermo Fisher Scientific) and normalized at 2500 ug/ml of quantified proteins in lung supernatants.
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bioRxiv - Microbiology 2020Quote: ... In particular IFN-γ and TNF-α were evaluated by Mouse Custom ProcartaPlex 9-plex (Invitrogen, Thermo Fisher Scientific) and normalized at 2500 ug/ml of quantified proteins in lung supernatants.
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... this is equivalent to total of 190,000 cells) in the morning and stimulated overnight with 10 ng/mL TNF (PHC3015, Invitrogen). The RBC control was prepared by culturing a 2% haematocrit suspension in RPMI medium overnight ...
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bioRxiv - Biophysics 2019Quote: ... we fluorescently labeled beta actin monomers using CellLight Reagent BacMam 2.0 GFP (by Life technologies). Additionally ...
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bioRxiv - Pathology 2019Quote: ... RCSC samples were then incubated with beta-amyloid (36-6900; 1:100; Thermo Fisher Scientific), αB-crystallin (Ab151722 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were immunostained with anti-beta-3 Tubulin (Invitrogen, cat. no. 2G10-TB3, 1:500) in 2.5% NDS overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... and re-probed with a beta actin-HRP conjugated antibody (Fisher Scientific, MA515739HRP 1:1000) as described above ...
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bioRxiv - Microbiology 2023Quote: ... Tubulin was stained for 1 h with a monoclonal antibody anti-beta tubulin antibody (Invitrogen) at a final concentration of 2 μg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken TNF-α (LITAF, assay ID number Gg03364359_m1) and the reference gene chicken GAPDH (assay ID number Gg03346982_m1) (Thermofisher Scientific). The PCR cycle parameters were an initial denaturing step at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 s and 60 °C for 1 min ...
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bioRxiv - Neuroscience 2021Quote: The supernatant from this homogenization step was used to evaluate levels of TNFα using the mouse TNF alpha High Sensitivity ELISA Kit according to manufacturer’s instructions (Invitrogen, BMS607HS). Briefly ...