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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and Spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcribed RNA of SARS-CoV-2 N-gene was synthesized with MEGAscript T7 (Thermo Fisher Scientific) using PCR product generated from SARS-CoV-2 WA1 cDNA and primers N0.5_F and N3-5_R (Eurofins ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 coding sequences were inserted using the Gateway LR Clonase II Enzyme Mix kit (Invitrogen, CA), yielding plasmids pLCPD-S ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were probed with rabbit-anti-SARS spike (Invitrogen, PA1-411-1165), mouse-anti-SARS-CoV-2 spike (GeneTex 1A9) ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S 2P ectodomain and RBD constructs were produced by transient transfection of 500 mL of Expi293 cells (Thermo Fisher) and purified from clarified transfected cell supernatants four days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction of SARS-CoV-2 infected A549-ACE2 cells was performed by TRIzol (Thermo Fisher Scientific, 15596-026) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S protein Omicron genes were synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific). Pseudotyped retroviral particles were produced in HEK293T cells as described previously (32) ...
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bioRxiv - Microbiology 2021Quote: ... and 0.06 μg/ml or vehicle and 2 h later infected with SARS-CoV-2 (multiplicity of infection (MOI) = 0.01 and 0.1) in serum-free DMEM (Thermo Fisher) for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... Taqman gene expression assays for N2 and N3 regions of the SARS-CoV-2 N gene were made (ThermoFisher) following CDC specifications (Division of Viral Diseases ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Immunology 2021Quote: ... SARS-COV-2.SctΔ19 Wuhan plasmid was amplified by PCR with Phusion DNA polymerase (Thermo Scientific, ref F-549S) and the following primers ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were transfected with GFP-Flag or SARS-CoV-2 S-Flag (1.5 μg/ml) constructs using Lipofectamine 3000 reagent (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher). Streptavidin-BV510 (BD ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were transfected with GFP-Flag (VB200507-2985cmv) or SARS-CoV-2 S-Flag (VB200507-2984jyv) (1.5 μg/ml) constructs using Lipofectamine 3000 reagent (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then FISH-stained against SARS-CoV-2 ORF1a mRNA using ViewRNA mRNA FISH assay according to the manufacturer’s instructions (ThermoFisher) with some modifications ...
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bioRxiv - Microbiology 2022Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and cloned using SacI and ApaI in the pCR3 SARS-CoV-2-SΔ19 expression plasmid (13) using Gibson Assembly (ThermoFisher). All constructs were verified by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Microbiology 2023Quote: ... and the insect cell codon-optimized RBD at residues 330 to 521 of SARS-CoV-2 S protein (GenBank accession no. MN908947.3) was cloned into a pFastBac plasmid (Invitrogen). For the RBD-LTA fusion protein expression ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... were coated with 50 μL per well of 2 μg/mL Spike trimer or RBD in 1 x PBS (Fisher Scientific, 11510546) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 5 × 106 HEK 293T cells were co-transfected with pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 Spike (D614G) plasmid or its derivatives (B.1.1.7 and B.1.617) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Supernatants containing pseudotyped viruses were harvested 48 h post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
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bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
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bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
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bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Microbiology 2021Quote: ... cells in each well were infected with 2000 TCID50 SARS-CoV-2 pseudovirus in 100 µL of culture medium containing 2% FBS (Gibco, Cat. No. 10099141C). Culture medium was refreshed every 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... ERCC spike mix 2 (Ambion) was added to the RNA ...