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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: RNA from SARS-CoV-2-infected organoids was extracted by adding 750 μl TRIzol (Thermo Fisher Scientific), incubating at 55 °C for 5 min and then adding 150 μl chloroform ...
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bioRxiv - Immunology 2021Quote: ... Immunohistochemistry (IHC) was performed using a monoclonal antibody specific to SARS-CoV-2 Nucleocapsid (clone B46F; ThermoFisher) per previously reported methods [30] ...
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bioRxiv - Immunology 2021Quote: ... lung tissue of mice challenged with SARS-CoV-2 was homogenized in TRIzol reagent (Life Technologies, 15596018) and total RNA was isolated using a PureLink RNA Mini kit (Life Technologies ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in Vero E6 cells in Opti-MEM I (Invitrogen, Cat. # 51985091) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... ChAdOx1 SARS CoV-2 S viral vectors were rescued in T-REx™ cells (Invitrogen, Cat. R71007), a derivative of HEK293 cells which constitutively express the Tet repressor protein and prevent antigen expression during virus production ...
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bioRxiv - Microbiology 2020Quote: ... Gene specific primers to SARS-CoV-2 (Wuhan v1, NSP14) and SYBR green master mix (Applied Biosystems) were used to amplify viral RNA and 18S rRNA primers were used to amplify cellular RNA using the QuantStudio 6 Flex RT-PCR system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2021Quote: The SARS-CoV-2 S ectodomains were produced in 200 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A variant-specific RT-qPCR with the TaqMan™ SARS-CoV-2 kit Mutation Panel (Applied Biosystems) was used to screen the VOC/VOI mutations S_L452R and S_T478K following manufacturer ‘s protocol.
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bioRxiv - Microbiology 2021Quote: Mouse tissue from SARS-CoV-2 infected mice was homogenized using glass beads in TRIzol Reagent (Invitrogen). Equal volume of 100% EtOH was mixed with the TRIzol homogenate and processed using Direct-Zol RNA MiniPrep Kit (Zymo ...
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bioRxiv - Microbiology 2020Quote: Milling of SARS-Cov-2 infected cells was carried out using a Scios DualBeam cryoFIB (ThermoFisher Scientific) equipped with a PP3010T transfer system and stage (Quorum Technologies) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in VeroE6 cells in Opti-MEM I (Invitrogen, Carlsbad, CA, USA) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 S ectodomains were produced in 100 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: Primary antibodies used in immunohistochemical assays with dilutions: SARS-CoV-2 nucleocapsid (Invitrogen #PA5-81794; 1:1000), CD3ɛ (CST #99940 ...
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bioRxiv - Bioengineering 2023Quote: ... Gene-specific primers to SARS-CoV-2 (Wuhan v1, NSP14) and SYBR green master mix (Applied Biosystems) were used to amplify viral RNA ...
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bioRxiv - Cell Biology 2023Quote: ... SARS-CoV-2 quantification was performed employing the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Inc.) with indicated primers (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Immunology 2021Quote: ... The proteins were transferred to nitrocellulose membrane and probed with polyclonal rabbit serum against SARS-CoV S1 domain (Thermofisher, Cat No. PA5-81798 and PA5-81795), monoclonal antibodies I1 (8G5F11 ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 variants were identified by the targeted sequencing approach with the Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... spike glycoproteins were produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Cell Biology 2020Quote: ... His-tags were removed with AcTEV protease (Invitrogen) overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Immunology 2024Quote: Dynabeads His-Tag Isolation and Pulldown beads (ThermoFisher) were washed once with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... anti-6*his tag (Thermo Fisher Scientific,11533923), anti-myc tag (Abcam ...
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bioRxiv - Immunology 2021Quote: RBDwt of SARS-CoV-2 and mutant RBDs (RBDK417N, RBDE484K, RBDN501Y, RBDK417N/E484K/N501Y, and RBDL452R/E484Q) were expressed using Expi293F cells (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 were mixed in a ratio of 0.7:0.7:0.4 with Opti-MEM® media (Gibco) to give 275 μL ...
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bioRxiv - Microbiology 2022Quote: ... and spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into HEK293T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Cell Biology 2021Quote: ... SARS-CoV-2 titration was obtained using a TaqMan 2019nCoV assay kit v1 RealTime-PCR (Thermo Fisher, Italy).
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bioRxiv - Biochemistry 2021Quote: The gene encoding the PLpro (also known as nsp3) protein from SARS-CoV-2 (YP_009725299.1) was also synthesized commercially (Invitrogen) and cloned into the pSUMO1 vector with N-terminal 8xHis conjugated with yeast SUMO ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: SARS-CoV-2 total viral RNA was extracted from Vero cells (ATCC CCL-81) cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from Vero CCL81 cells infected with VSV-SARS-CoV-2-SΔ21 using Trizol (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Immunology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using Wuhan/WIV04/2019strain (GISAID accession ID ...