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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Cell Biology 2024Quote: ... human recombinant insulin (ThermoFisher) was added at 100 nM for 30 minutes and Smoothened agonist (SAG ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant PAK6 (ThermoFisher, #PR5307A) was diluted in blocking buffer and incubated with the arrays at room temperature (RT ...
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bioRxiv - Molecular Biology 2020Quote: ... zebrafish larvae at 3 dpf were incubated with LysoTracker Red DND-99 (Life Technologies) which contains fluorescent acidotropic probes for labeling and tracking of acidic organelles ...
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bioRxiv - Developmental Biology 2019Quote: ... 20 zebrafish larvae digestive tracts were dissected and pooled into 1mL TRIzol (ThermoFisher, 15596026). mRNA was then isolated with isopropanol precipitation and washed with 70% EtOH ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated in zebrafish medium containing 5μM DAF-FM DA (Life Technologies, D23842) for 30 minutes in the dark at 28ºC ...
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bioRxiv - Neuroscience 2021Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Neuroscience 2020Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Biophysics 2022Quote: Mfsd2a isoform B from zebrafish (Uniprot Q6DEJ6) was subcloned in the pFasbac1 vector (Invitrogen) with a N-terminal-10Xhis tag ...
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bioRxiv - Developmental Biology 2021Quote: ... xenopus (Sox18) or zebrafish (Nr2f2) genes were cloned into a pME Gateway vector (Invitrogen) and then recombined with the zebrafish ubi promoter68 ...
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bioRxiv - Neuroscience 2021Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Neuroscience 2019Quote: ... zebrafish larvae were immobilised in 2% low melting point agarose (Fisher Scientific, BP1360-100), placed on a glass coverslip and submerged in fish water ...
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bioRxiv - Molecular Biology 2020Quote: Adult zebrafish liver and heart tissue were homogenized in NP40 lysis buffer (ThermoFisher, FNN0021). Protein concentrations were estimated using BCA (Pierce 23225 ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from cells or zebrafish embryos using TRIzol™ (ThermoFisher, 15596081) reagent according to manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: Whole-mount staining of live zebrafish larvae was performed using Yo-Pro-1 (Invitrogen) to observe neuromast hair cells ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from zebrafish intestinal tissue using TRIzol™ Reagent (Invitrogen Cat# 15596026) as follows ...
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bioRxiv - Immunology 2024Quote: ... RNA was isolated from zebrafish larvae using TRIzol according to the manufacturer’s instructions (Invitrogen) and cDNA was made with iScript RT Supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco and used at the indicated concentrations ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Cells were lysed by sonication and recombinant proteins purified by Ni2+ affinity chromatography in Ni-NTA agarose (Thermo Fisher) using 50 mM TrisHCl/500 mM NaCl/10 mM imidazole [ph 8.0] as binding buffer ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Molecular Biology 2024Quote: ... Purity of recombinant proteins was verified by SDS-PAGE with staining by GelCode Blue stain reagent (Thermo Fisher scientific).
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bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant lentivirus used to infect SW620 cells for protein expression was generated by the ViraPower Lentiviral Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... GPI-RBD-hGM-CSF recombinant fusion protein and GPI-hIL-12 were affinity purified from CHO-S cells (Invitrogen) stably expressing the proteins using anti-human GM-CSF (clone BVD2-21C11 ...
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bioRxiv - Cell Biology 2020Quote: ... 24hpf Tübingen zebrafish embryos were harvested and homogenized using a micropestle in TRIzol reagent (ThermoFisher), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... before resuspension in Zebrafish Neural Medium (ZNM) consisting of Leibovitz L-15 medium (Gibco-11415049), 1X N-2 Supplement (Gibco-17502048) ...
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bioRxiv - Neuroscience 2019Quote: RNA from brains of 3-month-old zebrafish was extracted in 500μl of Trizol (Invitrogen) and purified by adding 100μl of chloroform ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from adult zebrafish and mouse tissues was isolated using TRIzol reagent (Invitrogen, 15596026) as described previously (Lo et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Larval zebrafish (6 to 7dpf) were paralyzed with alpha-bungarotoxin 2mg/ml (Invitrogen, Waltham, USA), then embedded dorsal side up in 50μl of 1.2% low-melting point agarose (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Zebrafish larvae were anaesthetised with 0.017% MS-222 and embedded in UltraPure LMP Agarose (Invitrogen) on a microscope slide (1.4-1.6 mm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ZMEL1 zebrafish melanoma cells were grown in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) medium supplemented with 1% Glutamax ...
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bioRxiv - Physiology 2020Quote: ... 20 zebrafish larvae digestive tracts were dissected and pooled into 1 mL TRIzol (ThermoFisher, 15596026). mRNA was then isolated with isopropanol precipitation and washed with 70% ethanol ...