Labshake search
Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from zebrafish embryos using TriZol (Invitrogen). cDNA was synthesized using Superscript III kit (Invitrogen) ...
-
bioRxiv - Developmental Biology 2021Quote: ... the zebrafish apo14 promoter46 was cloned into pDONRP4-P1R (Invitrogen), human APOB25 and APOB34 44 were cloned into pDONR221 (Invitrogen) ...
-
bioRxiv - Developmental Biology 2020Quote: RNA was extracted from zebrafish embryos using Trizol reagent (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: Data were collected from 138 Genechip Zebrafish Genome arrays (Affymetrix) across four conditions ...
-
bioRxiv - Biophysics 2023Quote: Zebrafish embryos were injected 1 nL of microspheres (F8816, Thermofisher) at 1:5 of the stock solution at the one-cell zygote stage in order to ensure proper distribution of microspheres ...
-
bioRxiv - Developmental Biology 2024Quote: ... and in zebrafish with klf2a: Dr03138475_g1 (ThermoFisher Scientific, Cat.#: 4331182). The transcript levels were normalized to GAPDH with human Hs99999905_g1 ...
-
bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
-
bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
-
bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
-
bioRxiv - Immunology 2019Quote: ... Recombinant proteins were biotinylated with EZ-Link Sulfo NHS-LC-LC biotin (Thermo Fisher) at a 50 molar excess for 30 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant Tau proteins were biotinylated with 5 molar excess of NHS-biotin conjugates (Thermofisher) during 4 hours at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
-
bioRxiv - Biochemistry 2020Quote: ... recombinant N proteins were coated on 96 well flatbottom immunosorbent plates (Nunc Immuno MaxiSorp) at a concentration of 500 ng/mL ...
-
bioRxiv - Cell Biology 2022Quote: Purified recombinant proteins were labelled using amine reactive NHS ester dyes (Thermo Fisher Scientific). Alexa fluorophores used to label proteins include ...
-
bioRxiv - Immunology 2022Quote: Recombinant proteins were produced using the Freestyle™ 293 Expression System (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... All the recombinant proteins were transformed and expressed in E.coli BL21 (DE3) (Invitrogen, USA).
-
bioRxiv - Microbiology 2023Quote: ... Recombinant BA.5 and BQ.1.1 RBD proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was resolved by NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen; NP0322BOX) to determine identity and purity ...
-
bioRxiv - Biochemistry 2023Quote: The following recombinant proteins were purchased from commercial sources: alkaline phosphatase (Thermo Scientific #EF0651), Antarctic phosphatase (New England BioLabs ...
-
bioRxiv - Immunology 2023Quote: ... and recombinant Cas9 protein via electroporation using the Neon transfection system (Thermo Fisher Scientific). The following day ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific) and amylose resin (NEB) ...
-
bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
-
bioRxiv - Cell Biology 2024Quote: ... after coating with 50 μg/μL Vitronectin (VTN-N) recombinant human protein (Gibco, A14700). PSCs were passed every three days ...
-
bioRxiv - Microbiology 2024Quote: Recombinant ACE2 protein was coated on 96-well NUNC Maxisorp plates (Thermo Fisher Scientific) at 100 ng per well overnight at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Chromatin was cleared with recombinant protein G– conjugated agarose beads (Invitrogen, cat# 15920-010) and subsequently immunoprecipitated with H3K27ac antibody (Active Motif ...
-
bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
-
bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
-
bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
-
bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
-
bioRxiv - Developmental Biology 2019Quote: ... Zebrafish embryos were anesthetized in tricaine (0.013% w/v; Fisher Scientific) diluted in embryo water at 72 hpf ...
-
bioRxiv - Genomics 2020Quote: RNA was extracted from zebrafish embryos with 500μl TRIzol (Invitrogen, ThermoScientific), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Zebrafish were anesthetized in 0.03% tricaine buffer (MS-222; Fisher Scientific) and placed on a moist tissue paper under a dissecting scope (Leica E65S) ...
-
bioRxiv - Immunology 2022Quote: ... Zebrafish larvae were fixed in 4% paraformaldehyde solution (PFA, ThermoFisher Scientific) overnight at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... 30 zebrafish embryos were homogenized in 500 µl of TRIzol (Invitrogen) and total RNA was extracted according to the instructions of the manufacturer ...
-
bioRxiv - Biochemistry 2020Quote: Total mRNA was isolated from Zebrafish embryos by using TRIZOL (Invitrogen). 2 μg of total mRNA was retro-transcribed with Superscript (Invitrogen) ...
-
bioRxiv - Neuroscience 2019Quote: ... Then total RNA was isolated from zebrafish larvae by Trizol (Invitrogen) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA from zebrafish tissues was isolated simultaneously using TRIzol (Invitrogen) and RNA clean & concentrator kit (Zymo Research) ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from zebrafish adult hearts using TRIzol (Invitrogen) and stabilized in RNA later (Thermo Fisher ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the zebrafish embryos were homogenized in TRIzol reagent (Thermo Fisher Scientific). Total RNA from 20 pooled embryos were collected at 6 dpf using TRIzol and RNeasy spin columns (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
-
bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
-
bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
-
bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
-
bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
-
bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
-
bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...