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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Systems Biology 2023Quote: ... ABE8e was expressed as an 8xHis tagged protein from a rhamnose-inducible promoter in BL21-Star DE3 cells with low RNase activity (Thermo Fisher Scientific). At an OD600 of ∼0.8 ...
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression constructs coding for His6/SUMO tagged fusion proteins of rat SHANK3 were generated in pET-SUMO (Thermo Scientific) as described 14 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
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bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg HexaPro) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Bioengineering 2022Quote: ... affinity purified using C-tag affinity resin (Thermo Fisher) using an AKTA chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300, Thermofisher Scientific, Clone 30-F11), anti-LY6G-V450 (1:300 ...
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bioRxiv - Immunology 2023Quote: Cells were stained for viability (Supplementary table 1 and Supplementary table 4) and blocked for CD16/32 with anti-human FC Receptor binding inhibitor (ThermoFisher Scientific, 14-9161-73) in PBS for 15 minutes at room temperature (Spn ...
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bioRxiv - Neuroscience 2019Quote: ... pipettes (1.5 mM KG33) were filled with intracellular solution supplemented with the low-affinity calcium-indicator Fluo-5F (Life Technologies, 100 µM). Fluo-5F has a reported kd for Ca2+ of ∼ 0.70 µM in 1 mM Mg2+ at 30°C (Woodruff et al ...
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bioRxiv - Bioengineering 2020Quote: ... Pro + using an optimized autoinduction media and purified by protein A affinity chromatography similarly to Fabs.30 VH-Fc were expressed in Expi293 BirA cells using transient transfection (Expifectamine, Thermo Fisher Scientific). 4 days after transfection ...
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bioRxiv - Plant Biology 2019Quote: ... pEF1-MCS-3Myc [BamHI-NotI-3Myc-stop fragment was inserted between KpnI and XbaI sites of pEF1/myc-His B vector (Invitrogen)] was generated ...
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bioRxiv - Microbiology 2021Quote: ... Amplified fragments were digested with KpnI and XhoI and cloned into the pre-cut pcDNA4/myc-His A vector (Invitrogen) by using the DNA ligation Kit (Takara) ...
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bioRxiv - Biophysics 2020Quote: ... the sections were incubated with Alexa Fluor 647 goat anti-rabbit lgG (1:1000 dilution) (Invitrogen, CA,USA) for 1 hr in darkness at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... washed and added in secondary antibody (1:500, donkey anti-rabbit lgG, AlexaFlour 555, ThermoFisher Scientific A-31572). Before mounting ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked with CD16/CD32 clone 93 (Thermo Fisher Scientific Cat# 14-0161-86 ...
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bioRxiv - Immunology 2023Quote: ... After block with anti-mouse CD16/CD32 (Invitrogen, ThermoFisher Scientific) and stain with a viability dye (GhostDye Violet 510 ...
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bioRxiv - Immunology 2023Quote: ... After block with anti-mouse CD16/CD32 (Invitrogen, ThermoFisher Scientific) and stain with a viability dye (GhostDye Violet 510 ...
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bioRxiv - Neuroscience 2023Quote: ... and CD16/CD32(1/200, ThermoFisher Scientific, #14-0161-82). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-transferrin receptor (Invitrogen). For the screening of compounds with the potential to rescue GlyT2 defective phenotypes the following chemicals were used ...
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bioRxiv - Cell Biology 2023Quote: ... Transferrin receptor (Invitrogen, 13-6890), NHE5 (PA5-37222) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Biochemistry 2020Quote: ... Cytosolic extracts were generated as described above and the His6-tagged proteins purified by metal affinity chromatography and gravity flow using 0.5 ml HisPur™ cobalt superflow agarose (Thermo Fisher Scientific™, Waltham, USA) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Sf9 cells for generation of baculovirus and expression of recombinant antibody fragment are from Thermo Fisher (12659017, lot 421973). The cells were not authenticated experimentally for these studies ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 12.5% filtered rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryo with magnetic beads injected was placed into the customized imaging chamber and immobilised by DMEM without phenol red containing 12.5% rat serum and 1% low-melt agarose (Invitrogen). The temperature of the imaging chamber was maintained at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Pathology 2019Quote: ... the lung tissue was cut into fragments of approximately 1 mm2 and distributed among 6-well polystyrene plates in low glucose DMEM (Invitrogen)-supplemented culture medium [20% heat inactivated FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Bioengineering 2023Quote: Rhodamine-tagged dextran (Invitrogen) was encapsulated into AlgLung and S-AlgLung hydrogels at a concentration of 0.5 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...