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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... and AcGFP1 DNA fragments were synthesized (Gene Universal) and inserted into pAc5.1/V5-His A vector (Invitrogen). The FUS-Venus plasmid was a kind gift from Dr ...
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bioRxiv - Genetics 2023Quote: ... androgen receptor (Invitrogen), PTK2 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: The DNA coding sequences of tagged recombinant GITRL and OX40L were produced by GeneArt Gene Synthesis service (ThermoFisher Scientific). The sequences of GITRL and OX40L constructs contain ...
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Microbiology 2020Quote: ... hACE2 receptor (hACE2 Recombinant Rabbit Monoclonal Antibody, 1:50, Thermo-Fisher, Cat#SN0754) and DAPI nuclear stain (1:20,000, Invitrogen, Carlsbad, CA). The primary antibodies were detected with goat anti-rabbit-AP developed with Permanent Red warp (mach3 Biocare Medical).
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bioRxiv - Immunology 2021Quote: ... FACS-sorted cells were suspended at 0.5×106 cells/mL in RPMI 1640 medium supplemented with 10% HI-FCS and 1% PenStrep (10378016; Gibco). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biophysics 2019Quote: ... Cells were lysed by sonication and recombinant proteins purified by Ni2+ affinity chromatography in Ni-NTA agarose (Thermo Fisher) using 50 mM TrisHCl/500 mM NaCl/10 mM imidazole [ph 8.0] as binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Cancer Biology 2024Quote: ... or Flag-tagged proteins were generated by inserting PCR-amplified fragments into the pcDNA3.1(+) mammalian expression vector (V79020, Invitrogen). Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The amplified DNA fragments were purified from 1.5% UltraPure™ low melting point agarose gel (Invitrogen™). Sequencing was performed in both directions as described above ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... the serum was collected then affinity purified using His-CSAG1 linked to the amino link plus coupling resins (Thermo scientific, #20501) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
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bioRxiv - Microbiology 2021Quote: ... Purification of His-tagged Cg1978 was performed by applying the supernatant to an equilibrated 2 ml Ni-NTA column (Invitrogen, Kalifornien, USA). After washing with 30 ml TNI20 buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: His-tagged relaxins were expressed as secreted proteins in Expi293F cells containing a stably integrated tetracycline repressor (Expi293F tetR, Thermo Fisher Scientific) grown in Expi293 media (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Expi293F™ cells were transiently transfected with His-tagged gB ectodomain plasmids for five days and later harvested with Nickel-NTA resin (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... captured dsRNA was detected by incubating with sulfo-tagged rat anti-mouse IgM antibody (Thermo Fisher Scientific, Waltham, MA) for 1h before washing and adding MSD GOLD Read Buffer A (Meso Scale ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: ... The resulting TGD057 HA-tagged DNA fragment was then cloned into the pTKO-att parasite expression vector (92) by Gateway Recombination Cloning Technology (Invitrogen). The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were collected from the flask, centrifuged (10 min, 150g, RT) and blocked for Fc receptors by using antibody CD16/32 (Invitrogen, 14-0161-82) (1:100) ...
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bioRxiv - Physiology 2022Quote: Changes in [Ca2+]i elicited by MAPs (thereafter referred as Ca2+ transients) were followed with a low affinity impermeant fluorescent calcium dye (Oregon green 488 BAPTA 5N, thereafter, OGB5N; ThermoFisher Scientific). Ca2+ dependent fluorescence transients were measured using the setup previously described [7] ...
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bioRxiv - Genetics 2023Quote: ... standard fragment analysis conditions) with 0.8 ul PCR product is loaded in 9.4 ul Hi-Di Formamide (Applied Biosystems), with 0.1 ul GeneScan 500 LIZ (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... we initially digested the pNL4-3 plasmid with EcoRI and XhoI and subcloned this fragment into pcDNA3.1/myc-His A (Invitrogen). To generate pcDNA3.1 NL4-3 Env TMTR ...
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bioRxiv - Immunology 2021Quote: ... The RBD-6xHIS protein was purified by affinity chromatography of His Tag residues for metals immobilized on the AktaPure system with HisPur ™ Ni-NTA Chromatography Cartridges (Thermo Fisher) column according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... supernatants were loaded into a 5 mL SepFast Ø11mm (Quimigen) packed with CaptureSelect™ IgG-Fc (ms) affinity resin (Thermo Scientific) connected to an Äkta Start Chromatograph (Cytiva) ...
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bioRxiv - Immunology 2022Quote: ~3 mL of serum from CV-A21-immunized or naïve mice was diluted in 97 mL of PBS and ran over a column pre-packed with CaptureSelect IgG-Fc (Multispecies) Affinity Matrix (ThermoFisher Scientific). IgGs were eluted with 0.1 M glycine buffer (pH 3.0 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The PVDF membrane was washed with TBST buffer and incubated with HPR-Anti rabbit lgG (Thermo Scientific) for 1h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...