Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system (Applied Biosystems). Expression of genes was normalised to three housekeeping genes (Gapdh ...
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bioRxiv - Genomics 2020Quote: ... cDNA was diluted 20-fold in PCR-grade water and subjected to real-time quantitative PCR (qPCR) using SYBR Green PCR Master Mix (ThermoFisher 4309155) with gene specific ...
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bioRxiv - Genomics 2019Quote: We diluted mouse genomic DNA to 5 ng/ul and performed quantitative PCR using an Applied Biosystems 7500 Fast Real-Time PCR instrument and Power SYBR Green PCR Master Mix (Applied Biosystems) with the following cycling conditions ...
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bioRxiv - Physiology 2019Quote: ... Quantitative RT-PCR was carried out with either TaqMan™ Universal PCR Master Mix or SYBR Green PCR master mix on the QuantStudio 7 Flex Real time PCR system (Applied Biosystems). All reactions were carried out in either duplicate or triplicate and Ct values were obtained ...
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bioRxiv - Plant Biology 2020Quote: We performed the quantitative reverse transcription-PCR (qRT-PCR) assays using SybrGreen Takara Master Mix in a 7500 Fast Real-Time PCR system (Applied Biosystems). For PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Transcripts abundance was determined by reverse transcription quantitative PCR (qRT-PCR) using the SYBR® Green RT-PCR assay (Applied Biosystems). Amplification ...
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bioRxiv - Cell Biology 2021Quote: ... was used for quantitative real-time PCR using either StepOne Plus Real-Time PCR system or Quant Studio 12K Flex Real-Time PCR Sytem thermocycler (Applied Biosystems). For each reaction technical duplicates and at least three biological replicates per experiment were performed ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). Primers were based on previous studies (Ding et al. ...
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bioRxiv - Cell Biology 2022Quote: ... real-time PCR was performed using Powerup SYBR Green PCR master mix (A25743) and a Step-One Plus realtime PCR machine (Applied Biosystems). Human Actin expression was used for normalization.
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bioRxiv - Pathology 2022Quote: ... Ten nanograms of cDNA was added to a 20-μL PCR reaction using SYBR Green PCR Master Mix or TaqMan Fast Universal PCR Master Mix (Applied Biosystems) on StepOne Plus System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: Real-time PCR was performed using the Applied Biosystems 7900HT Fast Real-Time PCR System with SYBR green PCR Master Mix (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was cloned into the pCR Blunt II-TOPO vector using the Zero Blunt TOPO PCR cloning kit (Thermo Fisher). The LINE-1 promoter-luciferase reporter plasmid was constructed by subcloning the promoter fragment into the KpnI-XhoI restriction site of the pGL4.11[luc2P] vector (Promega ...
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bioRxiv - Immunology 2020Quote: ... Quantitative PCR was performed with QuantaFast SYBR Green PCR mix (Qiaqen) on a 7500 Fast Real-Time PCR system (Applied Biosystems). The following primers were used: mGAPDH F ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real time-PCR (QRT-PCR) was run on a 7300 real-time PCR system (Applied Biosystems – Life Technologies, Carlsbad, CA). mRNA levels were normalized to 3 housekeeping genes ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real time-PCR (QRT-PCR) was run on a 7300 real-time PCR system (Applied Biosystems – Life Technologies, Carlsbad, CA). mRNA levels were normalized to 3 housekeeping genes ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed in 384 well PCR plates in an ABI 9700 PCR system (Applied Biosystems Inc., Foster City, CA, USA), and consisted of initial denaturation for 10 min at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative-Real-Time-PCR (qRT-PCR) was performed in a 7500 Fast Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific) using TaqMan® Universal PCR Master Mix ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time PCR was performed on the QuantStudio 7 Flex Real-Time PCR System using Power SYBR Green PCR Master Mix (Applied Biosystems). The PCR conditions were 950C for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... Quantitative real time PCR (qRT-PCR) reactions were performed in the StepOnePlus or Quant Studio6 Flex Real Time PCR System (ABI, Thermo Fisher) with ten-fold dilution of cDNA and 200 nM of each primer using the SYBR Select PCR Master Mix (ABI ...
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bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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bioRxiv - Microbiology 2019Quote: ... For cloning of UGV-1 GPC and HISV GPC we used RT-PCR amplification (reverse transcription with RevertAid Reverse Transcriptase, ThermoFisher Scientific; PCR with Phusion Flash High-Fidelity PCR Master Mix, ThermoFisher Scientific) from RNA extracted (by GeneJET RNA Extraction Kit ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR System and Power SYBR Green PCR Master Mix (Applied Biosystems). Relative liver parasite levels were quantified using the ΔΔCt method comparing levels of P ...
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bioRxiv - Microbiology 2021Quote: ... and 9.25 µL sterile water using the standard Power SYBR Green PCR Master Mix RT-PCR Protocol (Protocol Number 436721) on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher). As positive controls for the WSP and WPCP primer sets ...
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bioRxiv - Molecular Biology 2019Quote: ... the 3’ end of rpc37 was first amplified by PCR and cloned into pCR Blunt II-TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies). Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems). Primer sequences are ACTB (endogenous control ...
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bioRxiv - Biochemistry 2021Quote: ... quantitative real-time PCR was performed in 5 μl of SYBR Green PCR master mix using a real-time PCR system (Applied Biosystems). Amplification products were then analyzed by a melting curve ...
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bioRxiv - Cell Biology 2021Quote: ... we performed quantitative Real-time PCR (qRT-PCR) assays by using SYBR™ Green PCR Master Mix (Applied Biosystems, Waltham, MA). These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR System and Power SYBR Green PCR Master Mix (Applied Biosystems). Relative liver parasite levels were quantified using the ΔΔCt method comparing levels of P ...
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bioRxiv - Plant Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) assays were performed in the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative-Real-Time-PCR (qRT-PCR) was performed in a 7500 Fast Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific) using TaqMan® Universal PCR Master Mix ...
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bioRxiv - Microbiology 2022Quote: ... and bla-SHV) was carried out by conventional polymerase chain reaction (PCR) with PCR master mix (DreamTaq Green PCR Master Mix; Thermo Scientific) using specific primers (Table 1).
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bioRxiv - Neuroscience 2023Quote: ... Real-time polymerase chain reaction (RT-PCR) was performed using SYBR Green PCR Master mix (Applied Technologies) on QuantStudio3 Real-Time PCR System (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was performed using the TaqMan Universal PCR Master Mix (Applied Biosytems) and a 7500 Real-Time PCR System (Applied Biosystems). Each condition was performed in duplicate ...
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bioRxiv - Cancer Biology 2023Quote: ... qRT-PCR was performed using SYBR Green PCR with the StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA). The following primer sets were used ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse-transcription-PCR (qRT-PCR) was performed on a QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems A43182) in 384-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 ng of cDNA was used to perform the qRT-PCR reaction using SYBR green PCR master mix on an ABI Quant Studio 6 Flex Real-Time PCR System (Applied Biosystems). The housekeeping genes GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR (qRT-PCR) was performed on the Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the QuantiTect SYBR Green® RT-PCR kit (204243 ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCR (Q-PCR) analysis was performed using SYBR Green Real-time PCR Master Mix on a Real-Time PCR System (Applied Biosystems). The primer sequences utilized for the Q-PCR amplification are provided in the supplemental Table 2.
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bioRxiv - Pathology 2023Quote: ... Quantitative RT-PCR was performed using a standard TaqMan® PCR protocol on a StepOne real time PCR System (Applied Biosystems) with primers specific for murine Dag1 ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (4368708, ThermoFisher Scientific). Primers for HA (5’- AAACTCTTCGCGGTCTTTCCA-3’ sense sequence and 5’-GATAAGGTAGCTTGGGCTGC-3’ antisense sequence) ...
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Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... The quantitative Reverse Transcriptase-PCR (qRT-PCR) was performed on the QuantStudio™ 12K Flex Real-Time PCR System (Applied Biosystems) with PowerUp™ SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was carried out using a PCR system (StepOne; Applied Biosystems) and the Luna One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed on the ABI 7900HT Real-Time PCR system (Applied Biosystems) using SYBR Green Master mix (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... PCR was conducted on the ProFlex 96-Well PCR System (Thermo Fisher Scientific). Thermal cycling conditions consisted of an initial step at 95 ◻ for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... All PCRs were run on a QuantStudio Real-time PCR machine (Applied Biosystems).
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bioRxiv - Evolutionary Biology 2021Quote: ... CD33m was amplified by PCR using SuperScript III One-Step RT-PCR (Invitrogen) and Gene-specific primers 5’-TTATATGCTAGCGCCACCATGCCGCTGCTGCTACTGCTGC-3’ ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative PCR analyses were performed using SYBR Green PCR Master Mix (Applied Biosystems) and primers listed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative real time PCR was performed on a StepOnePlus PCR machine (Applied Biosystems) using the FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) and their purity was verified on 1% agarose gel with SYBR Safe reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed using Power SYBR® Green PCR master mix (ThermoFisher), primers specific for human Ifnlr1 (79 ...