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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Quantitative PCR was performed on the StepOnePlus™ Real-Time PCR System using SYBR green PCR Master-Mix (Thermo-Fisher Scientific 4368708). Primer sequences can be found in supplementary material Table S1 ...
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bioRxiv - Developmental Biology 2023Quote: The StepOnePlus™ Real-Time PCR System was used for quantitative PCR with SYBR green PCR Master-Mix (Thermo-Fisher Scientific 4368708). Primer sequences are available in supplementary material Table S1 ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed on a LifeTechnologies QuantStudio 7 real-time PCR system using SYBR™ Green PCR Master Mix (Applied Biosystems). Gene expression levels were normalized to Actb or Gapdh ...
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bioRxiv - Microbiology 2023Quote: The purified PCR products were cloned into pCR-XL-2-TOPO™ vectors using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). After incubation ...
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bioRxiv - Microbiology 2023Quote: ... the first round PCR products were diluted 10-fold and a total of 6.4 µL of the diluted PCR products were used for real-time PCR on the QuantStudio 3 Real-Time PCR Systems (Thermo Fisher Scientific) using the Perfecta qPCR ToughMix (QuantaBio) ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression of the indicated target genes was determined by real-time PCR using the TaqMan® Fast universal PCR master mix and 7500 Fast Real-Time PCR System from Applied Biosystems. Based on Ct values ...
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bioRxiv - Neuroscience 2024Quote: ... were further evaluated for the quantitative real-time PCR (qRT-PCR) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification of the long TMEM106B 3’ UTR and total TMEM106B levels was determined using the ΔΔCt method and normalized to two endogenous controls ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a QuantStudio3 real-time PCR system using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific). Primers used for qPCR were previously described [19] ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies) with the SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... OCT3/4 and NANOG mRNA expression were confirmed by quantitative real-time PCR (qRT-PCR) with TaqMan™ assay using StepOnePlus™ Real-Time PCR Systems (Thermo Fisher). Primers and probe sequences are provided in Extended Data Table 5.2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed Real Time quantitative PCR (RT-qPCR) on the Bio-Rad CFX connect Real-Time PCR instrument with SYBR™ Green PCR Master Mix (Thermo Fisher Scientific). We computed the fold changes of the target genes by normalizing to the two reference genes 16S rRNA gene and gyrA with against of their geometric mean81 ...
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bioRxiv - Microbiology 2020Quote: ... These primers were earlier used for conventional PCR and thus optimized prior to the q-PCR SYBR Green assay using RT-PCR system 7500 (Applied Biosystems, CA, USA). The reaction mixture was prepared using the Power SYBR Green PCR Master mix 2X kits (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were analysed with quantitative real-time–PCRs (qRT–PCR) analyses conducted on the StepOnePlus™ Real- Time PCR System (Thermo Scientific, UK) with the Fast SYBR-Green master mix (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Gene expression was analyzed by quantitative reverse transcription PCR (qRT-PCR) analysis on a QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and normalized to endogenous control gene mGUS ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Immunology 2023Quote: ... Gene expression levels were measured by reverse transcription quantitative PCR (RT-PCR) using PowerUp SYBR Green PCR Master Mix (Thermo Fisher Scientific, A25742). Primers are listed in the Supplementary Table S3 and ΔCt was calculated relative to Actb gene while relative gene expression levels were quantified using ΔΔCt method normalizing to unstimulated groups ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product sequences were confirmed by cloning the PCR products into the pCR™4Blunt-TOPO® vector by TOPO cloning (Thermo Fisher) and sequenced using the M13 reverse primer site by sanger sequencing (Eurofins).
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bioRxiv - Molecular Biology 2023Quote: ... The quantitative real-time PCR (qRT-PCR) experiments were performed utilizing the POWRUP SYBR MASTER MIX via a StepOnePlus Real-time PCR instrument (Applied Biosystems, Texas, USA).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Quantitative real-time PCR was performed on a StepOnePlus Real-Time PCR system with TaqMan Fast Universal PCR Master Mix (Thermo Fisher Scientific K.K.) and the following gene-specific primers ...
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bioRxiv - Microbiology 2024Quote: Quantitative real time PCR (qRT-PCR) was performed using an ABI 7500 Fast Dx Real-Time PCR instrument (Applied Biosystems, Waltham, MA, USA). PCR reactions were performed in a 5 uL RNA reaction volume using SYBR™GreenER™ qPCR SuperMix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, United States) with gene-specific primers (Supplementary Table 6) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with Power SYBR Green PCR Master Mix (Applied Biosystems, Japan) using the Thermal Cycler Dice RealTime TP870 (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCRs (qPCR) were performed with SybrGreen dye in StepOnePlus PCR detector (Applied Biosystems). PCR amplification was performed for 40 cycles at 95 °C for 3 s and 60 °C for 30 s with a preceding initial enzyme activation of 20 s at 95 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR analysis was performed using the 7500 Real-Time PCR system (Applied Biosystems) with Taqman 2x Fast universal PCR Master Mix (#4304437 ...
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bioRxiv - Zoology 2021Quote: ... Amplicon PCR reaction was performed using the GeneAmp PCR System 2700 (Thermo Fisher Scientific) and the following cycling conditions ...
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bioRxiv - Zoology 2021Quote: ... Amplicon PCR reaction was performed using the GeneAmp PCR System 9700 (Thermo Fisher Scientific) and the following cycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was done using the ABI 7500 FAST qRT-PCR machine (Applied Biosystems) using the Taqman gene expression (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed with the 7500 Fast Real-Time PCR system (Applied Biosystems™) in duplicate of 20 μl reactions ...
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bioRxiv - Developmental Biology 2021Quote: ... then sequencing the PCR product after cloning via CloneJET PCR cloning kit (Thermo Scientific).
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were done using Phire Plant Direct PCR kit® (Thermo Scientific®) and gDNA from wings according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned using the ExoSAP-IT PCR Product Cleanup kit (Applied Biosystems) following the manufacturer’s protocol and then visualized using agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems; AB4367659). All measurements were performed in triplicate and were standardized to the levels of either HPRT or UBC ...
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR was performed using the SYBR GREEN PCR Master Mix from Applied Biosystems. The target gene mRNA expression was normalized to the expression of two housekeeping genes (HPRT1 and RPL13) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR products were purified with the Gene JET PCR purification kit (Thermo Fisher). Two hundred nanograms of PCR product were denatured and re-annealed in 10x NEB2 buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The final PCR products were purified by PureLink PCR purification kit (Thermo Fisher Scientific) and submitted for next-generation sequencing using Illumina MiSeq PE250.
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bioRxiv - Immunology 2021Quote: ... and qRT-PCR was performed on a StepOnePlus Real-time PCR system (Applied Biosystems) with TB Green Premix Ex Taq II (Takara Cat no.RR820A) ...
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bioRxiv - Genetics 2022Quote: ... We ran RT-PCR in a QuantStudio 3 Real-Time PCR machine (Thermo Fisher) using continuous capture with a 0.025°C/s ramp ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed using SYBR™ Green PCR Master Mix (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was completed with Phusion High-Fidelity PCR Master Mix kit (Thermo Fisher, F531). At the same time ...
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bioRxiv - Molecular Biology 2020Quote: ... all PCR reactions were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific). All mutants were verified by sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were carried out with a 7500 Real-Time PCR System (Applied Biosystems) under standard conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed by using 7900HT fast real time PCR system (Applied Biosystems). Expression of TFRC gene was normalized with actin.
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bioRxiv - Immunology 2022Quote: ... Real-time PCR was performed using SYBRgreen PCR Master Mix (Applied Biosystems, ThermoFisher Scientific) and the ViiA 7 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR was performed using SYBRgreen PCR Master Mix (Applied Biosystems, ThermoFisher Scientific) and the ViiA 7 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR was performed using Power SYBR Green PCR Master Mix (Life Technologies, USA) on ABI ViiA-7TM real-time PCR system with the standard relative quantification mode (2ΔΔCT ...
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bioRxiv - Genomics 2020Quote: qRT-PCRs were performed using Ambion AgPath-ID One Step RT-PCR (Thermo Fisher) according to Centers for Disease Control and Prevention-developed singleplex real-time reverse transcription PCR (rRT-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR amplification was performed on a GeneAmp® 9700 PCR System (Applied Biosystems). Cycling conditions consisted of an initial denaturation at 94°C for 3 min ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were cloned with a TOPO PCR cloning kit (Invitrogen Life Technologies) and sequenced (Eurofins) ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were cloned with a TOPO PCR cloning kit (Invitrogen Life Technologies) and sequenced (Eurofins) ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were cloned with a TOPO PCR cloning kit (Invitrogen Life Technologies) and sequenced (Eurofins) ...